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Team:Colombia/Notebook/Plasmids
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Revision as of 18:06, 28 September 2011
Template:Https://2011.igem.org/User:Tabima
Contents |
Plasmids
The Plasmids will be constructed as shown below
Plasmid 1
Plasmid 2
Plasmid 3
In a nutshell, P3’s mission is that of interpreting P2’s autoinduction signals (LuxI) in a way so that it re- sponds with an appropriate production of the plant’s Systemic Aquired Resistance (SAR) stimulating factor (Salicylic Acid, SA). We have also included a negative feedback loop in order to control possible gene overexpressions by in- troducing a LuxI activated lactonase.
Test Plasmid
Building a whole working system is the challenge our group faces everyday. But, as important as the assemblage itself is to test our assumptions about how the designed parts work individually. Currently, this little group comprised by David Ayala-Usma and Óscar Ortega Sandoval with collaboration of Paola Reyes and Nathaly Montenegro, is working on the induction of the Systemic Acquired Resistance of Nicotiana benthamiana (model organism) by Salicylic Acid BioBrick (BBa_J45320) and the response of the designed chitin-induced promoter to (GlcNAc) polymers.
