Team:Fatih Turkey/Data Page

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<a href="http://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a>
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<a href="http://2011.igem.org/Team:Fatih_Turkey/Project">Project</a>
<ul>
<ul>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Results">Results</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li>
</ul>
</ul>
</li>
</li>
<li>
<li>
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<a href="http://2011.igem.org/Team:Fatih_Turkey/Biobricks">Biobricks</a>
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<a href="http://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a>
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<ul>
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</li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Team">Team</a>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Devices">Devices</a></li>
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<ul>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a></li>
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                                                                                <li><a  
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</ul>
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href="http://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li>
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</li>
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                                                                                  <li><a  
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>
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href="http://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li>
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                                                                                  <li><a  
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href="http://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li>
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</ul>
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</li>
<li>
<li>
<a href="http://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a>
<a href="http://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a>
<ul>
<ul>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li>
<li><a href="http://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li>
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                                                                                <li><a
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href="http://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>
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<li><a href="http://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li>
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                                                            </ul>
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<div>
<div class="meta-left-full">
<div class="meta-left-full">
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<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">We designed E.coli device to express reflectin protein which gives different colors in different wavelength, color change according to thickness of colony.</p>
 
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<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">B.Subtilus contain LALF device , produces Anti-LPS vector  which binds E.coli  membrane Lipopolysaccharide and stop growth of E.coli</p>
 
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<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">Because of LALF E.colican not growth and no reflectin produced , thus no color change occur due to
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<center><img src="http://2011.igem.org/wiki/images/1/1b/Data700.png"></center>
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LALF.</p>
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<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">Data for our favorite new parts:</p>
 
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<ol>
 
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<li><b>Limulus anti-LPS factor(LALF) – BBa_K541505:</b> Gene of this protein is sequenced and confirmed. Moreover, we added a GFP sequence after LALF and inserted in E.coli. We saw green colonies which show us that LALF is produced.</li>
 
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<li><b>LALF protein with Tat signal peptide compatible with E.coli – BBa_K541545:</b> This part includes an IPTG inducible E.coli promoter. The colonies that had been incubated in the plate with IPTG were smaller than the ones in the plate without IPTG. In addition, we have sequenced this part and confirmed it. Also, we produced its cDNA and checked that the part is produced.</li>
 
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<li><b>LALF protein with SacB signal peptide compatible with B.subtilis – BBa_K541915:</b> Culture of B.subtilis with this part and its supernatant were added on different E.coli with J04450 promoter plates. No E.coli colony grew in the plate.Also, cDNA of this part is produced and corrected. Thus, our protein is synthesized.</li>
 
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<li><b>Reflectin1A with E.coli compatible promoter – BBa_K541596: </b>After gel electrophoresis, we saw correct bands and confirmed this part. Also, after the transformation to TOP10 E.coli strain, we saw white colonies in the plate which shows us thatthe part is confirmed.</li>
 
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</ol>
 
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<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">Data for pre-existing parts:</p>
 
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<p><strong>DATA FOR OUR FAVORITE NEW PARTS</strong></p>
<ol>
<ol>
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<li><b>Shuttle vector BBa_K090403:</b> We have upgraded this part by inserting BBa_J04450 into BBa_K090401 to use it for the identification in our experiments. (BBa_K541800) We saw red colonies during our experiments.</li>
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  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541515">Main Page Limulus anti-LPS factor (LALF) for Bacillus Subtilis,  BBa_K541515:</a></strong> Part  contains constitutive promoter veg, RBS spoVG and SacB signal peptide sequence.  LALF sequence attached to all of these subparts. Signal peptide is inserted in order to secrete our protein outside of cell. This part has been characterized  via gel electrophoresis, cDNA and DNA sequencing. It is the main part that we  used in our assays.</li>
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<li><b>B.subtilis compatible promoter BBa_K143053: </b>We added GFP sequence after the promoter and saw green colonies during our experiments.</li>
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  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541545">Main Page Limulus  Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible), BBa_K541545:</a> </strong>PlacI IPTG inducible promoter, RBS  (BBa_B0034) and Tat signal peptide sequence in order to synthesize the protein  outside are the main components of this part. LALF is attached to all these  subparts. This part is designed for testing if E.coli inhibits its own growth  via LALF. <strong></strong></li>
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<li><b>E.coli compatible promoter – BBa_J04500: </b>We added GFP sequence after the promoter and saw green colonies during our experiments.</li>
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  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541915">Main Page - Multi-host vector pTG262 converted to BioBrick vector with  LALF protein and SacB signal peptide; BBa_K541915:</a> </strong>Gene sequence of this part is same with  BBa_K541515. Only difference is the plasmid backbone we use in the design of  this part. (BBa_I742123) This backbone is compatible with both E.coli and B.subtilis. This allows that the part can be used in both kinds.</li>
</ol>
</ol>
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<p><strong>DATA FOR PARTS THAT WE  UPGRADED</strong></p>
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<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">We have also characterized the following parts:</p>
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<ol>
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  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541800">Experience - B.subtilis and E.coli  Episomal Shuttle Vector with Constitutive RFP to express in E.coli;  BBa_K541800: </a></strong>Original  forms of BBa_K090401 and BBa_K090402 are upgraded by inserting RFP sequence  that can be used for clear characterization and experiments. <strong></strong></li>
 +
</ol>
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<p><strong>DATA FOR PARTS THAT WE  HAVE CHARACTERIZED</strong></p>
<ol>
<ol>
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<li><b>Reflectin 1A – BBa_K541506:</b> Sequencing is performed for this protein and the part is confirmed.</li>
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  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541501">Promoter Pveg, RBS spoVG and SacB  signal peptide for B. Subtilis; (BBa_K541501):</a> </strong>This is the subpart of SacB signal peptide  which is commonly used in our BioBricks. Signal peptide is used for the  secretion of our protein outside of the cell. Promoter and RBS are compatible  with B.subtilis.<strong></strong></li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541502">Promoter Pveg, RBS spoVG and LipA  signal peptide for B. Subtilis; (BBa_K541502):</a> </strong>Subpart of LipA signal peptide is designed  alternatively for BBa_K541501. Promoter and RBS are compatible with B.subtilis.<strong></strong></li>
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  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541503">Constitutive Strong promoter, Strong  RBS and TAT signal peptide; (BBa_K541503):</a></strong> The aim of design of this part is same with  BBa_K541501 and BBa_K541502. This part is compatible with only E.coli because  of its promoter and RBS. Tat signal peptide is inserted in this gene.</li>
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  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541504">IPTG inducible PlacI, RBS and TAT  signal peptide; (BBa_K541504):</a> </strong>It is same with BBa_K541503. Differently, we used IPTG inducible PlacI  promoter with E.coi compatible RBS.<strong></strong></li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541505">Limulus anti-LPS factor (LALF);  (BBa_K541505):</a></strong> LALF  subpart that we inserted in our BioBricks which are related to LALF. It has  been synthesized in vitro in order to prevent horseshoe crab death, sea  creature that hosts LALF. For more information; visit our <a href="http://2011.igem.org/Team:Fatih_Turkey/LALF">LALF page.</a></li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541506">Reflectin1A  from Cephalopod</a></strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541506"><strong>; (BBa_K541506):</strong></a><strong> </strong>Reflectin is aimed to use as a biosensor in our  project. This is the subpart of parts for reflectin. <strong></strong></li>
</ol>
</ol>
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 +
</div>
</div>

Latest revision as of 19:29, 28 October 2011

deneme baslik

DATA FOR OUR FAVORITE NEW PARTS

  1. Main Page – Limulus anti-LPS factor (LALF) for Bacillus Subtilis, BBa_K541515: Part contains constitutive promoter veg, RBS spoVG and SacB signal peptide sequence. LALF sequence attached to all of these subparts. Signal peptide is inserted in order to secrete our protein outside of cell. This part has been characterized via gel electrophoresis, cDNA and DNA sequencing. It is the main part that we used in our assays.
  2. Main Page – Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible), BBa_K541545: PlacI IPTG inducible promoter, RBS (BBa_B0034) and Tat signal peptide sequence in order to synthesize the protein outside are the main components of this part. LALF is attached to all these subparts. This part is designed for testing if E.coli inhibits its own growth via LALF.
  3. Main Page - Multi-host vector pTG262 converted to BioBrick vector with LALF protein and SacB signal peptide; BBa_K541915: Gene sequence of this part is same with BBa_K541515. Only difference is the plasmid backbone we use in the design of this part. (BBa_I742123) This backbone is compatible with both E.coli and B.subtilis. This allows that the part can be used in both kinds.

DATA FOR PARTS THAT WE UPGRADED

  1. Experience - B.subtilis and E.coli Episomal Shuttle Vector with Constitutive RFP to express in E.coli; BBa_K541800: Original forms of BBa_K090401 and BBa_K090402 are upgraded by inserting RFP sequence that can be used for clear characterization and experiments.

DATA FOR PARTS THAT WE HAVE CHARACTERIZED

  1. Promoter Pveg, RBS spoVG and SacB signal peptide for B. Subtilis; (BBa_K541501): This is the subpart of SacB signal peptide which is commonly used in our BioBricks. Signal peptide is used for the secretion of our protein outside of the cell. Promoter and RBS are compatible with B.subtilis.
  2. Promoter Pveg, RBS spoVG and LipA signal peptide for B. Subtilis; (BBa_K541502): Subpart of LipA signal peptide is designed alternatively for BBa_K541501. Promoter and RBS are compatible with B.subtilis.
  3. Constitutive Strong promoter, Strong RBS and TAT signal peptide; (BBa_K541503): The aim of design of this part is same with BBa_K541501 and BBa_K541502. This part is compatible with only E.coli because of its promoter and RBS. Tat signal peptide is inserted in this gene.
  4. IPTG inducible PlacI, RBS and TAT signal peptide; (BBa_K541504): It is same with BBa_K541503. Differently, we used IPTG inducible PlacI promoter with E.coi compatible RBS.
  5. Limulus anti-LPS factor (LALF); (BBa_K541505): LALF subpart that we inserted in our BioBricks which are related to LALF. It has been synthesized in vitro in order to prevent horseshoe crab death, sea creature that hosts LALF. For more information; visit our LALF page.
  6. Reflectin1A from Cephalopod; (BBa_K541506): Reflectin is aimed to use as a biosensor in our project. This is the subpart of parts for reflectin.