Protein Purification

Bacteria expressing his-tagged reflectin were lysed, and the protein was purified using a his-trap column and a denaturing protocol in order to solubilise reflectin.

Practice

• After high copy expression plasmids for his-tagged reflectin were successfully [assembled] and [transformed] in E. coli, cultures were incubated overnight with () arabinose to induce reflectin expression.
• [Buffers] were prepared, and their pH checked and readjusted if necessary on the day of purification.
• An [inclusion body prep] was performed with 50ml of overnight culture.