Team:Calgary

From 2011.igem.org

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<h1 id="pagetitle">Welcome!</h1>
<h1 id="pagetitle">Welcome!</h1>
<img class="boxtop1" src="https://static.igem.org/mediawiki/2011/c/cf/UCalgary_MainPageBox1.png"></img>
<img class="boxtop1" src="https://static.igem.org/mediawiki/2011/c/cf/UCalgary_MainPageBox1.png"></img>
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<p>
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<p>Hello! Our wiki is currently under heavy construction, so please check regularly for updates. Thank you for your patience.<br/>
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The University of Calgary’s iGEM team is working on developing an electrochemical biosensor for Naphthenic Acids (NAs).  NAs are toxic surfactants released into tailings ponds as a by-product of the bitumen extraction process of oil sands. Microorganisms indigenous to tailings ponds that are uniquely capable of degrading NAs suggest that bioremediation may be a viable solution.  To be successful, however, levels of NAs need to be monitored and existing methods for detection are costly and offsite. Using two NA-degrading organisms relatively new to iGEM: microalgae and pseudomonads, we used bioinformatics and a novel NA affinity-based screen in an attempt to identify a sensory element. In the process, we have characterized an electrochemical reporter system and built a working measurement device.  We have also submitted new parts for future work in microalgae, as well as novel parts to move constructs between Pseudomonas and E. coli.  
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The University of Calgary iGEM team aims to build a biosensor for naphthenic acids. Naphthenic acids are a family of organic acids that are toxic to terrestrial and aquatic wildlife, and are waste products of the bitumen extraction process. Naphthenic acids are stored in on-site settling ponds called tailing ponds; not only do they poison the environment, but they also contribute to the corrosion of refinery equipment, which directly increases maintenance and replacement costs. Currently, sophisticated and expensive procedures such as mass spectroscopy and gas chromatography are needed to identify and assess the concentration of naphthenic acids in solution. A bacterial sensor, if developed, could greatly improve the cost- and time-efficiency of naphthenic acid detection, and facilitate a workable approach for remediation.
</p>
</p>
<img class="boxbottom1" src="https://static.igem.org/mediawiki/2011/1/11/UCalgary_MainPageBox1Bottom.png"></img>
<img class="boxbottom1" src="https://static.igem.org/mediawiki/2011/1/11/UCalgary_MainPageBox1Bottom.png"></img>
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<h2>Building a Naphthenic Acid Biosensor</h2>
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<h2>The Vision: A Naphthenic Acid Biosensor</h2>
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<p>This project is separated into four subcomponents:</p>
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<p>We plan to create a relatively small, cheap biosensing device that can respond to naphthenic acids and produce an electrochemical output. Roll over the individual parts for more details.</p>
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<p>The first is an examination of the native tailings pond genus <i>Pseudomonas</i> using ChIP-Seq, and attempting to find a naphthenic acid-sensitive promoter which can then be assembled with a reporter system.
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The second component involves constructing and characterizing an electrochemical reporter system that can produce quantifiable output without the need for optical measurements. This way, even cloudy tailings pond water can yield useful results.
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<img id="sensorimage" src="https://static.igem.org/mediawiki/2011/f/f8/UCalgary2011_MainPageDevice.png"></img>
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At the same time, we are also examining another potential chassis to detect and report naphthenic acids, in the microalgae <i>Chlamydomonas</i>.
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<img id="pulloutbox" src="https://static.igem.org/mediawiki/2011/b/b4/UCalgary2011_MainPageBacterialZoomBox.png"></img>
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Finally, a software tool is currently in development that we hope will be able to locate and report degrees of non-homology between two separate genomes.</p>
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<img id="circuit" src="https://static.igem.org/mediawiki/2011/8/80/UCalgary2011_BacterialCircuitBase.png"></img>
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<a id="promoter" href="https://2011.igem.org/Team:Calgary/Project/Promoter">Promoter</a>
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<p>More details on the biosafety of our project can be found on our <a href="https://2011.igem.org/Team:Calgary/Safety">safety page</a>.</p>
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<a id="reporter" href="https://2011.igem.org/Team:Calgary/Project/Reporter">Reporter</a>
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<a id="chassis" href="https://2011.igem.org/Team:Calgary/Project/Chassis">Chassis</a>
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</div>

Revision as of 08:14, 27 September 2011

Welcome!

Hello! Our wiki is currently under heavy construction, so please check regularly for updates. Thank you for your patience.
The University of Calgary iGEM team aims to build a biosensor for naphthenic acids. Naphthenic acids are a family of organic acids that are toxic to terrestrial and aquatic wildlife, and are waste products of the bitumen extraction process. Naphthenic acids are stored in on-site settling ponds called tailing ponds; not only do they poison the environment, but they also contribute to the corrosion of refinery equipment, which directly increases maintenance and replacement costs. Currently, sophisticated and expensive procedures such as mass spectroscopy and gas chromatography are needed to identify and assess the concentration of naphthenic acids in solution. A bacterial sensor, if developed, could greatly improve the cost- and time-efficiency of naphthenic acid detection, and facilitate a workable approach for remediation.

The Vision: A Naphthenic Acid Biosensor

We plan to create a relatively small, cheap biosensing device that can respond to naphthenic acids and produce an electrochemical output. Roll over the individual parts for more details.

Promoter Reporter Chassis

Our Sponsors

Other Things

Here is a placeholder spot.

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