Team:Cambridge/Protocols/Transformation of B. subtilis

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OVERVIEW
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Protocol Name

Theory

How it works

Practice

  • Add to a clean Eppendorf tube:
Reagent Quantity
competent Bacillus cells 400μl
DNA 3μl
  • Incubate at 37ºC for 1 hour.
  • Plate on an agar plate containing antibiotic, which allows for selection of successful transformants.

Safety

All equipment (including gloves) that may have come into contact with the bacteria must be autoclaved for decontamination purposes.