Team:Cambridge/Protocols/Extraction of cleaner genomic DNA from squid

Extraction of cleaner genomic DNA from squid

As the quick and dirty method to extract genomic DNA failed to produce genomic DNA suitable for PCR, two new protocols are carried out as an attempt to obtain cleaner genomic DNA. This protocol produces clearner preparations and probably somewhat higher yields of genomic DNA.

Theory

How it works

Practice

1. Prepare squid tissue samples and at least 100μl of DNA extraction buffer per tissue sample.

• DNA Extraction Buffer

1. 10mM Tris pH 8.2
2. 10mM EDTA
3. 200 mM NaCl
4. 0.5% SDS
5. 200 µg/ml

2. Incubate at 50

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Safety

The safety implication of the procedure.