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  • File:20110628 TestDigest.jpg
    ...SpeI; lane 6: cut with PstI; lane 7: standard uncut DNA fragment for lanes 5 and 6. There is an apparent decrease in size from standard DNA fragments t
    754×810 (18,449 bytes) - 14:29, 29 June 2011
  • File:20110706 MoreColonies.jpg
    Lane 1: ladder; lanes 2-5: colonies that should contain <i>rsaA</i> inserted into pSB1C3 containing < Lanes 5 and 6 show odd results that could be from incomplete digest or successful i
    832×552 (25,851 bytes) - 15:17, 7 July 2011
  • File:20110706 MoreColoniesFollowup.jpg
    Follow up gel of lanes 5 and 6 from the previous gel, redigested for a longer period of time. ...t of <i>rsaA</i> into plasmid already containing <i>esp</i> (previous lane 5); lane 3: digest of miniprep product from colony that should contain an ins
    330×754 (10,054 bytes) - 15:22, 7 July 2011
  • File:20110709dspB+pxyl Colonies5-8.jpg
    ... with Pxyl colonies. Lane 1: ladder; lanes 2-5: WT dspB with Pxyl colonies 5-8; lane 6: standard dspB with no promoter.
    311×385 (5,663 bytes) - 14:03, 5 August 2011
  • File:20110710 optGenes+C3.jpg
    ...timized dspB transformed into pSB1C3 colonies A-E. Lane 1: ladder; lanes 2-5: optimized dspB colonies A-E; lanes 6-9: optimized esp. Colony D shows the
    450×440 (8,008 bytes) - 14:04, 5 August 2011
  • File:20110714 optdspB+rsaA+Pxyl c5-8.jpg
    Figure 10: Colonies 5-8 of Pxyl + dspB + rsaA. Lane1: ladder; lanes 2-5: carry Pxyl + optimized dspB + rsaA; lane 6: standard optimized dspB with r
    322×371 (5,815 bytes) - 14:07, 5 August 2011
  • File:20110718 optespC3 optespPxylC3.jpg
    ...mants that should carry optimized <i>esp</i> behind P<sub>xyl</sub>. Lanes 5 and 7 have the desired gene sequence.
    894×670 (23,076 bytes) - 21:47, 19 July 2011
  • File:20110614 PCR135.jpg
    ...aining ''esp'' ligated with ''rsaA'' that had been digested 20110606; lane 5: PCR product of plasmid containing ''esp''; lanes 6-8: PCR product using VF
    834×1,018 (23,175 bytes) - 19:36, 16 June 2011
  • File:20110617 Pro-esp-stop-rsaA.jpg
    ...: ladder; lanes 2-4 and 6-8: PCR product of transformation survivors; lane 5: control DNA of ''esp'' + ''rsaA''.
    798×930 (20,227 bytes) - 21:03, 26 June 2011
  • File:20110627 esprsaA+promoters 1.jpg
    ...insert into plasmids containing various promoters. Lane 1: ladder; lanes 2-5: inserts into plasmid containing BBa_K081005; lane 6: standard BBa_K081005
    1,062×574 (14,974 bytes) - 15:32, 28 June 2011
  • File:1st Micro Congress.pdf
    In order to meet the 5 Mexican teams and talk about common problems that have arisen along the com
    (1,590,816 bytes) - 22:55, 20 June 2011
  • File:20110620 promotorsTransformationGel.jpg
    ...of two small bands shows contamination of samples. Lane 1: ladder; lanes 2-5: plasmid PCR of transformation survivors.
    408×834 (11,552 bytes) - 20:54, 22 June 2011
  • File:20110621 Promoter-esp-Stop-rsaA-pMR10.jpg
    Lanes 1-3 and 5-8: plasmid PCR of transformed cells that should carry the Promoter-esp-stop
    764×898 (14,534 bytes) - 21:11, 22 June 2011
  • File:20110621 rsaA.jpg
    Lanes 1-3 and 5-7: plasmid PCR of colonies that should be carrying ''rsaA'' C-terminal inse
    694×924 (15,000 bytes) - 21:39, 22 June 2011
  • File:20110623 esp+rsaA 246.jpg
    ...anes 2-4: ''esp'' from PCR inserted into ''rsaA'' containing pSB1C3; lanes 5-7: ''rsaA'' from PCR inserted into ''esp'' containing pSB1C3; lanes 8-10: '
    1,012×740 (21,526 bytes) - 23:00, 24 June 2011
  • File:20110623 esp+rsaA 135.jpg
    ...anes 2-4: ''esp'' from PCR inserted into ''rsaA'' containing pSB1C3; lanes 5-7: ''rsaA'' from PCR inserted into ''esp'' containing pSB1C3; lanes 8-10: '
    1,010×674 (16,851 bytes) - 23:00, 24 June 2011
  • File:Reference 5.pdf
    Stephen B. del Cardayre6 & Jay D. Keasling1,2,3,4,5,7
    (422,198 bytes) - 15:06, 30 June 2011
  • File:20110629 PromoterInserts1.jpg
    ...pSB1C3 containing ''esp'' and ''rsaA'' C-terminal. Lane 1: ladder; lanes 2-5: PCR with plasmid primers VF2 and VR; lanes 6-9: PCR with promoter specific
    1,064×962 (69,461 bytes) - 17:43, 30 June 2011
  • File:20110629 PromoterInserts PrsaA.jpg
    ...pSB1C3 containing ''esp'' and ''rsaA'' C-terminal. Lane 1: ladder; lanes 2-5: PCR with plasmid primers VF2 and VR; lanes 6-9: PCR with promoter specific
    928×806 (17,728 bytes) - 17:31, 30 June 2011
  • File:20110629 PromoterInserts Both.jpg
    ...P<html><sub>xyl</sub> insert PCR using plasmid primers VF2 and VR; lanes 4,5: P<sub>rsaA</sub> insert PCR using plasmid primers VF2 and VR; lanes 6,7: P
    1,066×790 (20,516 bytes) - 17:43, 30 June 2011

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