Team:ETH Zurich/Process/Validation

System validation for diffusion

To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution.

Experimental setup for the diffusion test in agarose filled tubes.

GFP gradient in tube E.coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm.