Team:Cambridge/Labwork/Protocols

From 2011.igem.org

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A list of all protocols developed during the project. To add one, use the section below.
A list of all protocols developed during the project. To add one, use the section below.
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'''Amplification of DNA'''
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'''Amplification of DNA'''  
*[[Team:Cambridge/Protocols/PCR | Polymerase Chain Reaction]] : A method for amplifying a section of DNA.
*[[Team:Cambridge/Protocols/PCR | Polymerase Chain Reaction]] : A method for amplifying a section of DNA.
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*[[Team:Cambridge/Protocols/Confocal Microscopy of Loligo Eye and Mantle Dermis Samples | Confocal Microscopy]] : A method to visualise reflectins from squid samples.
*[[Team:Cambridge/Protocols/Confocal Microscopy of Loligo Eye and Mantle Dermis Samples | Confocal Microscopy]] : A method to visualise reflectins from squid samples.
*[[Team:Cambridge/Protocols/Preparing_Slides_With_Agarose_Supplement_To_Form_Microcolonies | Slide Preparation for Confocal Microscopy]] : A method to perpare slides with agarose supplement to form microcolonies.
*[[Team:Cambridge/Protocols/Preparing_Slides_With_Agarose_Supplement_To_Form_Microcolonies | Slide Preparation for Confocal Microscopy]] : A method to perpare slides with agarose supplement to form microcolonies.
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#[[Team:Cambridge/Protocols/Trypsinisation |Trypsinisation]] : A method of dispersing cells from tissue, for microscopy.
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*[[Team:Cambridge/Protocols/Trypsinisation |Trypsinisation]] : A method of dispersing cells from tissue, for microscopy.
'''Protein Purification'''
'''Protein Purification'''

Revision as of 14:18, 19 September 2011

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OVERVIEW
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Protocols

A list of all protocols developed during the project. To add one, use the section below.

Amplification of DNA

  • Polymerase Chain Reaction : A method for amplifying a section of DNA.
  • Colony PCR : PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.

Analysis of DNA

Preparation of DNA Constructs

  • Primer Design : Some general guidelines on how to design successful primers.
  • Gibson Assembly : An extremely powerful technique for joining multiple, arbitrary DNA sequences in one step, compatible with standard assembly.

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA

Microscopy

Protein Purification

Thin Film Preparation

Gel Electrophoresis by SDS PAGE

Adding new Protocols

To add a new protocol, enter the name in the box below and click new to create the new page. You must then return to this page in order to add in a link to the page by copying the code below.

#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here

Add a new link on this page with

#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here