"
Team:Colombia/Notebook/Protocols
From 2011.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
{{ https://2011.igem.org/User:Tabima }} | {{ https://2011.igem.org/User:Tabima }} | ||
| + | |||
| + | ---- | ||
| + | |||
===PCR for 16s (10 µl)=== | ===PCR for 16s (10 µl)=== | ||
{| border="1" align="left" style="text-align:center;" | {| border="1" align="left" style="text-align:center;" | ||
Revision as of 16:29, 27 September 2011
Template:Https://2011.igem.org/User:Tabima
PCR for 16s (10 µl)
| Reactives | µl | µl |
| Buffer | 2.5 | 1 |
| MgCl2 | 2 | 0.8 |
| DNTP´s | 0.5 | 0.2 |
| P1492R | 0.5 | 0.2 |
| P27F | 0.5 | 0.2 |
| Taq | 0.25 | 0.1 |
| DNA | 2 | 0.8 |
| H2O | 17.75 | 6.8 |
| Final Vol | 26 | 10 |
PCR Pfx
| Reactives | ul |
| H2O | 17.2 |
| Buffer PFX | 3 |
| MgSO4 | 0.6 |
| dNTP´s | 0.9 |
| FW | 0.9 |
| RV | 0.9 |
| Enhancer | 3 |
| Taq PFX | 0.5 |
| ADN | 3 |
| Total Mix | 30 |
