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File:7 5 11 RD VYKJ labeled.jpg 1,327×996 (43,807 bytes) - 15:17, 8 July 2011File:7 5 11 RD VYKJ labeled.jpeg 1,327×996 (43,807 bytes) - 15:18, 8 July 2011File:Week 5..png 195×43 (1,154 bytes) - 14:56, 15 August 2011File:CassettePCR 7-5 (Ura) copy.jpg 1,280×960 (606,727 bytes) - 15:13, 25 July 2011File:20110719 Pxyl+odspB+rsaA re-do c1,3,4,5,6 std.jpg 622×1,020 (47,970 bytes) - 14:09, 5 August 2011File:20110722 BBa+odspB+rsaA+pMR10 c1,5-9.jpg 355×306 (5,033 bytes) - 16:27, 25 July 2011File:Freiburg 11 8 2 2011 5 41 23 PM.jpg 551×371 (93,003 bytes) - 11:18, 3 August 2011File:8-5-11-1.JPG 760×574 (32,093 bytes) - 21:43, 5 August 2011File:8-5-11-2.JPG 760×574 (43,268 bytes) - 21:55, 5 August 2011File:8-5-11-3.JPG 760×574 (32,661 bytes) - 03:12, 6 August 2011File:Super BB minus p1 1-5-2.jpg 433×398 (76,632 bytes) - 07:36, 24 August 2011File:5-.jpg 131×33 (1,892 bytes) - 00:11, 28 August 2011File:5+.jpg 131×33 (3,013 bytes) - 00:11, 28 August 2011File:S79 5(2).jpg 608×627 (576,994 bytes) - 10:03, 2 September 2011File:UP AG 1,5% 2011-08-10 JE 002.jpg 596×449 (144,598 bytes) - 09:12, 5 September 2011File:UP AG 1,5% digest NgoMIV iGEM TEV Protease iGEM BamHI 20110810.jpg 449×449 (125,864 bytes) - 09:12, 5 September 2011File:Interview 5 image.jpg 209×141 (8,174 bytes) - 19:00, 20 September 2011File:5-Human-Practice.png 393×34 (2,526 bytes) - 16:08, 29 September 2011File:5 Aug Results pict.JPG 1,701×648 (232,813 bytes) - 05:02, 14 September 2011File:Nid 5 back.jpg 2,313×2,135 (764,893 bytes) - 23:13, 15 September 2011
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File:20110628 TestDigest.jpg ...SpeI; lane 6: cut with PstI; lane 7: standard uncut DNA fragment for lanes 5 and 6. There is an apparent decrease in size from standard DNA fragments t754×810 (18,449 bytes) - 14:29, 29 June 2011File:20110706 MoreColonies.jpg Lane 1: ladder; lanes 2-5: colonies that should contain <i>rsaA</i> inserted into pSB1C3 containing < Lanes 5 and 6 show odd results that could be from incomplete digest or successful i832×552 (25,851 bytes) - 15:17, 7 July 2011File:20110706 MoreColoniesFollowup.jpg Follow up gel of lanes 5 and 6 from the previous gel, redigested for a longer period of time. ...t of <i>rsaA</i> into plasmid already containing <i>esp</i> (previous lane 5); lane 3: digest of miniprep product from colony that should contain an ins330×754 (10,054 bytes) - 15:22, 7 July 2011File:20110709dspB+pxyl Colonies5-8.jpg ... with Pxyl colonies. Lane 1: ladder; lanes 2-5: WT dspB with Pxyl colonies 5-8; lane 6: standard dspB with no promoter.311×385 (5,663 bytes) - 14:03, 5 August 2011File:20110710 optGenes+C3.jpg ...timized dspB transformed into pSB1C3 colonies A-E. Lane 1: ladder; lanes 2-5: optimized dspB colonies A-E; lanes 6-9: optimized esp. Colony D shows the450×440 (8,008 bytes) - 14:04, 5 August 2011File:20110714 optdspB+rsaA+Pxyl c5-8.jpg Figure 10: Colonies 5-8 of Pxyl + dspB + rsaA. Lane1: ladder; lanes 2-5: carry Pxyl + optimized dspB + rsaA; lane 6: standard optimized dspB with r322×371 (5,815 bytes) - 14:07, 5 August 2011File:20110718 optespC3 optespPxylC3.jpg ...mants that should carry optimized <i>esp</i> behind P<sub>xyl</sub>. Lanes 5 and 7 have the desired gene sequence.894×670 (23,076 bytes) - 21:47, 19 July 2011File:20110614 PCR135.jpg ...aining ''esp'' ligated with ''rsaA'' that had been digested 20110606; lane 5: PCR product of plasmid containing ''esp''; lanes 6-8: PCR product using VF834×1,018 (23,175 bytes) - 19:36, 16 June 2011File:20110617 Pro-esp-stop-rsaA.jpg ...: ladder; lanes 2-4 and 6-8: PCR product of transformation survivors; lane 5: control DNA of ''esp'' + ''rsaA''.798×930 (20,227 bytes) - 21:03, 26 June 2011File:20110627 esprsaA+promoters 1.jpg ...insert into plasmids containing various promoters. Lane 1: ladder; lanes 2-5: inserts into plasmid containing BBa_K081005; lane 6: standard BBa_K0810051,062×574 (14,974 bytes) - 15:32, 28 June 2011File:1st Micro Congress.pdf In order to meet the 5 Mexican teams and talk about common problems that have arisen along the com(1,590,816 bytes) - 22:55, 20 June 2011File:20110620 promotorsTransformationGel.jpg ...of two small bands shows contamination of samples. Lane 1: ladder; lanes 2-5: plasmid PCR of transformation survivors.408×834 (11,552 bytes) - 20:54, 22 June 2011File:20110621 Promoter-esp-Stop-rsaA-pMR10.jpg Lanes 1-3 and 5-8: plasmid PCR of transformed cells that should carry the Promoter-esp-stop764×898 (14,534 bytes) - 21:11, 22 June 2011File:20110621 rsaA.jpg Lanes 1-3 and 5-7: plasmid PCR of colonies that should be carrying ''rsaA'' C-terminal inse694×924 (15,000 bytes) - 21:39, 22 June 2011File:20110623 esp+rsaA 246.jpg ...anes 2-4: ''esp'' from PCR inserted into ''rsaA'' containing pSB1C3; lanes 5-7: ''rsaA'' from PCR inserted into ''esp'' containing pSB1C3; lanes 8-10: '1,012×740 (21,526 bytes) - 23:00, 24 June 2011File:20110623 esp+rsaA 135.jpg ...anes 2-4: ''esp'' from PCR inserted into ''rsaA'' containing pSB1C3; lanes 5-7: ''rsaA'' from PCR inserted into ''esp'' containing pSB1C3; lanes 8-10: '1,010×674 (16,851 bytes) - 23:00, 24 June 2011File:Reference 5.pdf Stephen B. del Cardayre6 & Jay D. Keasling1,2,3,4,5,7(422,198 bytes) - 15:06, 30 June 2011File:20110629 PromoterInserts1.jpg ...pSB1C3 containing ''esp'' and ''rsaA'' C-terminal. Lane 1: ladder; lanes 2-5: PCR with plasmid primers VF2 and VR; lanes 6-9: PCR with promoter specific1,064×962 (69,461 bytes) - 17:43, 30 June 2011File:20110629 PromoterInserts PrsaA.jpg ...pSB1C3 containing ''esp'' and ''rsaA'' C-terminal. Lane 1: ladder; lanes 2-5: PCR with plasmid primers VF2 and VR; lanes 6-9: PCR with promoter specific928×806 (17,728 bytes) - 17:31, 30 June 2011File:20110629 PromoterInserts Both.jpg ...P<html><sub>xyl</sub> insert PCR using plasmid primers VF2 and VR; lanes 4,5: P<sub>rsaA</sub> insert PCR using plasmid primers VF2 and VR; lanes 6,7: P1,066×790 (20,516 bytes) - 17:43, 30 June 2011
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