Team:Cambridge/Labwork/Protocols

From 2011.igem.org

(Difference between revisions)
(Protocols)
Line 23: Line 23:
'''Transformation of Bacterial Cells'''
'''Transformation of Bacterial Cells'''
-
*[[Team:Cambridge/Protocols/Making Competent Cells | Making Competent Cells]] : The methods required to make various cells competent
+
*[[Team:Cambridge/Protocols/Making Competent Cells | Making Competent Cells]] : The methods required to make various cells competent.
-
*[[Team:Cambridge/Protocols/Transformation_of_E.Coli |Transformation of E.Coli]] : A simple method of transforming competent E.coli with your DNA of choice
+
*[[Team:Cambridge/Protocols/Transformation_of_E.Coli |Transformation of E.coli]] : A simple method of transforming competent E.coli with your DNA of choice.
-
*[[Team:Cambridge/Protocols/Transformation_of_B._subtilis | Tranformation of B. subtilis]] : A technique used to introduce foreign DNA into Bacillus cells.  
+
*[[Team:Cambridge/Protocols/Transformation_of_B._subtilis | Tranformation of B.subtilis]] : A technique used to introduce foreign DNA into Bacillus cells.  
*[[Team:Cambridge/Protocols/Transformation of E.coli by Electroporation| Transformation of E.coli by Electroporation]]: A technique for rapidly inserting DNA into cells, typically plasmid DNA, providing the cells are made competent in the correct manner.
*[[Team:Cambridge/Protocols/Transformation of E.coli by Electroporation| Transformation of E.coli by Electroporation]]: A technique for rapidly inserting DNA into cells, typically plasmid DNA, providing the cells are made competent in the correct manner.
'''Bacterial Cultures'''
'''Bacterial Cultures'''
-
*[[Team:Cambridge/Protocols/Overnight_Culture |E. coli cell culture]] : A method for growing a cell culture in liquid medium
+
*[[Team:Cambridge/Protocols/Overnight_Culture |E.coli Cell Culture]] : A method for growing a cell culture in liquid medium.
-
*[[Team:Cambridge/Protocols/Glycerol Stocks | Glycerol Stocks]]: A method of storing E.coli cells preserving their viability
+
*[[Team:Cambridge/Protocols/Glycerol Stocks | Glycerol Stocks]]: A method of storing E.coli cells preserving their viability.
'''Extraction of DNA'''
'''Extraction of DNA'''
-
*[[Team:Cambridge/Protocols/Mini_Prep |Mini Prep]] : Extracting DNA from bacterial cells
+
*[[Team:Cambridge/Protocols/Mini_Prep |MiniPrep]] : Extracting DNA from bacterial cells.
-
*[[Team:Cambridge/Protocols/Extraction_of_genomic_DNA_from_squid | Extraction of genomic DNA from squid]] : Two methods to extract genomic DNA from squid tissue
+
*[[Team:Cambridge/Protocols/Extraction_of_Genomic_DNA_from_Squid | Extraction of genomic DNA from squid]] : Two methods to extract genomic DNA from squid tissue.
-
*[[Team:Cambridge/Protocols/Filter_Paper | Extraction of DNA from Filter Paper ]] : Extraction of DNA from filter paper which is a safe way of shipping DNA
+
*[[Team:Cambridge/Protocols/Filter_Paper | Extraction of DNA from Filter Paper ]] : Extraction of DNA from filter paper which is a safe way of shipping DNA.
<!--*[[Team:Cambridge/Protocols/Extraction_of_cleaner_genomic_DNA_from_squid | Extraction of cleaner genomic DNA from squid]] : A method to extract genomic DNA from squid tissue-->
<!--*[[Team:Cambridge/Protocols/Extraction_of_cleaner_genomic_DNA_from_squid | Extraction of cleaner genomic DNA from squid]] : A method to extract genomic DNA from squid tissue-->
Line 49: Line 49:
*[[Team:Cambridge/Protocols/Inclusion_Body_Prep | Inclusion Body Prep]] : Isolation of insoluble inclusion bodies of recombinant proteins.
*[[Team:Cambridge/Protocols/Inclusion_Body_Prep | Inclusion Body Prep]] : Isolation of insoluble inclusion bodies of recombinant proteins.
*[[Team:Cambridge/Protocols/Protein_Purification | His-Trap Protein Purification]] : A method to purify reflectin from E. coli lysate using an affinity column.  
*[[Team:Cambridge/Protocols/Protein_Purification | His-Trap Protein Purification]] : A method to purify reflectin from E. coli lysate using an affinity column.  
-
*[[Team:Cambridge/Protocols/Acetone_Precipitation_of_Proteins | Acetone Precipitation of proteins]] : A method to concentrate solutions of protein.
+
*[[Team:Cambridge/Protocols/Acetone_Precipitation_of_Proteins | Acetone Precipitation of Proteins]] : A method to concentrate solutions of protein.
-
*[[Team:Cambridge/Protocols/Ethanol Precipitation_of_Proteins| Ethanol Precipitation of proteins]] : A method to concentrate solutions of protein.
+
*[[Team:Cambridge/Protocols/Ethanol Precipitation_of_Proteins| Ethanol Precipitation of Proteins]] : A method to concentrate solutions of protein.
-
*[[Team:Cambridge/protocols/Norgen | Norgen Proteospin inclusion body prep.]] : A proprietary kit.
+
*[[Team:Cambridge/protocols/Norgen | Norgen Proteospin Inclusion Body Prep]] : A proprietary kit.
'''Thin Film Preparation'''
'''Thin Film Preparation'''
*[[Team:Cambridge/Protocols/Substrate_Preparation_for_Flow_Coating_and_Spin_Coating | Substrate Preparation]]:How to prepare a substrate for flow coating and spin coating.
*[[Team:Cambridge/Protocols/Substrate_Preparation_for_Flow_Coating_and_Spin_Coating | Substrate Preparation]]:How to prepare a substrate for flow coating and spin coating.
-
*[[Team:Cambridge/Protocols/Spin Coating |Spin Coating to Make a Thin Film]]: Our Spin Coating Protocol
+
*[[Team:Cambridge/Protocols/Spin Coating |Spin Coating to Make a Thin Film]]: Our Spin Coating Protocol.
*[[Team:Cambridge/Protocols/Flow_coating | Flow Coating]]:  How to flow coat a thin film.
*[[Team:Cambridge/Protocols/Flow_coating | Flow Coating]]:  How to flow coat a thin film.
'''Gel Electrophoresis by SDS PAGE'''
'''Gel Electrophoresis by SDS PAGE'''
-
*[[Team:Cambridge/Protocols/Gel_Electrophoresis_of_Protein | Protein Identification by SDS PAGE]]
+
*[[Team:Cambridge/Protocols/Gel_Electrophoresis_of_Protein | Protein Identification by SDS PAGE]]: A method used to separate polypeptides of different lengths.
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}

Revision as of 20:13, 20 September 2011

Loading...
OVERVIEW
home

Protocols

A list of all protocols developed during the project. We used this page as a reference throughout the competition.

Amplification of DNA

  • Polymerase Chain Reaction : A method for amplifying a section of DNA.
  • Colony PCR : PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.

Analysis of DNA

Preparation of DNA Constructs

  • Primer Design : Some general guidelines on how to design successful primers.
  • Gibson Assembly : An extremely powerful technique for joining multiple, arbitrary DNA sequences in one step, compatible with standard assembly.

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA

Microscopy

Protein Purification

Thin Film Preparation

Gel Electrophoresis by SDS PAGE