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Team:Cambridge/Parts
From 2011.igem.org
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==Featured Parts== | ==Featured Parts== | ||
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===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638001 Reflectin A1]=== | ===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638001 Reflectin A1]=== | ||
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===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638201 Reflectin A1 with poly-His tag]=== | ===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638201 Reflectin A1 with poly-His tag]=== | ||
In pure form reflectins may be used to create vibrantly coloured thin films and other devices. We used a poly-His affinity column to purify reflectin for this purpose. | In pure form reflectins may be used to create vibrantly coloured thin films and other devices. We used a poly-His affinity column to purify reflectin for this purpose. | ||
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===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638402 TorA tag]=== | ===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K638402 TorA tag]=== | ||
Revision as of 16:34, 20 September 2011
Contents |
Featured Parts
Reflectin A1
Reflectins are a family of proteins which help give cephalopods their amazing camouflage abilities and which can self-assemble into a variety of multilayered structures. We worked with Reflectin A1 from Loligo pealeii. See our Background page for more information about reflectins and their role in cephalopod camouflage, and see our registry page for information about our BioBrick submission.
Reflectin A1 with poly-His tag
In pure form reflectins may be used to create vibrantly coloured thin films and other devices. We used a poly-His affinity column to purify reflectin for this purpose.
TorA tag
We submitted a variant of the TorA leader sequence export tag. This TorA leader sequence variant has had been successfully used to export GFP to the periplasm of E.coli as described here. Our tag differs slightly from the TorA tag already in the registry.
Reflectin A1 with N-terminal TorA tag
Reflectin A1 with N-terminal TorA tag and C-terminal sfGFP
...and the rest
A complete list of parts submitted to the Registry by this year's Cambridge team:
<groupparts>iGEM011 Cambridge</groupparts>
