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Team:Cambridge/Experiments
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===[[Team:Cambridge/Experiments/Transformation_of_E.coli_with_Plasmids_from_Wendy | Transformation of E.coli with Plasmids from Wendy]]=== | ===[[Team:Cambridge/Experiments/Transformation_of_E.coli_with_Plasmids_from_Wendy | Transformation of E.coli with Plasmids from Wendy]]=== | ||
| + | The first part of the simple procedure involes extraction the plasmid DNA from a piece of paper sent by Wendy, a co-author of a few important papers we cite. The second part is transformation of E.coli with the plasmids DNA to store and amplify the plasmids DNA. | ||
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Revision as of 11:07, 2 August 2011
Contents |
Experiments
Details of the experiments carried out throughout the project are linked from this page. These experiments should also be linked to from the appropriate diary page.
Preliminary Exercise
Initial exercise during our 2 weeks crash course in synthetic biology with the aim of familiarising us with common laboratory methods of preparing and assembling DNA.
Main Project - 'Bactiridescence'
Amplification of Reflectin Genes from the Squid Genomic DNA - Part 1
Reflectin genes were amplified directly from Loligo tissue. Tissue from the Loligo genus was commercially available from fishing bait suppliers and culinary wholesalers. Primers were designed from the nucleotide sequences of three reflectin proteins identified in L. pealei, and used in a PCR reaction upon L. vulgaris genomic DNA.
Amplification of Reflectin Genes from the Squid Genomic DNA - Part 2
Two new protocols for genomic DNA extraction were used in order to improve yield and purity of DNA. In addition to three sets of primers allowing for amplification of reflectin, an extra 'positive control' pair of primers was used in the PCR reaction.
Transformation of E.coli with Plasmids from Wendy
The first part of the simple procedure involes extraction the plasmid DNA from a piece of paper sent by Wendy, a co-author of a few important papers we cite. The second part is transformation of E.coli with the plasmids DNA to store and amplify the plasmids DNA.
