"
Team:Cambridge/Protocols/Transformation of B. subtilis
From 2011.igem.org
(Difference between revisions)
(→Practice) |
(→Practice) |
||
| Line 21: | Line 21: | ||
|- | |- | ||
|} | |} | ||
| + | |||
| + | * Incubate at 37ºC for 1 hour. | ||
| + | * Plate on an agar plate containing antibiotic, which allows for selection of successful transformants. | ||
===Safety=== | ===Safety=== | ||
Revision as of 13:17, 18 July 2011
Loading...
Protocol Name
Theory
How it works
Practice
- Add to a clean Eppendorf tube:
Reagent Quantity Bacillus cells 400μl DNA 3μl
- Incubate at 37ºC for 1 hour.
- Plate on an agar plate containing antibiotic, which allows for selection of successful transformants.
Safety
The safety implication of the procedure.
