Team:DTU-Denmark-2

From 2011.igem.org

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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The use of restriction digestion and ligation although developed over 20 years ago, remains to be the standard cloning technique. The use of restriction enzymes can be both a time consuming and cumbersome process. To optimize the cloning process to become faster and more efficient, it is desirable to avoid the use of restriction enzymes.
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Back in 2003 the USER friendly cloning was developed by New England Biolabs to ensure cloning without the use of restriction enzymes. Further development of the method in recent years has meant that construction of the vectors used for USER cloning can now be made quickly and efficiently without the use of restriction enzymes.  
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|You can write a background of your team here. Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:DTU-Denmark-2_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:DTU-Denmark-2 | Team Example]]
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We are developing a standardized system, where certain categories of biological parts can be gathered. The idea is that the parts are in microtiter plates where they can be directly mixed with other desired parts and an expression vector and then can be assembled within a few hours. Hereby purification of digested vectors and PCR-products and ligation are avoided. To show how easy it is, we will demonstrate the use of this system by developing a reporter targeting system for the fungus Aspergillus niger and furthermore demonstrate its application in mammalian cells.
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!align="center"|[[Team:DTU-Denmark-2|Home]]
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{{:Team:DTU-Denmark-2/Templates/Page Footer}}
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!align="center"|[[Team:DTU-Denmark-2/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=DTU-Denmark-2 Official Team Profile]
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!align="center"|[[Team:DTU-Denmark-2/Project|Project]]
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!align="center"|[[Team:DTU-Denmark-2/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:DTU-Denmark-2/Modeling|Modeling]]
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!align="center"|[[Team:DTU-Denmark-2/Notebook|Notebook]]
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!align="center"|[[Team:DTU-Denmark-2/Safety|Safety]]
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!align="center"|[[Team:DTU-Denmark-2/Attributions|Attributions]]
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Revision as of 17:55, 13 July 2011

Team:DTU-Denmark-2/Templates/Page Header

The use of restriction digestion and ligation although developed over 20 years ago, remains to be the standard cloning technique. The use of restriction enzymes can be both a time consuming and cumbersome process. To optimize the cloning process to become faster and more efficient, it is desirable to avoid the use of restriction enzymes. Back in 2003 the USER friendly cloning was developed by New England Biolabs to ensure cloning without the use of restriction enzymes. Further development of the method in recent years has meant that construction of the vectors used for USER cloning can now be made quickly and efficiently without the use of restriction enzymes. We are developing a standardized system, where certain categories of biological parts can be gathered. The idea is that the parts are in microtiter plates where they can be directly mixed with other desired parts and an expression vector and then can be assembled within a few hours. Hereby purification of digested vectors and PCR-products and ligation are avoided. To show how easy it is, we will demonstrate the use of this system by developing a reporter targeting system for the fungus Aspergillus niger and furthermore demonstrate its application in mammalian cells. moreTeam:DTU-Denmark-2/Templates/Page Footer
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