Team:CBNU-Korea/contenttest3

From 2011.igem.org

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body{
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#footer-box{
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#test{
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margin-right:auto;
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margin-left:auto;
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line-height:30px;
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#logo{
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<img src="https://static.igem.org/mediawiki/2011/1/19/Synb_Project_Description.png" width="524" height="55">
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<img src="https://static.igem.org/mediawiki/2011/c/c1/Synb_Project_Abstract.png">
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<div id="test" style="width:550px; height:500px; background:transparent; overflow:auto;">
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<div id="test" style="width:700px; height:450px; background:transparent; overflow:auto;">
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<br><font color=white>
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<br><font color=white size="4">
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Synthetic minimal genome is the smallest possible group of genes that would be sufficient to sustain a cellular life form under the most favorable conditions imaginable that working like natural chromosome, bacteria have.
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For design the Synthetic Minimal Chromosome, we need to get information of essential genes such as direction, position, length and function in order to design chromosome.
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For synthesizing minimal genome, there are many things that we have to consider such as kind of genes, gene arrangement, direction of genes, codon usage, genome structure, stability and so on. Therefore it is very difficult to synthesize minimal genome considering ALL of these.
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However, it is difficult to design to use only information of these. So, we should have a new analyzing method which calculates distance between replication origin and each essential gene(DTO; Distance to origin) in each specie and get the number of essential genes within 10 percent of total genome size. Using this method, we confirmed a distribution of essential genes in each organism
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So we synthesize simple minimal chromosome employing V.cholerae O1 biovar eltor str. N16961chromosome II replication and partition system as the first step of synthetic minimal genome.
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The information of essential genes is obtained from DEG(Database of Essential Genes) and re-group essential genes by COG distribution for construction our database which is connected to a software named GOD, Genome Organization Database & Designer.
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Also, we re-build essential gene database from DEG and NCBI. And using this database, we build genome designer which is based on essential gene database that is produced by sequential analysis process.
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<a href="javascript:history.go(-1);"><img src="https://static.igem.org/mediawiki/2011/7/77/Synb_back.png"></a>
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Latest revision as of 07:15, 27 September 2011


For design the Synthetic Minimal Chromosome, we need to get information of essential genes such as direction, position, length and function in order to design chromosome. However, it is difficult to design to use only information of these. So, we should have a new analyzing method which calculates distance between replication origin and each essential gene(DTO; Distance to origin) in each specie and get the number of essential genes within 10 percent of total genome size. Using this method, we confirmed a distribution of essential genes in each organism The information of essential genes is obtained from DEG(Database of Essential Genes) and re-group essential genes by COG distribution for construction our database which is connected to a software named GOD, Genome Organization Database & Designer.