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Team:Cambridge/Experiments/Squid Dissection and Tissue Sample
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==Squid Dissection and Tissue Samples== | ==Squid Dissection and Tissue Samples== | ||
| - | Specimens of what we identified as [http://www.marlin.ac.uk/speciesinformation.php?speciesID=3718 ''Loligo vulgaris''] and [http://en.wikipedia.org/wiki/Opalescent_Inshore_Squid ''Loligo opalescens''] were dissected for examination. Samples of skin tissue and eye cups were taken from ''L. vulgaris'' for further imaging. No obvious iridescence was seen in the skin sample under a dissection microscope or by confocal microscopy. In accordance with Holt ''et al'', we found clearly visible iridescence in the tissue surrounding the eye lens. | + | Specimens of what we identified as [http://www.marlin.ac.uk/speciesinformation.php?speciesID=3718 ''Loligo vulgaris''] and [http://en.wikipedia.org/wiki/Opalescent_Inshore_Squid ''Loligo opalescens''] were dissected for examination. Samples of skin tissue and eye cups were taken from ''L. vulgaris'' for further imaging. No obvious iridescence was seen in the skin sample under a dissection microscope or by confocal microscopy. In accordance with [http://rsif.royalsocietypublishing.org/content/early/2011/02/14/rsif.2010.0702.full Holt ''et al''], we found clearly visible iridescence in the tissue surrounding the eye lens. |
<gallery> | <gallery> | ||
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File:CAM_L_opalescens.jpg | ''Loligo opalescens'' | File:CAM_L_opalescens.jpg | ''Loligo opalescens'' | ||
File:CAM_L_vulgaris.jpg |''Loligo vulgaris'' specimen dissected | File:CAM_L_vulgaris.jpg |''Loligo vulgaris'' specimen dissected | ||
| + | File:CAM Iridescent.JPG | ''L. vulgaris'' eye cup, showing Bragg reflectance | ||
</gallery> | </gallery> | ||
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | ||
Revision as of 10:02, 21 July 2011
Obtaining DNA coding for reflectin proteins was the vital first step in our project. Loligo tissue was sourced from fishing bait suppliers and culinary wholesalers in order to attempt genomic DNA extraction. From our literature search we discovered that the reflectin gene from E. scolopes contains no introns, so genomic DNA should be suitable for expression in E. coli.
Squid Dissection and Tissue Samples
Specimens of what we identified as Loligo vulgaris and Loligo opalescens were dissected for examination. Samples of skin tissue and eye cups were taken from L. vulgaris for further imaging. No obvious iridescence was seen in the skin sample under a dissection microscope or by confocal microscopy. In accordance with Holt et al, we found clearly visible iridescence in the tissue surrounding the eye lens.
 Preparing squid samples |
 Loligo opalescens |
 Loligo vulgaris specimen dissected |
 L. vulgaris eye cup, showing Bragg reflectance |
