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Team:Cambridge/Experiments
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===Preliminary Exercise=== | ===Preliminary Exercise=== | ||
| - | [[File:CamGFPplasmid.JPG |thumb|400px|right|''fig 1.'' The GFP containing plasmid to which | + | [[File:CamGFPplasmid.JPG |thumb|400px|right|''fig 1.'' The GFP containing plasmid to which the bacillus gene was fused.]] |
Our initial task was to find an interesting protein in Bacillus, and design the appropriate primers to make a GFP fusion to this protein. Then, we used the process of [[Team:Cambridge/Protocols/Gibson_Assembly | Gibson Assembly]] to create our fusion. | Our initial task was to find an interesting protein in Bacillus, and design the appropriate primers to make a GFP fusion to this protein. Then, we used the process of [[Team:Cambridge/Protocols/Gibson_Assembly | Gibson Assembly]] to create our fusion. | ||
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==Main Project - 'Bactiridescence'== | ==Main Project - 'Bactiridescence'== | ||
Reflectin genes were amplified directly from [http://en.wikipedia.org/wiki/Loligo Loligo] tissue. Tissue from the Loligo genus was commercially available from fishing bait suppliers and culinary wholesalers. | Reflectin genes were amplified directly from [http://en.wikipedia.org/wiki/Loligo Loligo] tissue. Tissue from the Loligo genus was commercially available from fishing bait suppliers and culinary wholesalers. | ||
{{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2011_TEMPLATE_FOOT}} | ||
Revision as of 12:33, 19 July 2011
Experiments
Details of the experiments carried out throughout the project are linked from this page. These experiments should also be linked to from the appropriate diary page.
Preliminary Exercise
Our initial task was to find an interesting protein in Bacillus, and design the appropriate primers to make a GFP fusion to this protein. Then, we used the process of Gibson Assembly to create our fusion.
This exercise introduced many of us to laboratory work for the first time, and helped reinforce the key concepts and 'central dogma' as well as practice reading research papers.
The group was divided into three for this exercise; each group worked independently to create a separate gene fusion.
- Cat, Jonathan, Haydn and Ai
- Joe, Matt, Felix and Gerrit
- Katy, Heather, Marta and Veronica
- Positive Control Experiment
Main Project - 'Bactiridescence'
Reflectin genes were amplified directly from Loligo tissue. Tissue from the Loligo genus was commercially available from fishing bait suppliers and culinary wholesalers.
