Team:Brown-Stanford/PowerCell/Methods

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== '''Methods and Materials''' ==
== '''Methods and Materials''' ==
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Before bringing synthetic organisms to do work on Mars, one needs to answer the question of what to feed the cultures. Bringing media and nutrients from the Earth to supply large-scale bioreactors is slow and inefficient. PowerCell is a cellular energy generator engineered with blue-green algae (cyanobacteria) to fix atmospheric N2 and CO2 and export them to other cells.  When grown alongside synthetic Escherichia coli, these highly compatible “power plant” cells will fuel the generation of useful products such as medicine, heat, food, and other necessities. Our project aims to prove that cyanobacteria co-cultured with E. coli can become a self-sustaining system.
 
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[[File:Brown-Stanford InvA.png|center|400px]]
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'''Cyanobacterial cultures''': we used wild type strains of Anabaena PCC7120, Synechocystis PCC6803, Nostoc punctiforme ATCC 29133, and Synechococcus elongatus PCC 7942, all courtesy of James Golden.  These were raised at 30˚C with shaking at 121rpm and ~40µEinsteins of light in liquid BG11 media{{:Team:Brown-Stanford/Templates/FootnoteNumber|1}}. 
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[[File:Brown-Stanford AnabaenaVegetativeCellSecretionDevice.jpg|center|700px]]
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[[File:Brown-Stanford AnabaenaVegetativeCellReporterDevice.jpg|center|700px]]
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[[File:Brown-Stanford AnabaenaVegetativeCellSecretionReporterDevice.jpg|center|700px]]
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{{:Team:Brown-Stanford/Templates/Footnote|1|Rippka, R., J. Deruelles, J. B Waterbury, M. Herdman, and R. Y Stanier. 1979. “Generic assignments, strain histories and properties of pure cultures of cyanobacteria.” Journal of General Microbiology 111 (1): 1.}}

Latest revision as of 19:03, 25 August 2011

Brown-Stanford
iGEM

Methods and Materials

Cyanobacterial cultures: we used wild type strains of Anabaena PCC7120, Synechocystis PCC6803, Nostoc punctiforme ATCC 29133, and Synechococcus elongatus PCC 7942, all courtesy of James Golden. These were raised at 30˚C with shaking at 121rpm and ~40µEinsteins of light in liquid BG11 media1.

Brown-Stanford AnabaenaVegetativeCellSecretionDevice.jpg


Brown-Stanford AnabaenaVegetativeCellReporterDevice.jpg


Brown-Stanford AnabaenaVegetativeCellSecretionReporterDevice.jpg


1 Rippka, R., J. Deruelles, J. B Waterbury, M. Herdman, and R. Y Stanier. 1979. “Generic assignments, strain histories and properties of pure cultures of cyanobacteria.” Journal of General Microbiology 111 (1): 1.

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