Team:Cambridge/Protocols/Colony PCR

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How it works
How it works
===Practice===
===Practice===
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 +
Composition of 50 μl reaction:
 +
{|
 +
|water
 +
|35.7 μl
 +
|-
 +
|10 mM dNTPs
 +
|1 μl
 +
|-
 +
|10× NH4 Buffer
 +
|5 μl
 +
|-
 +
|50mM MgCl2
 +
|1 μl
 +
|-
 +
|forward primer
 +
|2.5 μl
 +
|-
 +
|reverse primer
 +
|2.5 μl
 +
|-
 +
|liquid culture
 +
|1.3 μl
 +
|-
 +
|Taq polymerase
 +
|1 μl
 +
|}
 +
How to do it in the lab
How to do it in the lab

Revision as of 15:55, 29 August 2011

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Colony PCR

protocol description

Theory

How it works

Practice

Composition of 50 μl reaction:

water 35.7 μl
10 mM dNTPs 1 μl
10× NH4 Buffer 5 μl
50mM MgCl2 1 μl
forward primer 2.5 μl
reverse primer 2.5 μl
liquid culture 1.3 μl
Taq polymerase 1 μl

How to do it in the lab

{Standard layout for procedures is to use:

  • <Procedure title - aka what you are doing>
  1. <step 1>
  2. <step 2>
    • <additional notes/important information regarding the previous step>

the text within the < > is what should be written, don't include < > in actual writeup :P

if in doubt see the gel electrophoresis protocol

}

Safety

The safety implication of the procedure.


Retrieved from "http://2011.igem.org/Team:Cambridge/Protocols/Colony_PCR"