Team:Cambridge/Parts

From 2011.igem.org

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(Reflectin A1)
(Reflectin A1 with poly-His tag)
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===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638201 Reflectin A1 with poly-His tag]===
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638201 Reflectin A1 with poly-His tag]===
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In pure form reflectins may be used to create vibrantly coloured thin films and other devices. We used a poly-His affinity column to purify reflectin for this purpose.
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In pure form reflectins may be used to create [[Team:Cambridge/Project/In_Vitro | vibrantly coloured thin films]] and other devices. We used a poly-His affinity column to purify reflectin from cells for this purpose.
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638402 TorA tag]===
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638402 TorA tag]===

Revision as of 20:05, 20 September 2011

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Featured Parts

A thin film composed of Reflectin A1 from Loligo pealeii, purified using a poly-His affinity column

Reflectin A1

Reflectins are a family of proteins which help give cephalopods their amazing camouflage abilities and which can self-assemble into a variety of multilayered structures. We worked with Reflectin A1 from Loligo pealeii. See our background page for more information about reflectins and their role in cephalopod camouflage, and see our registry page for information about our BioBrick submission.

Reflectin A1 with poly-His tag

In pure form reflectins may be used to create vibrantly coloured thin films and other devices. We used a poly-His affinity column to purify reflectin from cells for this purpose.

TorA tag

We submitted a variant of the TorA leader sequence export tag. This TorA leader sequence variant has had been successfully used to export GFP to the periplasm of E.coli as described here. Our tag differs slightly from the TorA tag already in the registry and should be cheaper to obtain from primer synthesis.

Reflectin A1 with N-terminal TorA tag

Reflectin A1 with N-terminal TorA tag and C-terminal sfGFP

...and the rest

A complete list of parts submitted to the Registry by this year's Cambridge team:

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