Team:Colombia/Notebook/Protocols

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PCR for 16s (10 µl)

Reactives µl µl
Buffer 2.5 1
MgCl2 2 0.8
DNTP´s 0.5 0.2
P1492R 0.5 0.2
P27F 0.5 0.2
Taq 0.25 0.1
DNA 2 0.8
H2O 17.75 6.8
Final Vol 26 10

PCR Pfx

|Reactives |ul |- |H2O |17.2 |- |Buffer PFX |3 |- |MgSO4 |0.6 |- |dNTP´s |0.9 |- |FW |0.9 |- |RV |0.9 |- |Enhancer |3 |- |Taq PFX |0.5 |- |ADN |3 |- |Total Mix |30