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Team:ETH Zurich/Achievements/Data page
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Revision as of 23:52, 28 October 2011
Contents |
SmoColi-Data page
SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.
How Our System Works
Our Favorite New Parts
- MainPage-LacIM1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacIM1 binds to Plac and inhibits transcription. (Characterization)
- MainPage-Psb6A5, BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. (Characterization)
- MainPage-PU promoter BBa_K625003; improved version of BBa_I723138 (Glasgow 2007) Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization and Dose response fit)
Characterized New Parts
- MainPage- PTet - LacIM1 - Termination, BBa_K625001 (Characterization)
- MainPage-PU promoter, BBa_K625002; improved version of BBa_I723138 (Glasgow 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)
Characterized Pre-existing Parts
- Experience-PBAD promoter, BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the PBAD in BW27783 (Characterization)
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