Team:Colombia/Project/Characterization

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The characterization was performed using the same experimental conditions with a fluorometer.
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The strain with the pCPB-mCherry vector was grown in LB media with the antibiotic Kanamycin and the strain with the BBa_J23199 was grown in LB media with Ampicillin.
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The appropriate media with antibiotic was used as blank and for each strain. The intensities were normalized to delete the blank autofluorescence and also the difference between the brightness of each fluorescence protein.
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[[File:Example.jpg]]

Revision as of 03:38, 29 October 2011

Template:Https://2011.igem.org/User:Tabima

The characterization was performed using the same experimental conditions with a fluorometer. The strain with the pCPB-mCherry vector was grown in LB media with the antibiotic Kanamycin and the strain with the BBa_J23199 was grown in LB media with Ampicillin. The appropriate media with antibiotic was used as blank and for each strain. The intensities were normalized to delete the blank autofluorescence and also the difference between the brightness of each fluorescence protein.

Example.jpg