Team:Brown-Stanford/Lab/Protocols/CementationColumn

From 2011.igem.org

(Difference between revisions)
(Created page with "== '''Biocementation Column''' == === '''Materials''' === *60 mL falcon tube *30 g of Lunar/Martian regolith per tube *A peristaltic pump *Two feet of rubber tubing, epoxy gl...")
 
(3 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:Brown-Stanford/Templates/Protocol}}
 +
== '''Biocementation Column''' ==
== '''Biocementation Column''' ==
=== '''Materials''' ===
=== '''Materials''' ===
-
*60 mL falcon tube  
+
*15 mL falcon tube  
-
*30 g of Lunar/Martian regolith per tube
+
*20 g of Lunar/Martian regolith per tube
*A peristaltic pump  
*A peristaltic pump  
*Two feet of rubber tubing, epoxy glue, fabric matting
*Two feet of rubber tubing, epoxy glue, fabric matting
*Heat Lamp
*Heat Lamp
-
*50 mL of S. pasteurii culture (OD600 1.2)
+
*30 mL of S. pasteurii culture (OD600 1.2)
=== '''Procedure''' ===
=== '''Procedure''' ===

Latest revision as of 18:17, 28 September 2011

Brown-Stanford
iGEM

Biocementation Column

Materials

  • 15 mL falcon tube
  • 20 g of Lunar/Martian regolith per tube
  • A peristaltic pump
  • Two feet of rubber tubing, epoxy glue, fabric matting
  • Heat Lamp
  • 30 mL of S. pasteurii culture (OD600 1.2)

Procedure

  • The columns were first prewet with 20 mL of sterile Bang media.
  • The bacteria culture was draw into the feed tube, and allowed to settle in the column overnight
  • Cementation solution (1.5 M equimolar urea and calcium chloride) was fed into the feed tube, which was then placed into a constant loop with the output tube
  • The column was heated for 5 days, and allowed to cycle.