Team:British Columbia/Notebook/Week 7

From 2011.igem.org

Gurpal was working with the erg20 gene and trying to synthesize the erg20-2 mutant from it on July 19th. After ordering and receiving primers (forward and backward), his goal today was to isolate the erg20 gene from wildtype yeast. Three tubes were prepared for the PCR, each containing a different amount of yeast genome, and the PRC was initiated at 2:20pm. When the PRC was finished, the tubes were incubated in a 37 degree incubator for 1.5 hours. Afterwards, Jacob ran a gel electrophoresis experiment to determine if the site was cleaved. However, the gel showed that nothing except our ladder. Therefore, this experiment failed.