Team:Cambridge/Labwork/Protocols

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'''Microscopy'''
'''Microscopy'''
*[[Team:Cambridge/Protocols/Confocal Microscopy of Loligo Eye and Mantle Dermis Samples | Confocal Microscopy]] : A method to visualise reflectins from squid samples.
*[[Team:Cambridge/Protocols/Confocal Microscopy of Loligo Eye and Mantle Dermis Samples | Confocal Microscopy]] : A method to visualise reflectins from squid samples.
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*[[Team:Cambridge/Protocols/Preparing_Slides_With_Agarose_Supplement_To_Form_Microcolonies | Slide Preparation for Confocal Microscopy]] : A method to perpare slides with agarose supplement to form microcolonies.
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*[[Team:Cambridge/Protocols/Preparing_Slides_With_Agarose_Supplement_To_Form_Microcolonies | Slide Preparation for Confocal Microscopy]] : A method of growing a monolayer of bacterial cells on a slide to aid their microscopic viewing.
*[[Team:Cambridge/Protocols/Trypsin | Trypsinisation]] : A method of dispersing cells from tissue, for microscopy.
*[[Team:Cambridge/Protocols/Trypsin | Trypsinisation]] : A method of dispersing cells from tissue, for microscopy.

Revision as of 17:09, 21 September 2011

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OVERVIEW
home

Protocols

A list of all protocols developed during the project. We used this page as a reference throughout the competition.

Amplification of DNA

  • Polymerase Chain Reaction : A method for amplifying a section of DNA.
  • Colony PCR : PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.

Analysis of DNA

Preparation of DNA Constructs

  • Primer Design : Some general guidelines on how to design successful primers.
  • Gibson Assembly : An extremely powerful technique for joining multiple, arbitrary DNA sequences in one step, compatible with standard assembly.

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA

Microscopy

Protein Purification

Thin Film Preparation

Gel Electrophoresis by SDS PAGE