Team:DTU-Denmark-2/results/Proofofconcept/fungi
From 2011.igem.org
(Difference between revisions)
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<a name="Device BBa_K678060"></a><h2>Device BBa_K678060</h2> | <a name="Device BBa_K678060"></a><h2>Device BBa_K678060</h2> | ||
- | The results shows fluorescence spread evenly in the hyphae which correlate with that in device | + | The results shows fluorescence spread evenly in the hyphae which correlate with that in device BBa_K678060, the gene of interest, only consists of a green fluorescence protein GFP with no specific target. |
<br> | <br> | ||
<br> | <br> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678060 - DIC light.</td> |
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678060 - GFP light.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678060 - Front</td> |
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678060 - Back </td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<a name="Device BBa_K678061"></a><h3>Device BBa_K678061</h3> | <a name="Device BBa_K678061"></a><h3>Device BBa_K678061</h3> | ||
- | The results shows fluorescence spread evenly in the hyphae and with spots of fluorescence. Device | + | The results shows fluorescence spread evenly in the hyphae and with spots of fluorescence. Device BBa_K678061 consists of GFP with a target signal to the peroxisomes which can explain the spots compared to the results from device BBa_K678060. We cannot conclude that the signal is accumulated in the peroxisomes since they are not dyed, though can it be concluded that the GFP signal is target a specific place and accumulated somewhere in the fungi compared to the results from device BBa_K678060. |
<br> | <br> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678061 - DIC light.</td> |
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678061- GFP light.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678061 - Front</td> |
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678061 - Back </td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<a name="Device BBa_K678062"></a><h3>Device BBa_K678062</h3> | <a name="Device BBa_K678062"></a><h3>Device BBa_K678062</h3> | ||
- | The results shows fluorescence spread evenly in the hyphae which correlate with that in device | + | The results shows fluorescence spread evenly in the hyphae which correlate with that in device BBa_K678061, the gene of interest, only consists of a green fluorescence protein RFP with no specific target. |
<br> | <br> | ||
<br> | <br> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678062 - DIC light.</td> |
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678062 - RFP light.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678062- Front</td> |
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678062- Back </td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<a name="Device BBa_K678063"></a><h3>Device BBa_K678063</h3> | <a name="Device BBa_K678063"></a><h3>Device BBa_K678063</h3> | ||
- | The results show spots of fluorescence as in Device | + | The results show spots of fluorescence as in Device BBa_K678061. Device BBa_K678063 consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device BBa_K678060 and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere. |
<br> | <br> | ||
<br> | <br> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678063 - DIC light.</td> |
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678063 - RFP light.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678063 - Front</td> |
- | <td><i>Aspergillus nidulans </i> with device | + | <td><i>Aspergillus nidulans </i> with device BBa_K678063 - Back </td> |
</tr> | </tr> | ||
</table> | </table> |
Revision as of 06:17, 19 September 2011