Team:ETH Zurich/Biology/Journal

From 2011.igem.org

(Difference between revisions)
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== ''' Week 3:  4.7-10.7''' ==
== ''' Week 3:  4.7-10.7''' ==
 +
* Working on the design for the system
* Design of several Operons for AlcR and several PCR primer
* Design of several Operons for AlcR and several PCR primer
-
* Making of competent DH5-α cells
 
== '''Week 4:  11.7-17.7''' ==
== '''Week 4:  11.7-17.7''' ==
 +
* Ordering of the LacI<sub>M1</sub> and codonoptimized AlcR gene
 +
* Making of competent DH5-&alpha; cells
* Cloning of the AlcR-testsystem
* Cloning of the AlcR-testsystem
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** P<sub>tet</sub> and CI -had to be redone because of point mutation in the primer (week 9)
** P<sub>tet</sub> and CI -had to be redone because of point mutation in the primer (week 9)
** P<sub>const</sub> and luxR
** P<sub>const</sub> and luxR
-
+
 
 +
* First meeting with Dr. Oliver Frey (Bio engineering laboratory, Prof. Andreas Hierlemann, D-BSSE ETHZ) to exchange ideas about microfluidic design
== '''Week 8:  8.8-14.8''' ==
== '''Week 8:  8.8-14.8''' ==

Revision as of 13:08, 22 August 2011

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Week 1: 20.6-26.6

  • First meeting
  • Brainstorming


Week 2: 27.6-3.7

  • Brainstorming


Week 3: 4.7-10.7

  • Working on the design for the system
  • Design of several Operons for AlcR and several PCR primer

Week 4: 11.7-17.7

  • Ordering of the LacIM1 and codonoptimized AlcR gene
  • Making of competent DH5-α cells
  • Cloning of the AlcR-testsystem

Week 5: 18.7-24.7

  • First test of the AlcR-system in 96-well plates

Week 6: 25.7-31.7

  • Transformation of the parts from the iGEM plates

Week 7: 1.8-7.8

  • Growth test of DH5-α with AlcR-system in flasks
  • Ligation and Transformation of
    • λP and luxI
    • λP and lacI
    • Plac and GFPLVA
    • Plux and mCherry
    • Ptet and CI -had to be redone because of point mutation in the primer (week 9)
    • Pconst and luxR
  • First meeting with Dr. Oliver Frey (Bio engineering laboratory, Prof. Andreas Hierlemann, D-BSSE ETHZ) to exchange ideas about microfluidic design

Week 8: 8.8-14.8

  • Synthesized LacIM1 Gen arrived
  • Ligation and Transformation of
    • Plac-GFPLVA and Terminator
    • Plux-mCherry and Terminator
    • Pconst-luxR and Terminator
    • Plac-GFPLVA-Terminator and Plux-mCherry-Terminator on one plasmid
    • Ptet and LacIM1
    • Ptet-LacIM1 and Terminator


  • Improving of the testsystem
    • Exchange of same rare codons in AlcR by PCR
    • His-tagging of AlcR
    • GFP assam
  • Transformation of λP
  • Making of competent JM101 cells
    • Check for transformation efficiency

Week 9: 15.8-21.8

  • Growth and repression test of AlcR-system with M9-medium
  • Ligation and Transformation of
    • λP and lacI
    • λP and luxI
    • Ptet and CI
    • λP-lacI and terminator
    • λP-luxI and terminator

Week 10: 22.8-28.8