"
Team:ETH Zurich/Biology/Cloning
From 2011.igem.org
(Difference between revisions)
(→Design of a AlcR/acetaldehyde repressed promotor PAlcR) |
|||
| Line 13: | Line 13: | ||
* BBa C0062 LuxR | * BBa C0062 LuxR | ||
* BBa B0015 Double Terminator | * BBa B0015 Double Terminator | ||
| + | |||
| + | |||
| + | |||
| + | == '''Plasmid strategy''' == | ||
| + | |||
| + | http://www.springerlink.com/content/3fj1xxl9p42kx8l5/ | ||
| + | |||
Revision as of 16:10, 18 September 2011
Used parts from registry
- BBa R0040 PTet
- BBa R0051 λP
- BBa R0010 Plac
- BBa R0061 Plux
- BBa J23100 Pconst
- BBa C0061 LuxILVA
- BBa C0040 TetRLVA
- BBa C0051 CI
- BBa C0062 LuxR
- BBa B0015 Double Terminator
Plasmid strategy
http://www.springerlink.com/content/3fj1xxl9p42kx8l5/
Synthesized parts
- LacIM1
- AlcR (codon optimized)
Design of the test-system
To test our designed AlcR-promotors the following system was designed (see Figure). To induce the expression of AlcR a tetracycline induced promotor was used. To monitor the expression of AlcR a His-tag was introduced. In present of acetaldeyhde AlcR binds to the AlcR operon and inhibits the expression of GFP. For a better signal assam gfp was used.
