Team:Bielefeld-Germany/Results/S-Layer/Guide/5

From 2011.igem.org

(Difference between revisions)
Line 3: Line 3:
=Filtration of the cell lysate=
=Filtration of the cell lysate=
-
[[Image:IGEM-Bielefeld2011-Pump.JPG|400px|thumb|center|A Milipore Pellicon XL 300 membrane actuated with a SciLog TANDEM 1081 peristaltic pump.]]
+
[[Image:IGEM-Bielefeld2011-Pump.JPG|400px|thumb|right|A Milipore Pellicon XL 300 membrane actuated with a SciLog TANDEM 1081 peristaltic pump.]]
Centrifugation does not remove all of the cell debris, therefore a tangential flow ultrafiltration step with a 300 kDa membrane is performed. The retentate is collected. Instead of this device you can also use a simple dead-end sterile filter (0.22 µm poresize) but it is easier the way described above (the dead-end filter gets clogged after some time...).  
Centrifugation does not remove all of the cell debris, therefore a tangential flow ultrafiltration step with a 300 kDa membrane is performed. The retentate is collected. Instead of this device you can also use a simple dead-end sterile filter (0.22 µm poresize) but it is easier the way described above (the dead-end filter gets clogged after some time...).  

Revision as of 01:35, 29 October 2011

Filtration of the cell lysate

A Milipore Pellicon XL 300 membrane actuated with a SciLog TANDEM 1081 peristaltic pump.

Centrifugation does not remove all of the cell debris, therefore a tangential flow ultrafiltration step with a 300 kDa membrane is performed. The retentate is collected. Instead of this device you can also use a simple dead-end sterile filter (0.22 µm poresize) but it is easier the way described above (the dead-end filter gets clogged after some time...).

With the cleared lysate the histidine affinity tag comes into play - want to know how?

Jump to intersection Return to beginning Next page