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Team:Cambridge/Protocols/Transformation of B. subtilis
From 2011.igem.org
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Revision as of 13:20, 18 July 2011
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Protocol Name
Theory
How it works
Practice
- Add to a clean Eppendorf tube:
Reagent Quantity competent Bacillus cells 400μl DNA 3μl
- Incubate at 37ºC for 1 hour.
- Plate on an agar plate containing antibiotic, which allows for selection of successful transformants.
Safety
The safety implication of the procedure.
