Team:Fatih Turkey/Data Page

From 2011.igem.org

(Difference between revisions)
(Created page with "Data Page")
 
(12 intermediate revisions not shown)
Line 1: Line 1:
-
Data Page
+
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
 +
<title>deneme baslik</title>
 +
<style>
 +
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{
 +
  display:none;
 +
}
 +
#menubar{
 +
background-color: transparent;
 +
font-size: 85%;
 +
top: 10px;
 +
position: absolute;
 +
margin-top: -12px;
 +
}
 +
 
 +
div#content{
 +
padding: 0 !important;
 +
background: transparent;
 +
border: none !important;
 +
width: auto;
 +
}
 +
 
 +
#menubar li a {
 +
color: #FFFFFF;
 +
background-color: transparent;
 +
}
 +
#globalWrapper{
 +
margin-top:5px;
 +
}
 +
#top-section{
 +
height: 20px !important;
 +
border: none !important;
 +
}
 +
body {
 +
font-size: 12px;
 +
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;
 +
color: #444;
 +
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');
 +
}
 +
body,p,pre,div,span{
 +
line-height: 160%;
 +
color: #333;
 +
}
 +
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,
 +
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td
 +
{
 +
margin:0;
 +
padding:0;
 +
border:0;
 +
font-weight:inherit;
 +
font-style:inherit;
 +
font-family:inherit;
 +
vertical-align:baseline;
 +
}
 +
 
 +
em{
 +
font-style:italic;
 +
}
 +
table{
 +
margin-top: 15px;
 +
margin-bottom: 15px;
 +
}
 +
hr{
 +
border: 0;
 +
color: #eee;
 +
background-color: #eee;
 +
height: 1px;
 +
margin-bottom: 20px;
 +
}
 +
img.centered {
 +
display: block;
 +
margin-left: auto;
 +
margin-right: auto;
 +
}
 +
img.alignnone {
 +
margin: 0px 0px 5px 5px;
 +
display: inline;
 +
}
 +
img.alignright {
 +
margin: 0px 0px 5px 10px;
 +
display: inline;
 +
}
 +
 
 +
img.alignleft {
 +
margin: 0px 10px 5px 0px;
 +
display: inline;
 +
}
 +
.alignright {
 +
float: right;
 +
}
 +
 
 +
.alignleft {
 +
float: left;
 +
}
 +
.aligncenter{
 +
margin-left: auto;
 +
margin-right: auto;
 +
}
 +
h1,h2,h3,h4,h5,h6{
 +
  margin-top: 15px;
 +
  margin-bottom: 15px ;
 +
  color: #333;
 +
  line-height: 1.5em;
 +
}
 +
h1 {
 +
font-size: 30px;
 +
}
 +
h2 {
 +
font-size: 25px;
 +
}
 +
h3 {
 +
font-size: 20px;
 +
}
 +
h4 {
 +
font-size: 18px;
 +
}
 +
h5 {
 +
font-size: 16px;
 +
}
 +
 
 +
h6 {
 +
font-size: 15px;
 +
}
 +
a{
 +
text-decoration: none;
 +
}
 +
a{
 +
color: #2f86c4;
 +
}
 +
a:hover{
 +
color: #4172ab;
 +
}
 +
#header .logo{
 +
margin-top: 41px;
 +
margin-left: 40px;
 +
}
 +
#footer{
 +
background: #707070;
 +
border-left: 1px #888 solid;
 +
border-right: 1px #888 solid;
 +
width: 988px;
 +
margin: 0px auto 0px;
 +
overflow: hidden;
 +
}
 +
#footer div{
 +
color: #bbb;
 +
}
 +
#footer a{
 +
color: #c4c5cc;
 +
text-decoration: none;
 +
}
 +
#footer a:hover{
 +
color: #666;
 +
}
 +
 
 +
.footer-sh-wrapper {
 +
    height: 22px;
 +
    margin: auto;
 +
    position: relative;
 +
    width: 990px;
 +
}
 +
.footer-sh-left {
 +
    background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;
 +
    height: 112px;
 +
    left: 0;
 +
    position: absolute;
 +
    top: -90px;
 +
    width: 10px;
 +
}
 +
.footer-sh {
 +
    background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;
 +
    bottom: 0;
 +
    height: 22px;
 +
    left: 10px;
 +
    position: absolute;
 +
    width: 970px;
 +
}
 +
.footer-sh-right {
 +
    background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;
 +
    height: 112px;
 +
    position: absolute;
 +
    right: 0;
 +
    top: -90px;
 +
    width: 10px;
 +
}
 +
 
 +
.copyright-open{
 +
width: 988px;
 +
height: 0px;
 +
margin:auto;
 +
display: block-inline;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
color: #333333;
 +
}
 +
#header-whole{
 +
 
 +
position: relative;
 +
z-index: 200;
 +
width: 980px;
 +
margin: 0 auto;
 +
}
 +
#header{
 +
height: 100px;
 +
clear: both;
 +
background: #f9f9f9;
 +
margin: 0 auto;
 +
    width: 970px;
 +
}
 +
#header .logo{
 +
float: left;
 +
margin-top: 15px;
 +
margin-left: 20px;
 +
}
 +
 
 +
#container{
 +
width: 970px;
 +
margin: 0px auto 0px;
 +
background: none repeat scroll 0 0 #F9F9F9;
 +
}
 +
.copyright-open{
 +
border-top: 1px #a8a8a8 solid;
 +
border-bottom: 1px #888 solid;
 +
border-left: 1px #888 solid;
 +
border-right: 1px #888 solid;
 +
}
 +
.nav-wrapper{
 +
margin: 0px 90px 0px 0px;
 +
float:right;
 +
}
 +
.ddsmoothmenu ul{
 +
padding: 0px;
 +
list-style-type: none;
 +
background: #666666;
 +
}
 +
.ddsmoothmenu ul li{
 +
position: relative;
 +
display: block;
 +
float: left;
 +
}
 +
.ddsmoothmenu ul li a{
 +
line-height: 140%;
 +
overflow: hidden;
 +
text-align: left;
 +
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;
 +
word-spacing: 1px;
 +
font-size: 14px;
 +
font-weight: normal;
 +
display: block; /*background of menu items (default state)*/
 +
padding: 34px 20px 10px 17px;
 +
text-decoration: none;
 +
}
 +
.ddsmoothmenu ul li a span {
 +
font-weight: normal;
 +
color: #888;
 +
font-size: 10px;
 +
clear: both;
 +
display:block;
 +
width: 60px;
 +
}
 +
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/
 +
display: inline-block;
 +
}
 +
 
 +
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{
 +
color: #333;
 +
}
 +
 
 +
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/
 +
color: #888;
 +
}
 +
 
 +
.ddsmoothmenu ul li a:hover{
 +
color: #888;
 +
}
 +
 +
/*1st sub level menu*/
 +
.ddsmoothmenu ul li ul{
 +
position: absolute;
 +
left: 0;
 +
visibility: hidden;
 +
display: none; /*collapse all sub menus to begin with*/
 +
border: 1px #222 solid;
 +
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;
 +
}
 +
 
 +
/*Sub level menu list items (undo style from Top level List Items)*/
 +
.ddsmoothmenu ul li ul li{
 +
text-align:left;
 +
float: none;
 +
background: url('img/bg-menu2.png');
 +
}
 +
 
 +
.ddsmoothmenu ul li ul li a{
 +
color: #fff;
 +
font-size: 12px;
 +
}
 +
.ddsmoothmenu ul li ul li  a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/
 +
color: #fff;
 +
}
 +
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{
 +
color: #fff;
 +
}
 +
.ddsmoothmenu ul li ul li a:hover{
 +
color: #ffc;
 +
}
 +
.ddsmoothmenu ul li ul li ul{
 +
top: 0;
 +
}
 +
 
 +
/* Sub level menu links style */
 +
.ddsmoothmenu ul li ul li a{
 +
width: 160px; /*width of sub menus*/
 +
padding: 0px 0px 0px 10px;
 +
height: 38px;
 +
line-height: 320%;
 +
border-left: 1px #555 solid;
 +
overflow: hidden;
 +
}
 +
 
 +
/* Holly Hack for IE \*/
 +
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/
 +
 
 +
.copyright{
 +
width: 930px;
 +
color: #959595;
 +
background: #eee;
 +
clear: both;
 +
line-height: 190%;
 +
text-align: right;
 +
padding: 10px 20px 10px;
 +
margin: 0px auto 0px;
 +
}
 +
.copyright a{
 +
color: #bbb;
 +
text-decoration: none;
 +
}
 +
.copyright a:hover{
 +
color: #ccc;
 +
}
 +
.bar-title-whole{
 +
overflow: visible;
 +
position: relative;
 +
width: 990px;
 +
margin: auto;
 +
z-index: 10;
 +
}
 +
.bar-title{
 +
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;
 +
border-left: 1px #8a8a8a solid;
 +
border-right: 1px #8a8a8a solid;
 +
 
 +
width: 918px;
 +
height: 122px;
 +
margin: auto;
 +
overflow: hidden;
 +
padding-left: 35px;
 +
padding-right: 35px;
 +
color: #ddd;
 +
line-height: 150%;
 +
font-size: 12px;
 +
 +
position: relative;
 +
z-index: 10;
 +
}
 +
 
 +
.bar-title h2{
 +
color: #fff;
 +
margin-top: 15px;
 +
margin-bottom: 30px !important;
 +
}
 +
.bar-title h5{
 +
color: #eee;
 +
}
 +
.bar-title-sh-left{
 +
width: 10px;
 +
height: 112px;
 +
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
left: 0px;
 +
}
 +
.bar-title-sh{
 +
width: 970px;
 +
height: 8px;
 +
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
left: 10px;
 +
}
 +
.bar-title-sh-right{
 +
width: 10px;
 +
height: 112px;
 +
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;
 +
display: block-inline;
 +
position: absolute;
 +
top: 122px;
 +
right: 0px;
 +
}
 +
#page{
 +
    width: 970px;
 +
    margin: 0px auto 0px;
 +
    overflow: hidden;
 +
    background: #f9f9f9;
 +
   
 +
}
 +
 
 +
#page .page-wrapper{
 +
margin: 15px 30px 0px 30px;
 +
width: 910px;
 +
overflow: hidden;
 +
float: left;
 +
}
 +
#page .page-wrapper-right{
 +
margin: 30px 40px 0px 25px;
 +
width: 935px;
 +
overflow: hidden;
 +
float: right;
 +
}
 +
#page .title-wrapper h1{
 +
margin-top: 0.5em;
 +
margin-bottom: 0.3em;
 +
}
 +
#page .meta-left-full{
 +
margin: 15px 0px 5px 0px;
 +
overflow: hidden;
 +
}
 +
#page .meta-left-button{
 +
margin: 5px 0px 60px 0px;
 +
overflow: hidden;
 +
}
 +
#page .w600{
 +
width: 600px;
 +
}
 +
 +
</style>
 +
 
 +
 
 +
</head>
 +
<body>
 +
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script>
 +
 
 +
<script>
 +
$(document).ready(
 +
ddsmoothmenu.init({
 +
mainmenuid: "topmenu",
 +
orientation: 'h',
 +
classname: 'ddsmoothmenu',
 +
contentsource: "markup"
 +
}));
 +
 
 +
jQuery(window).load(function() {
 +
setTimeout(function(){
 +
jQuery('#slider').nivoSlider({
 +
pauseTime:4000,
 +
animSpeed:600,
 +
captionOpacity: 0.8,
 +
pauseOnHover:true,
 +
directionNav:true,
 +
directionNavHide:true,
 +
controlNavThumbs:true,
 +
controlNavThumbsFromRel:true,
 +
});
 +
}, 1000),
 +
setTimeout(function(){
 +
/* For slider shortcode */
 +
jQuery('#slider2').nivoSlider({
 +
pauseTime:4000,
 +
animSpeed:600,
 +
captionOpacity: 0.8,
 +
pauseOnHover:true,
 +
directionNavHide:true });
 +
}, 1000);
 +
 +
});
 +
</script>
 +
 
 +
<div class="body-2">
 +
<!-- Header -->
 +
<div id="header-whole">
 +
<div id="header">
 +
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a>
 +
<!-- Logo -->
 +
<div>
 +
<div class="logo">
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey">
 +
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png">
 +
</a>
 +
</div>
 +
</div>
 +
<!-- Menu -->
 +
<div class="nav-wrapper">
 +
<div class="nav-main">
 +
<div id="topmenu" class="ddsmoothmenu">
 +
<ul id="menu-main" class="menu">
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a>
 +
</li>
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a>
 +
</li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a>
 +
<ul>
 +
                                                                                <li><a
 +
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li>
 +
                                                                                  <li><a
 +
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li>
 +
                                                                                  <li><a
 +
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li>
 +
                                                                                <li><a
 +
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li>
 +
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li>
 +
</ul>
 +
</li>
 +
                                                            </ul>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- End Header -->
 +
<div class="bar-title-whole">
 +
  <div class="bar-title">
 +
      <h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Biobricks</h2>
 +
      <h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Data Page</h5>
 +
    </div>
 +
  <div class="bar-title-sh-left"></div>
 +
  <div class="bar-title-sh"></div>
 +
  <div class="bar-title-sh-right"></div>
 +
        </div>
 +
</div>
 +
<div id="container">
 +
<div id="page">
 +
<div class="page-wrapper">
 +
<!-- post container -->
 +
<div>
 +
<div class="meta-left-full">
 +
 
 +
 
 +
<center><img src="https://static.igem.org/mediawiki/2011/1/1b/Data700.png"></center>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<p><strong>DATA FOR OUR FAVORITE NEW PARTS</strong></p>
 +
<ol>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541515">Main Page – Limulus anti-LPS factor (LALF) for Bacillus Subtilis,  BBa_K541515:</a></strong> Part  contains constitutive promoter veg, RBS spoVG and SacB signal peptide sequence.  LALF sequence attached to all of these subparts. Signal peptide is inserted in  order to secrete our protein outside of cell. This part has been characterized  via gel electrophoresis, cDNA and DNA sequencing. It is the main part that we  used in our assays.</li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541545">Main Page – Limulus  Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible), BBa_K541545:</a> </strong>PlacI IPTG inducible promoter, RBS  (BBa_B0034) and Tat signal peptide sequence in order to synthesize the protein  outside are the main components of this part. LALF is attached to all these  subparts. This part is designed for testing if E.coli inhibits its own growth  via LALF. <strong></strong></li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541915">Main Page - Multi-host vector pTG262 converted to BioBrick vector with  LALF protein and SacB signal peptide; BBa_K541915:</a> </strong>Gene sequence of this part is same with  BBa_K541515. Only difference is the plasmid backbone we use in the design of  this part. (BBa_I742123) This backbone is compatible with both E.coli and  B.subtilis. This allows that the part can be used in both kinds.</li>
 +
</ol>
 +
<p><strong>DATA FOR PARTS THAT WE  UPGRADED</strong></p>
 +
<ol>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541800">Experience - B.subtilis and E.coli  Episomal Shuttle Vector with Constitutive RFP to express in E.coli;  BBa_K541800: </a></strong>Original  forms of BBa_K090401 and BBa_K090402 are upgraded by inserting RFP sequence  that can be used for clear characterization and experiments. <strong></strong></li>
 +
</ol>
 +
<p><strong>DATA FOR PARTS THAT WE  HAVE CHARACTERIZED</strong></p>
 +
 
 +
<ol>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541501">Promoter Pveg, RBS spoVG and SacB  signal peptide for B. Subtilis; (BBa_K541501):</a> </strong>This is the subpart of SacB signal peptide  which is commonly used in our BioBricks. Signal peptide is used for the  secretion of our protein outside of the cell. Promoter and RBS are compatible  with B.subtilis.<strong></strong></li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541502">Promoter Pveg, RBS spoVG and LipA  signal peptide for B. Subtilis; (BBa_K541502):</a> </strong>Subpart of LipA signal peptide is designed  alternatively for BBa_K541501. Promoter and RBS are compatible with B.subtilis.<strong></strong></li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541503">Constitutive Strong promoter, Strong  RBS and TAT signal peptide; (BBa_K541503):</a></strong> The aim of design of this part is same with  BBa_K541501 and BBa_K541502. This part is compatible with only E.coli because  of its promoter and RBS. Tat signal peptide is inserted in this gene.</li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541504">IPTG inducible PlacI, RBS and TAT  signal peptide; (BBa_K541504):</a> </strong>It is same with BBa_K541503. Differently, we used IPTG inducible PlacI  promoter with E.coi compatible RBS.<strong></strong></li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541505">Limulus anti-LPS factor (LALF);  (BBa_K541505):</a></strong> LALF  subpart that we inserted in our BioBricks which are related to LALF. It has  been synthesized in vitro in order to prevent horseshoe crab death, sea  creature that hosts LALF. For more information; visit our <a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF page.</a></li>
 +
  <li><strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541506">Reflectin1A  from Cephalopod</a></strong><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K541506"><strong>; (BBa_K541506):</strong></a><strong> </strong>Reflectin is aimed to use as a biosensor in our  project. This is the subpart of parts for reflectin. <strong></strong></li>
 +
</ol>
 +
 
 +
 
 +
 
 +
</div>
 +
    </div>
 +
      </div>
 +
</div>
 +
</div>
 +
 
 +
<div class="footer-sh-wrapper">
 +
<div class="footer-sh-left"></div>
 +
<div class="footer-sh"></div>
 +
<div class="footer-sh-right"></div>
 +
</div>
 +
<div id="footer">
 +
<style>
 +
#footer img:hover{
 +
opacity:0.6;
 +
}
 +
</style>
 +
<div>
 +
<h3 style="color:#BBB;">Sponsors</h3>
 +
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/>
 +
<a href="http://www.sentegen.com">
 +
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a>
 +
</div>
 +
</div>
 +
<div class="copyright-open">
 +
</div>
 +
<div class="copyright">
 +
2011 © Fatih Medical School
 +
</div>
 +
</div>
 +
 
 +
 
 +
</body>
 +
</html>

Latest revision as of 19:29, 28 October 2011

deneme baslik

Biobricks

Data Page

DATA FOR OUR FAVORITE NEW PARTS

  1. Main Page – Limulus anti-LPS factor (LALF) for Bacillus Subtilis, BBa_K541515: Part contains constitutive promoter veg, RBS spoVG and SacB signal peptide sequence. LALF sequence attached to all of these subparts. Signal peptide is inserted in order to secrete our protein outside of cell. This part has been characterized via gel electrophoresis, cDNA and DNA sequencing. It is the main part that we used in our assays.
  2. Main Page – Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible), BBa_K541545: PlacI IPTG inducible promoter, RBS (BBa_B0034) and Tat signal peptide sequence in order to synthesize the protein outside are the main components of this part. LALF is attached to all these subparts. This part is designed for testing if E.coli inhibits its own growth via LALF.
  3. Main Page - Multi-host vector pTG262 converted to BioBrick vector with LALF protein and SacB signal peptide; BBa_K541915: Gene sequence of this part is same with BBa_K541515. Only difference is the plasmid backbone we use in the design of this part. (BBa_I742123) This backbone is compatible with both E.coli and B.subtilis. This allows that the part can be used in both kinds.

DATA FOR PARTS THAT WE UPGRADED

  1. Experience - B.subtilis and E.coli Episomal Shuttle Vector with Constitutive RFP to express in E.coli; BBa_K541800: Original forms of BBa_K090401 and BBa_K090402 are upgraded by inserting RFP sequence that can be used for clear characterization and experiments.

DATA FOR PARTS THAT WE HAVE CHARACTERIZED

  1. Promoter Pveg, RBS spoVG and SacB signal peptide for B. Subtilis; (BBa_K541501): This is the subpart of SacB signal peptide which is commonly used in our BioBricks. Signal peptide is used for the secretion of our protein outside of the cell. Promoter and RBS are compatible with B.subtilis.
  2. Promoter Pveg, RBS spoVG and LipA signal peptide for B. Subtilis; (BBa_K541502): Subpart of LipA signal peptide is designed alternatively for BBa_K541501. Promoter and RBS are compatible with B.subtilis.
  3. Constitutive Strong promoter, Strong RBS and TAT signal peptide; (BBa_K541503): The aim of design of this part is same with BBa_K541501 and BBa_K541502. This part is compatible with only E.coli because of its promoter and RBS. Tat signal peptide is inserted in this gene.
  4. IPTG inducible PlacI, RBS and TAT signal peptide; (BBa_K541504): It is same with BBa_K541503. Differently, we used IPTG inducible PlacI promoter with E.coi compatible RBS.
  5. Limulus anti-LPS factor (LALF); (BBa_K541505): LALF subpart that we inserted in our BioBricks which are related to LALF. It has been synthesized in vitro in order to prevent horseshoe crab death, sea creature that hosts LALF. For more information; visit our LALF page.
  6. Reflectin1A from Cephalopod; (BBa_K541506): Reflectin is aimed to use as a biosensor in our project. This is the subpart of parts for reflectin.
2011 © Fatih Medical School

Retrieved from "http://2011.igem.org/Team:Fatih_Turkey/Data_Page"