Team:Cambridge/Labwork/Protocols

From 2011.igem.org

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(Protocols)
(Protocols)
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*[[Team:Cambridge/Protocols/Transformation_of_E.Coli |Transformation of E.Coli]] : A simple method of transforming competent E.coli with your DNA of choice
*[[Team:Cambridge/Protocols/Transformation_of_E.Coli |Transformation of E.Coli]] : A simple method of transforming competent E.coli with your DNA of choice
*[[Team:Cambridge/Protocols/Transformation_of_B._subtilis | Tranformation of B. subtilis]] : A technique used to introduce foreign DNA into Bacillus cells.  
*[[Team:Cambridge/Protocols/Transformation_of_B._subtilis | Tranformation of B. subtilis]] : A technique used to introduce foreign DNA into Bacillus cells.  
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*[[Team:Cambridge/Protocols/Transformation of E.coli by Electroporation| Transformation of E.coli by Electroporation]]
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*[[Team:Cambridge/Protocols/Transformation of E.coli by Electroporation| Transformation of E.coli by Electroporation]]: A technique for rapidly inserting DNA into cells, typically plasmid DNA, providing the cells are made competent in the correct manner.
'''Bacterial Cultures'''
'''Bacterial Cultures'''

Revision as of 19:21, 30 August 2011

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OVERVIEW
home

Protocols

A list of all protocols developed during the project. To add one, use the section below.

Amplification of DNA

Analysis of DNA

Preparation of DNA Constructs

  • Primer Design : Some general guidelines on how to design successful primers.
  • Gibson Assembly : A relatively new technique of joining multiple DNA strands reliably

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA from Cells

Microscopy

Protein Extraction

  • Buffer Preparation : Methods to prepare the various buffers used to purify his-tagged reflectin from inclusion bodies in E. coli.
  • Inclusion Body Prep : A method to solubilise recombinant reflectin when it has been expressed in inclusion bodies in E. coli.
  • His-Trap Protein Purification : A method to purify reflectin from E. coli lysate using a his-trap column.

Thin Film Preparation

Gel Electrophoresis by SDS PAGE

Adding new Protocols

To add a new protocol, enter the name in the box below and click new to create the new page. You must then return to this page in order to add in a link to the page by copying the code below.

Add a new link on this page with

#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here