Team:Bilkent UNAM Turkey/Collaboration

From 2011.igem.org

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https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Collaboration
https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Collaboration
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'''FATIH_TURKEY'''
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FATIH_TURKEY
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We helped the FATIH_TURKEY team by providing them with B.Subtilis and competent B.Subtilis which was needed in order for their project to proceed. We also gave materials such as LB, antibiotic and agar to accelarate their experiments before their orders come from companies whenever they need. This strain was obtained from one of lab members and prepared to be sent.
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We have assisted the FATIH_TURKEY ([https://2011.igem.org/Team:Fatih_Turkey]) team by providing them with both
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They also gave us their biobrick vectors for ''B. subtilis'':
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unmodified ''B.subtilis'' and competent ''B.subtilis'' cultures, which they
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- BBa_K541026 (Reflectin 1A (SacB) and LALF (LipA) protein for B.subtilis)
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required in order for their project to proceed. We also donated materials
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- BBa_K090401 ( Gram-positive Shuttle Vector for Episomal Expression)
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such as LB, antibiotic and agar to this team so that they may conduct
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- BBa_K090402 (B. subtilis Episomal Vector with Constitutive GFP)
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their preliminary experiments before they obtained the necessary reagents
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- BBa_K090403 (Gram-positive Shuttle Vector for Chromosomal Integration)
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(even though they were diligent in purchasing the needed chemicals
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immediately, orders may take up to 4 months to be delivered in Turkey, a
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We planned to use these vectors for ''B. subtilis'' project but these vectors could not be used because we have not much time to work both ''C. reinhardtii'' and ''B. subtilis'' projects. We continue with ''C. reinhardtii''.
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fact we find most unfortunate.)'' B.subtilis'' cultures were kindly donated to
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the iGEM team from one of lab members (Alper Ozkan) and delivered by hand
 +
to a Fatih iGEM member.
 +
We must also thank them for providing us with biobrick vectors for ''B.
 +
subtilis''. The vectors we have obtained from their team are BBa_K541026
 +
(Reflectin 1A (SacB) and LALF (LipA) protein for ''B.subtilis''), BBa_K090401
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(Gram-positive Shuttle Vector for Episomal Expression), BBa_K090402 (''B.
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subtilis'' Episomal Vector with Constitutive GFP) and BBa_K090403
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(Gram-positive Shuttle Vector for Chromosomal Integration)
 +
We planned to use these vectors for a project concerning the expression of
 +
mussel adhesion foot proteins ''B. subtilis'', but did not get the chance to
 +
utilize them because our relatively small team scarcely had the time to
 +
work with both ''C. reinhardtii'' and ''B. subtilis''. We have opted to continue
 +
with ''C. reinhardtii'', of which preliminary tests yielded promising results.

Revision as of 22:44, 21 September 2011

https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Collaboration FATIH_TURKEY We have assisted the FATIH_TURKEY ([https://2011.igem.org/Team:Fatih_Turkey]) team by providing them with both unmodified ''B.subtilis'' and competent ''B.subtilis'' cultures, which they required in order for their project to proceed. We also donated materials such as LB, antibiotic and agar to this team so that they may conduct their preliminary experiments before they obtained the necessary reagents (even though they were diligent in purchasing the needed chemicals immediately, orders may take up to 4 months to be delivered in Turkey, a fact we find most unfortunate.)'' B.subtilis'' cultures were kindly donated to the iGEM team from one of lab members (Alper Ozkan) and delivered by hand to a Fatih iGEM member. We must also thank them for providing us with biobrick vectors for ''B. subtilis''. The vectors we have obtained from their team are BBa_K541026 (Reflectin 1A (SacB) and LALF (LipA) protein for ''B.subtilis''), BBa_K090401 (Gram-positive Shuttle Vector for Episomal Expression), BBa_K090402 (''B. subtilis'' Episomal Vector with Constitutive GFP) and BBa_K090403 (Gram-positive Shuttle Vector for Chromosomal Integration) We planned to use these vectors for a project concerning the expression of mussel adhesion foot proteins ''B. subtilis'', but did not get the chance to utilize them because our relatively small team scarcely had the time to work with both ''C. reinhardtii'' and ''B. subtilis''. We have opted to continue with ''C. reinhardtii'', of which preliminary tests yielded promising results.
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