Team:Cambridge/Labwork/Protocols

From 2011.igem.org

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(Protocols)
(Protocols)
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'''Protein Purification'''
'''Protein Purification'''
*[[Team:Cambridge/Protocols/Buffers | Buffer Preparation]] : Methods to prepare the various buffers used to purify his-tagged reflectin from inclusion bodies in E. coli.
*[[Team:Cambridge/Protocols/Buffers | Buffer Preparation]] : Methods to prepare the various buffers used to purify his-tagged reflectin from inclusion bodies in E. coli.
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*[[Team:Cambridge/Protocols/Inclusion_Body_Prep | Inclusion Body Prep]] : A method to solubilise recombinant reflectin when it has been expressed in inclusion bodies in E. coli.
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*[[Team:Cambridge/Protocols/Inclusion_Body_Prep | Inclusion Body Prep]] : Isolation of insoluble inclusion bodies of recombinant proteins.
*[[Team:Cambridge/Protocols/Protein_Purification | His-Trap Protein Purification]] : A method to purify reflectin from E. coli lysate using an affinity column.  
*[[Team:Cambridge/Protocols/Protein_Purification | His-Trap Protein Purification]] : A method to purify reflectin from E. coli lysate using an affinity column.  
*[[Team:Cambridge/Protocols/Acetone_Precipitation_of_Proteins | Acetone Precipitation of proteins]] : A method to concentrate solutions of protein.
*[[Team:Cambridge/Protocols/Acetone_Precipitation_of_Proteins | Acetone Precipitation of proteins]] : A method to concentrate solutions of protein.

Revision as of 13:49, 19 September 2011

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OVERVIEW
home

Protocols

A list of all protocols developed during the project. To add one, use the section below.

Amplification of DNA

  • Polymerase Chain Reaction : A method for amplifying a section of DNA.
  • Colony PCR : PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.

Analysis of DNA

Preparation of DNA Constructs

  • Primer Design : Some general guidelines on how to design successful primers.
  • Gibson Assembly : An extremely powerful technique for joining multiple, arbitrary DNA sequences in one step, compatible with standard assembly.

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA

Microscopy

Protein Purification

Thin Film Preparation

Gel Electrophoresis by SDS PAGE

Adding new Protocols

To add a new protocol, enter the name in the box below and click new to create the new page. You must then return to this page in order to add in a link to the page by copying the code below.

Add a new link on this page with

#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here