http://2011.igem.org/wiki/index.php?title=Team:Edinburgh&feed=atom&action=historyTeam:Edinburgh - Revision history2024-03-29T12:40:02ZRevision history for this page on the wikiMediaWiki 1.16.0http://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=246765&oldid=prevL.Kopec: /* Synergy */2011-10-27T10:10:04Z<p><span class="autocomment">Synergy</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We attempted to create microscopic reaction scaffolds holding various enzymes that carry out reactions in the extracellular environment. Our hope is that, by combining the activity of multiple enzymes in a small space, high efficiency will be achieved. Two different systems are being investigated.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We attempted to create microscopic reaction scaffolds holding various enzymes that carry out reactions in the extracellular environment. Our hope is that, by combining the activity of multiple enzymes in a small space, high efficiency will be achieved. Two different systems are being investigated.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Synergy can be assessed <span class="hardword" id="insilico">in silico</span>, using a computer model. We created and compared multiple models of cellulase action and we showed that by just bringing enzymes close together we can increase their efficiency in the following systems.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Cell surface display===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Cell surface display===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These modified phage will be produced by modified ''E. coli''. If multiple copies of the enzymes could be attached to a single phage, then synergy should be achieved. See the [[Team:Edinburgh/Phage Display | phage display]] page for more details.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These modified phage will be produced by modified ''E. coli''. If multiple copies of the enzymes could be attached to a single phage, then synergy should be achieved. See the [[Team:Edinburgh/Phage Display | phage display]] page for more details.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Modelling===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Synergy can be assessed <span class="hardword" id="insilico">in silico</span>, using computer models. We created and compared multiple models of cellulase action and we showed that by just bringing enzymes close together we can increase their efficiency in our potential systems. See [[Team:Edinburgh/Modelling | our modelling page]] for more details.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==DNA assembly==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==DNA assembly==</div></td></tr>
</table>L.Kopechttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=246759&oldid=prevL.Kopec: /* Synergy */ - Modelling2011-10-27T10:08:16Z<p><span class="autocomment">Synergy: </span> - Modelling</p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We attempted to create microscopic reaction scaffolds holding various enzymes that carry out reactions in the extracellular environment. Our hope is that, by combining the activity of multiple enzymes in a small space, high efficiency will be achieved. Two different systems are being investigated.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We attempted to create microscopic reaction scaffolds holding various enzymes that carry out reactions in the extracellular environment. Our hope is that, by combining the activity of multiple enzymes in a small space, high efficiency will be achieved. Two different systems are being investigated.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Synergy can be assessed <span class="hardword" id="insilico">in silico</span>, using a computer model. We created and compared multiple models of cellulase action and we showed that by just bringing enzymes close together we can increase their efficiency in the following systems.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Cell surface display===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Cell surface display===</div></td></tr>
</table>L.Kopechttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=246753&oldid=prevL.Kopec: will -> past tene2011-10-27T10:04:18Z<p>will -> past tene</p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's [[Main Page | 2011 iGEM]] project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. We <del class="diffchange diffchange-inline">will investigate </del>two methods of bringing the enzymes close together: [[Team:Edinburgh/Cell Display | cell surface display]] via Ice Nucleation Protein, and [[Team:Edinburgh/Phage Display | phage display]] via M13's major coat protein. We <del class="diffchange diffchange-inline">will attempt </del>a new DNA assembly protocol, provisionally named "[[Team:Edinburgh/BioSandwich |BioSandwich]]". We <del class="diffchange diffchange-inline">will construct </del>[[Team:Edinburgh/Modelling | computer models]] of synergy. Finally, we <del class="diffchange diffchange-inline">will consider </del>the broader [[Team:Edinburgh/Biorefinery | economic]] and [[Team:Edinburgh/Interviews | social]] questions surrounding the construction of a biorefinery: can it be done, and should it be done?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's [[Main Page | 2011 iGEM]] project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. We <ins class="diffchange diffchange-inline">investigated </ins>two methods of bringing the enzymes close together: [[Team:Edinburgh/Cell Display | cell surface display]] via Ice Nucleation Protein, and [[Team:Edinburgh/Phage Display | phage display]] via M13's major coat protein. We <ins class="diffchange diffchange-inline">attempted </ins>a new DNA assembly protocol, provisionally named "[[Team:Edinburgh/BioSandwich |BioSandwich]]". We <ins class="diffchange diffchange-inline">constructed </ins>[[Team:Edinburgh/Modelling | computer models]] of synergy <ins class="diffchange diffchange-inline">to assess whether it is a feasible option</ins>. Finally, we <ins class="diffchange diffchange-inline"> considered </ins>the broader [[Team:Edinburgh/Biorefinery | economic]] and [[Team:Edinburgh/Interviews | social]] questions surrounding the construction of a biorefinery: can it be done, and should it be done?</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Last year, [https://2010.igem.org/Team:Slovenia Slovenia] found a way to achieve synergy in the <span class="hardword" id="cytoplasm">cytoplasm</span>. This year, Edinburgh is investigating whether such synergy can be achieved outside the cell.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Last year, [https://2010.igem.org/Team:Slovenia Slovenia] found a way to achieve synergy in the <span class="hardword" id="cytoplasm">cytoplasm</span>. This year, Edinburgh is investigating whether such synergy can be achieved outside the cell.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We <del class="diffchange diffchange-inline">will attempt </del>to create microscopic reaction scaffolds holding various enzymes that carry out reactions in the extracellular environment. Our hope is that, by combining the activity of multiple enzymes in a small space, high efficiency will be achieved. Two different systems are being investigated.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We <ins class="diffchange diffchange-inline">attempted </ins>to create microscopic reaction scaffolds holding various enzymes that carry out reactions in the extracellular environment. Our hope is that, by combining the activity of multiple enzymes in a small space, high efficiency will be achieved. Two different systems are being investigated.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Cell surface display===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Cell surface display===</div></td></tr>
</table>L.Kopechttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=157130&oldid=prevFionntom at 18:16, 21 September 20112011-09-21T18:16:32Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Cell surface display===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Cell surface display===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Edinburgh-Surface-Display.png|thumb|300px|''E. coli'' displaying multiple enzymes.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Edinburgh-Surface-Display.png|thumb|300px|''E. coli'' displaying multiple enzymes.]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Edinburgh-Phage-Display.png|thumb|300px|M13 phage displaying multiple enzymes.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Edinburgh-Phage-Display.png|thumb|300px|M13 phage displaying multiple enzymes.]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></div></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The simplest system uses ''E. coli'' bacteria as the scaffold. Each bacterium generates several enzymes and displays them on its outer membrane. If sufficiently high numbers are present on each cell, the synergystic effect should be achieved.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The simplest system uses ''E. coli'' bacteria as the scaffold. Each bacterium generates several enzymes and displays them on its outer membrane. If sufficiently high numbers are present on each cell, the synergystic effect should be achieved.</div></td></tr>
</table>Fionntomhttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=137712&oldid=prevAllancrossman at 17:48, 19 September 20112011-09-19T17:48:28Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's 2011 iGEM project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. We will investigate two methods of bringing the enzymes close together: [[Team:Edinburgh/Cell Display | cell surface display]] via Ice Nucleation Protein, and [[Team:Edinburgh/Phage Display | phage display]] via M13's major coat protein. We will attempt a new DNA assembly protocol, provisionally named "[[Team:Edinburgh/BioSandwich |BioSandwich]]". We will construct [[Team:Edinburgh/Modelling | computer models]] of synergy. Finally, we will consider the broader [[Team:Edinburgh/Biorefinery | economic]] and [[Team:Edinburgh/Interviews | social]] questions surrounding the construction of a biorefinery: can it be done, and should it be done?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's <ins class="diffchange diffchange-inline">[[Main Page | </ins>2011 iGEM<ins class="diffchange diffchange-inline">]] </ins>project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. We will investigate two methods of bringing the enzymes close together: [[Team:Edinburgh/Cell Display | cell surface display]] via Ice Nucleation Protein, and [[Team:Edinburgh/Phage Display | phage display]] via M13's major coat protein. We will attempt a new DNA assembly protocol, provisionally named "[[Team:Edinburgh/BioSandwich |BioSandwich]]". We will construct [[Team:Edinburgh/Modelling | computer models]] of synergy. Finally, we will consider the broader [[Team:Edinburgh/Biorefinery | economic]] and [[Team:Edinburgh/Interviews | social]] questions surrounding the construction of a biorefinery: can it be done, and should it be done?</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td></tr>
</table>Allancrossmanhttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=117036&oldid=prevAllancrossman at 21:21, 13 September 20112011-09-13T21:21:30Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's 2011 iGEM project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. We will investigate two methods of bringing the enzymes close together: [[Team:Edinburgh/Cell Display | cell surface display]] via Ice Nucleation Protein, and [[Team:Edinburgh/Phage Display | phage display]] via M13's major coat protein. We will attempt a new DNA assembly protocol, provisionally named "[[Team:Edinburgh/BioSandwich | BioSandwich]]". We will construct [[Team:Edinburgh/Modelling | computer models]] of synergy. Finally, we will consider the broader [[Team:Edinburgh/Biorefinery | economic]] and [[Team:Edinburgh/Interviews | social]] questions surrounding the construction of a biorefinery: can it be done, and should it be done?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's 2011 iGEM project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. We will investigate two methods of bringing the enzymes close together: [[Team:Edinburgh/Cell Display | cell surface display]] via Ice Nucleation Protein, and [[Team:Edinburgh/Phage Display | phage display]] via M13's major coat protein. We will attempt a new DNA assembly protocol, provisionally named "[[Team:Edinburgh/BioSandwich |BioSandwich]]". We will construct [[Team:Edinburgh/Modelling | computer models]] of synergy. Finally, we will consider the broader [[Team:Edinburgh/Biorefinery | economic]] and [[Team:Edinburgh/Interviews | social]] questions surrounding the construction of a biorefinery: can it be done, and should it be done?</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td></tr>
</table>Allancrossmanhttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=117002&oldid=prevAllancrossman at 21:10, 13 September 20112011-09-13T21:10:44Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's 2011 iGEM project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. We investigate two methods of bringing the enzymes close together: cell surface display via Ice Nucleation Protein, and phage display via M13's major coat protein. We attempt a new DNA assembly protocol, provisionally named "BioSandwich". We construct computer models of synergy. Finally, we consider the broader economic and social questions surrounding the construction of a biorefinery: can it be done, and should it be done?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's 2011 iGEM project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology; and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. We <ins class="diffchange diffchange-inline">will </ins>investigate two methods of bringing the enzymes close together: <ins class="diffchange diffchange-inline">[[Team:Edinburgh/Cell Display | </ins>cell surface display<ins class="diffchange diffchange-inline">]] </ins>via Ice Nucleation Protein, and <ins class="diffchange diffchange-inline">[[Team:Edinburgh/Phage Display | </ins>phage display<ins class="diffchange diffchange-inline">]] </ins>via M13's major coat protein. We <ins class="diffchange diffchange-inline">will </ins>attempt a new DNA assembly protocol, provisionally named "<ins class="diffchange diffchange-inline">[[Team:Edinburgh/</ins>BioSandwich <ins class="diffchange diffchange-inline">| BioSandwich]]</ins>". We <ins class="diffchange diffchange-inline">will </ins>construct <ins class="diffchange diffchange-inline">[[Team:Edinburgh/Modelling | </ins>computer models<ins class="diffchange diffchange-inline">]] </ins>of synergy. Finally, we <ins class="diffchange diffchange-inline">will </ins>consider the broader <ins class="diffchange diffchange-inline">[[Team:Edinburgh/Biorefinery | </ins>economic<ins class="diffchange diffchange-inline">]] </ins>and <ins class="diffchange diffchange-inline">[[Team:Edinburgh/Interviews | </ins>social<ins class="diffchange diffchange-inline">]] </ins>questions surrounding the construction of a biorefinery: can it be done, and should it be done?</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td></tr>
</table>Allancrossmanhttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=116979&oldid=prevAllancrossman at 21:04, 13 September 20112011-09-13T21:04:49Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's 2011 iGEM project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology<del class="diffchange diffchange-inline">, </del>and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A biorefinery is a special type of refinery in which biomass, such as plant <span class="hardword" id="cellulose">cellulose</span>, is converted by microorganisms into useful products. Edinburgh's 2011 iGEM project is a feasibility study into the creation of biorefineries using <span class="hardword" id="ec">E. coli</span>, the workhorse of synthetic biology<ins class="diffchange diffchange-inline">; </ins>and whether biorefineries can be improved by arranging for the different enzymes involved to be in close proximity to each other, so as to create <span class="hardword" id="synergy">synergy</span> between them. <ins class="diffchange diffchange-inline">We investigate two methods of bringing the enzymes close together: cell surface display via Ice Nucleation Protein, and phage display via M13's major coat protein. We attempt a new DNA assembly protocol, provisionally named "BioSandwich". We construct computer models of synergy. Finally, we consider the broader economic and social questions surrounding the construction of a biorefinery: can it be done, and should it be done?</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Synergy==</div></td></tr>
</table>Allancrossmanhttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=113215&oldid=prevAllancrossman: /* Questions */2011-09-12T10:35:47Z<p><span class="autocomment">Questions</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our feasibility study therefore seeks to answer the following questions:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our feasibility study therefore seeks to answer the following questions:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">Is it possible to improve </del>the efficiency of cellulases by displaying them on a cell outer membrane?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">Would </ins>the efficiency of cellulases <ins class="diffchange diffchange-inline">be increased by having different types close together?</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">Is it possible to improve the efficiency of cellulases </del>by displaying them on a phage?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Could this be done </ins>by displaying them on a cell outer membrane?</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">Could this be done </ins>by displaying them on a phage?</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Does the BioSandwich DNA assembly method work properly?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Does the BioSandwich DNA assembly method work properly?</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* How would a biorefinery involving either system actually be constructed?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* How would a biorefinery involving either system actually be constructed?</div></td></tr>
</table>Allancrossmanhttp://2011.igem.org/wiki/index.php?title=Team:Edinburgh&diff=112276&oldid=prevAllancrossman: /* Questions */2011-09-11T19:34:31Z<p><span class="autocomment">Questions</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our feasibility study therefore seeks to answer the following questions:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our feasibility study therefore seeks to answer the following questions:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Does the BioSandwich DNA assembly method work properly?</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Is it possible to improve the efficiency of cellulases by displaying them on a cell outer membrane?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Is it possible to improve the efficiency of cellulases by displaying them on a cell outer membrane?</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Is it possible to improve the efficiency of cellulases by displaying them on a phage?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Is it possible to improve the efficiency of cellulases by displaying them on a phage?</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Does the BioSandwich DNA assembly method work properly?</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* How would a biorefinery involving either system actually be constructed?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* How would a biorefinery involving either system actually be constructed?</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Would such a biorefinery be economically viable?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Would such a biorefinery be economically viable?</div></td></tr>
</table>Allancrossman