Team:Arizona State/Templates/E coli contingency/table 4

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Transformation Efficiency Results
E. Coli Strain Initial Plasmid Introduced Plasmid Antibiotic selection DNA Quantity Used (ng) # of Colonies Weighted Colony Count**
MG1655 GFP Construct in pRSF Leader in pIDT Amp 140 21 138.742
MG1655 GFP Construct in pRSF Leader+Seq1 in pIDT Amp 147 31 204.810
MG1655 Leader in pIDT GFP Construct in pRSF Kan 143.4 23 117.635
MG1655 Leader+Seq1 in pIDT GFP Construct in pRSF Kan 143.4 1000 3909.508
BL21 GFP Construct in pRSF Leader in pIDT Amp 140 0 0.000
BL21 GFP Construct in pRSF Leader+Seq1 in pIDT Amp 147 2 11.574
BL21 Leader in pIDT GFP Construct in pRSF Kan 143.4 14 60.512
BL21 Leader+Seq1 in pIDT GFP Construct in pRSF Kan 143.4 9 35.186

**Weighted Colony Count = (# of Colonies)/(mean OD600 of competency prep)