Team:EPF-Lausanne

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== Project summary ==
== Project summary ==
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Our goal is to design transcription factors (TFs) that bind to new sequences, currently unavailable to synthetic biologists. These would allow building larger genetic circuits than currently possible, with more independently regulated genes. The new transcription factors will be derived from tetR, one of the better characterised TFs. To characterise them, the tetR mutants and their corresponding mutant promoter are introduced into the circuit illustrated below, containing a LacI inverter and a reporter gene. The reporter for ''in vitro'' experiments is a fluorescence gene such as GFP, and a lysis system for ''in vivo'' experiments. The latter lyses cells with effective transcription factors, so that exclusively their DNA can be recovered.
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Our goal is to design transcription factors (TFs) that bind to new sequences, currently unavailable to synthetic biologists. These would allow to build larger genetic circuits than currently possible, with more independently regulated genes. The new transcription factors will be derived from tetR, one of the better characterised TFs out there. To characterise them, we will introdce the tetR mutants and their corresponding mutant promoters into the circuit illustrated below, containing a LacI inverter and a reporter gene. The reporter for ''in vitro'' experiments is a fluorescence gene such as GFP, and a lysis system for ''in vivo'' experiments. The latter lyses cells with effective transcription factors, so that exclusively their DNA can be recovered.
[[File:EPFL_Summary_(with_TFs).png|700px]]
[[File:EPFL_Summary_(with_TFs).png|700px]]

Revision as of 13:28, 26 July 2011