Team:Brown-Stanford/Lab/Protocols/Nutrient

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(Created page with "{{:Team:Brown-Stanford/Templates/Main}} == '''Nutrient agar with urea''' == #0.5% Peptone #0.3% beef extract/yeast extract #1.5% agar #0.5% NaCl #distilled water #pH adjusted to...")
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== '''Nutrient agar with urea''' ==
== '''Nutrient agar with urea''' ==
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#0.5% Peptone
+
*0.5% Peptone
-
#0.3% beef extract/yeast extract
+
*0.3% beef extract/yeast extract
-
#1.5% agar
+
*1.5% agar
-
#0.5% NaCl
+
*0.5% NaCl
-
#distilled water
+
*distilled water
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#pH adjusted to neutral (6.8)at 25 °C.
+
*pH adjusted to neutral (6.8)at 25 °C.
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#8% urea that is filter sterilized, added after the rest of the stuff is autoclaved
+
*8% urea that is filter sterilized, added after the rest of the stuff is autoclaved
{{:Team:Brown-Stanford/Templates/Foot}}
{{:Team:Brown-Stanford/Templates/Foot}}

Revision as of 23:19, 11 June 2011

Brown-Stanford
iGEM

Nutrient agar with urea

  • 0.5% Peptone
  • 0.3% beef extract/yeast extract
  • 1.5% agar
  • 0.5% NaCl
  • distilled water
  • pH adjusted to neutral (6.8)at 25 °C.
  • 8% urea that is filter sterilized, added after the rest of the stuff is autoclaved