Team:Colombia/Project

From 2011.igem.org

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(Project Details)
(A Brief Approach to our Idea - Un Leve acercamiento a nuestra idea)
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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{|align="justify"
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== '''Overall project''' ==
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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==='''A Brief Approach to our Idea - Un Leve acercamiento a nuestra idea'''===
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|[[Image:Colombia_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Colombia_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Colombia | Team Example]]
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<!--- The Mission, Experiments --->
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---
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'''English:'''
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!align="center"|[[Team:Colombia|Home]]
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!align="center"|[[Team:Colombia/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Colombia Official Team Profile]
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!align="center"|[[Team:Colombia/Project|Project]]
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!align="center"|[[Team:Colombia/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Colombia/Modeling|Modeling]]
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!align="center"|[[Team:Colombia/Notebook|Notebook]]
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!align="center"|[[Team:Colombia/Safety|Safety]]
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!align="center"|[[Team:Colombia/Attributions|Attributions]]
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'''Our objective is to generate a genetically-modified bacteria "detect and alert" system, particularly as a defense aid for coffee plantations against fungi. Bacteria are built such that they can ''detect'' chitin, an organic compound found in fungal cell walls, amplify this initial signal, and finally ''alert'' the plant by stimulating an early hypersensitive response against infection, while controlling their own population density. We strongly believe this biocontrol method may prove to become a powerful tool for farmers and crop workers everywhere. In addition, our constructed "detect and alert" biobricks could be useful to plentyful synthetic biology experiments and future iGEM teams.'''
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Using the bacteria ''Escherichia coli'' DH5α, the system will use two differently-modified bacteria in the system.
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The first bacteria will detect the presence of chitin and will produce chitinase and a signal molecule to engage the response of the second bacteria. The second bacteria will react to the molecule and start to produce a signal (salicylic acid) to activate the hipersensitive response of the plant and control the bacterial populationg growth, since high salicylic acid concetrations are toxic to them.
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[[File:http://i31.photobucket.com/albums/c383/Tabima/Reno/IMG_0666.jpg]]== '''Overall project''' ==
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'''Español:'''
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Your abstract
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'''Nuestro objetivo es el de generar un sistema de "detección y alerta" mediante bacterias genéticamente modificadas, particularmente como control biológico de hongos en plantaciones de café. Se construyen bacterias capaces de ''detectar'' quitina, un componente orgánico presente en las paredes celulares de los hongos, amplificar esta señal inicial y finalmente ''alertar'' a la planta, estimulando así una respuesta hipersensible en contra de la infección y al mismo tiempo controlando su densidad poblacional. Creemos firmemente que este mecanismo de biocontrol puede servir como una herramienta poderosa para el agricultor. Adicionalmente, las partes (bricks) sintetizados pueden ser de utilidad para experimentos en biología sintética y futuros equipos iGEM.'''
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== Project Details==
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Inicialmente las pruebas se realizarán a nivel de laboratorio, empleando como microorganismo modelo a ''Escherichia coli'' DH5α, sin embargo, se espera desarrollar finalmente un producto que pueda ser aplicado en campo.
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Para este fin se emplearán dos bacterias, la primera de las cuales detectará la presencia de quitina y producirá quitinasa y una molécula señalizadora para activar la respuesta de la segunda bacteria, que a su vez regulará la señal de la primera y producirá ácido salicílico para alertar a la planta de la presencia de hongos y controlar la población bacteriana puesto altas concentraciones de ácido salicílico son tóxicas para ellas.
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 03:55, 29 September 2011

Template:Https://2011.igem.org/User:Tabima



Overall project

A Brief Approach to our Idea - Un Leve acercamiento a nuestra idea

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English:

Our objective is to generate a genetically-modified bacteria "detect and alert" system, particularly as a defense aid for coffee plantations against fungi. Bacteria are built such that they can detect chitin, an organic compound found in fungal cell walls, amplify this initial signal, and finally alert the plant by stimulating an early hypersensitive response against infection, while controlling their own population density. We strongly believe this biocontrol method may prove to become a powerful tool for farmers and crop workers everywhere. In addition, our constructed "detect and alert" biobricks could be useful to plentyful synthetic biology experiments and future iGEM teams.

Using the bacteria Escherichia coli DH5α, the system will use two differently-modified bacteria in the system.

The first bacteria will detect the presence of chitin and will produce chitinase and a signal molecule to engage the response of the second bacteria. The second bacteria will react to the molecule and start to produce a signal (salicylic acid) to activate the hipersensitive response of the plant and control the bacterial populationg growth, since high salicylic acid concetrations are toxic to them.


Español:

Nuestro objetivo es el de generar un sistema de "detección y alerta" mediante bacterias genéticamente modificadas, particularmente como control biológico de hongos en plantaciones de café. Se construyen bacterias capaces de detectar quitina, un componente orgánico presente en las paredes celulares de los hongos, amplificar esta señal inicial y finalmente alertar a la planta, estimulando así una respuesta hipersensible en contra de la infección y al mismo tiempo controlando su densidad poblacional. Creemos firmemente que este mecanismo de biocontrol puede servir como una herramienta poderosa para el agricultor. Adicionalmente, las partes (bricks) sintetizados pueden ser de utilidad para experimentos en biología sintética y futuros equipos iGEM.

Inicialmente las pruebas se realizarán a nivel de laboratorio, empleando como microorganismo modelo a Escherichia coli DH5α, sin embargo, se espera desarrollar finalmente un producto que pueda ser aplicado en campo.

Para este fin se emplearán dos bacterias, la primera de las cuales detectará la presencia de quitina y producirá quitinasa y una molécula señalizadora para activar la respuesta de la segunda bacteria, que a su vez regulará la señal de la primera y producirá ácido salicílico para alertar a la planta de la presencia de hongos y controlar la población bacteriana puesto altas concentraciones de ácido salicílico son tóxicas para ellas.