Team:Cambridge/Protocols/Confocal Microscopy of Loligo Eye and Mantle Dermis Samples

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Confocal Microscopy of Loligo Eye and Mantle Dermis Samples

Samples were dissected from fresh Loligo vulgaris squid using disposable samples and razor blades. These were mounted on glass microscope slides with PBS and cover slips.

The confocal microscope was set to capture reflected light (the receiver wavelengths encompassed the incident laser light) and incident beams of 405nm, 488nm, 561nm and 633nm were used. Paul Grant optimised the settings and the images were overlaid. (File name - 19711 squid iridescence 11 will recall the settings used)

Theory

How it works

Practice

How to do it in the lab

Safety

The safety implication of the procedure.