Team:Cambridge/Labwork/Protocols

From 2011.igem.org

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=Protocols=
=Protocols=
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A list of all protocols developed during the project. To add one, use the section below.
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A list of all protocols developed during the project. We used this page as a reference throughout the competition.
'''Amplification of DNA'''     
'''Amplification of DNA'''     
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'''Gel Electrophoresis by SDS PAGE'''
'''Gel Electrophoresis by SDS PAGE'''
*[[Team:Cambridge/Protocols/Gel_Electrophoresis_of_Protein | Protein Identification by SDS PAGE]]
*[[Team:Cambridge/Protocols/Gel_Electrophoresis_of_Protein | Protein Identification by SDS PAGE]]
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==Adding new Protocols==
 
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To add a new protocol, enter the name in the box below and click new to create the new page. You must then return to this page in order to add in a link to the page by copying the code below.
 
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<html>
 
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  <FORM NAME="NEW_PROT" METHOD="GET" ACTION="HTTP://2011.IGEM.ORG/WIKI/INDEX.PHP" ONSUBMIT="SETTITLE();">#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here
 
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    <p>
 
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      <input type="text" name="title" id="prot_name" value="New Protocol Name"\>
 
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      <input type="hidden" name="action" value="edit"/>
 
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      <input type="hidden" name="preload" value="Template:Team:Cambridge/PROTOCOL"/>
 
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      <input type="Submit" value="New"/>
 
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    </p>
 
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  </form>
 
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  <script type="text/javascript">
 
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    function setTitle()
 
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    {
 
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      var x = document.getElementById('prot_name');
 
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      x.value = 'Team:Cambridge/Protocols/' + x.value.split(' ').join('_');
 
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    }
 
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    function reset()
 
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    {
 
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      document.getElementById('prot_name').value = "New Protocol Name";
 
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    }
 
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  //reset the name if the user goes and uses the back button
 
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  window.onload = reset()
 
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</script>
 
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</html>
 
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Add a new link on this page with
 
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<pre>
 
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#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here
 
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</pre>
 
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}}

Revision as of 20:09, 20 September 2011

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OVERVIEW
home

Protocols

A list of all protocols developed during the project. We used this page as a reference throughout the competition.

Amplification of DNA

  • Polymerase Chain Reaction : A method for amplifying a section of DNA.
  • Colony PCR : PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.

Analysis of DNA

Preparation of DNA Constructs

  • Primer Design : Some general guidelines on how to design successful primers.
  • Gibson Assembly : An extremely powerful technique for joining multiple, arbitrary DNA sequences in one step, compatible with standard assembly.

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA

Microscopy

Protein Purification

Thin Film Preparation

Gel Electrophoresis by SDS PAGE


Retrieved from "http://2011.igem.org/Team:Cambridge/Labwork/Protocols"