Team:ETH Zurich/Process/Validation

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Systems Validation
Diffusion-only System A
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System validation for diffusion

Experimental setup for the diffusion test in agarose filled tubes.

To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube (2 mm diameter, 7 cm) long with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution.


GFP gradient in tube E.coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm.