Team:ETH Zurich/Project

From 2011.igem.org

Revision as of 12:52, 12 July 2011 by Irenak (Talk | contribs)


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.


You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
ETH Zurich logo.png

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:ETH Zurich team.png
Your team picture
Team Example


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions



Contents

Overall project

Idea

Our project focuses on the detection of smoke from the air by E.Coli bacteria and monitoring its concentration visually by flashes of various lights. Our system will act as a smoke sensor and “partymeter”, telling us how a certain party progresses (under the creative assumption that the progress of the party is measured by the amount of smoke in the air).

Project description

We modify our cells so that they can detect Acetaldehyde, which is a substance found in the cigarete smoke. Upon binding of Acetaldehyde to AlcR, AlcR acts as an inhibitor for a gene transcription. This is where our genetically engineered circuit? / pathway? starts. With a number of inhibitors and activators, we crated our circuit such that it gives rise to a GFP product once a certain concentration of Acetaldehyde is detected from the air. Thus, our circuit acts as a band-pass-filter for Acetaldehyde concentration.

We will place our genetically engineered cells in a microfluidics channel and will supply them with a flow of Acetaldehyde. We will also design our cells to degrade Acetaldehyde, so that a concentration gradient of Acetaldehyde is created throughout the channel.

Project Details

Part 2

The Experiments

Part 3

Results