Team:Cambridge/Labwork/Protocols

From 2011.igem.org

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(Protocols)
(Protocols)
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*[[Team:Cambridge/Protocols/Glycerol Stocks | Glycerol Stocks]]: A method of storing E.coli cells preserving their viability
*[[Team:Cambridge/Protocols/Glycerol Stocks | Glycerol Stocks]]: A method of storing E.coli cells preserving their viability
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'''Extraction of DNA from'''
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'''Extraction of DNA'''
*[[Team:Cambridge/Protocols/Mini_Prep |Mini Prep]] : Extracting DNA from bacterial cells
*[[Team:Cambridge/Protocols/Mini_Prep |Mini Prep]] : Extracting DNA from bacterial cells

Revision as of 16:08, 17 September 2011

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OVERVIEW
home

Protocols

A list of all protocols developed during the project. To add one, use the section below.

Amplification of DNA

  • Polymerase Chain Reaction : A method for amplifying a section of DNA.
  • Colony PCR : PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.

Analysis of DNA

Preparation of DNA Constructs

  • Primer Design : Some general guidelines on how to design successful primers.
  • Gibson Assembly : An extremely powerful technique for joining multiple, arbitrary DNA sequences in one step, compatible with standard assembly.

Transformation of Bacterial Cells

Bacterial Cultures

Extraction of DNA

Microscopy

Protein Extraction

  • Buffer Preparation : Methods to prepare the various buffers used to purify his-tagged reflectin from inclusion bodies in E. coli.
  • Inclusion Body Prep : A method to solubilise recombinant reflectin when it has been expressed in inclusion bodies in E. coli.
  • His-Trap Protein Purification : A method to purify reflectin from E. coli lysate using a his-trap column.

Protein Purification

Thin Film Preparation

Gel Electrophoresis by SDS PAGE

Adding new Protocols

To add a new protocol, enter the name in the box below and click new to create the new page. You must then return to this page in order to add in a link to the page by copying the code below.

Add a new link on this page with

#[[Team:Cambridge/Protocols/PROTOCOL_NAME_HERE |PROTOCOL NAME HERE]] : Insert description here