Team:Brown-Stanford/PowerCell/Methods

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Brown-Stanford
iGEM

Methods and Materials

Cyanobacterial cultures: we used wild type strains of Anabaena PCC7120, Synechocystis PCC6803, Nostoc punctiforme ATCC 29133, and Synechococcus elongatus PCC 7942, all courtesy of James Golden. These were raised at 30˚C with shaking at 121rpm and ~40µEinsteins of light in liquid BG11 media1.

Brown-Stanford AnabaenaVegetativeCellSecretionDevice.jpg


1 Rippka, R., J. Deruelles, J. B Waterbury, M. Herdman, and R. Y Stanier. 1979. “Generic assignments, strain histories and properties of pure cultures of cyanobacteria.” Journal of General Microbiology 111 (1): 1.