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Create the page "PSB1C3 vector" on this wiki!

• Team:Waterloo
  ... the Construct: Parts were sequenced into PUC57, digested and ligated into PSB1C3.</div><br /> ... is also isolated for the ligation step. It must also be noted that pSB1C3 vector contains a cut site of SacI, an enzyme that is used in RFC 53. Relocating t
  132 KB (19,352 words) - 19:19, 28 October 2011
• Team:Potsdam Bioware/BioBricks
  To insert mdnED in the vector pSB1C3, the resulting PCR product and the vector were digested with the restriction enzymes EcoRI and SpeI.<br> To insert mdnA in the vector pSB1C3, the resulting PCR product and the vector were digested with the restriction enzymes EcoRI and SpeI.
  59 KB (9,011 words) - 23:32, 28 October 2011
• Team:Columbia-Cooper/GenspaceNotebook
  <p>- NcoI and BamHI digest of pET Vector</p> <p>- Another attempt at NcoI and BamHI digest of pET Vector</p>
  6 KB (1,193 words) - 01:43, 29 September 2011
• Team:Lethbridge/Notebook/Freezer Stocks
  <tr><td>B1 <td>K542006 <td>pSB1C3 <td>Cm <td>Expressing ECFP and EYFP <td>Lethbridge iGEM 2011 <tr><td>B2 <td>K542006 <td>pSB1C3 <td>Cm <td>Expressing ECFP and EYFP <td>Lethbridge iGEM 2011
  7 KB (1,408 words) - 03:15, 29 September 2011
• Team:Hong Kong-CUHK/Notebook
  ...&nbsp;&nbsp;&nbsp; </span></span></b><b>Restriction cut of T7, HR and iGEM vector, ligate them together</b></div> <div>pSB1C3</div>
  1.35 MB (148,320 words) - 17:48, 28 October 2011
• Team:Hong Kong-CUHK/Laboratory/log book
  ...&nbsp;&nbsp;&nbsp; </span></span></b><b>Restriction cut of T7, HR and iGEM vector, ligate them together</b></div> <div>pSB1C3</div>
  1.35 MB (148,301 words) - 15:24, 5 October 2011
• Team:Nevada/Notebook/Weeks58
  ...bp), σ70/pSB1A3 (2190), and rfp (885bp). Incomplete digestion of PDC/ADH/pSB1C3 was apparent for sample #8, so the sample was re-digested before ligation. ...tion vector K654056; GLF; and INV. All genes were contained in the plasmid pSB1C3. Transformants were selected for on chloramphenicol plates and cultured in
  23 KB (3,836 words) - 03:00, 29 September 2011
• Team:Fatih Turkey/test6
  ... vector; because it is declared that all parts must be sent to Registry in pSB1C3. This image shows that this part is confirmed. ...e parts. Moreover, it is wanted that all parts must be sent to Registry in pSB1C3. This part includes a gram positive promoter, RBS sequence and SacB signal
  45 KB (7,068 words) - 09:44, 28 October 2011
• Team:Nevada/Notebook/Weeks14
  ...ceived from iGEM Headquarters. Prepared DNA from iGEM kit for future use (pSB1C3, pSB1A3, pSB1K3 and σ70 constitutive promoter). <br> <br> Transformed iGEM plasmids (pSB1C3, pSB1A3, pSB1K3) and σ70 constitutive promoter (J23101) into NEB10 β stra
  13 KB (2,088 words) - 03:05, 29 September 2011
• Team:Freiburg/Notebook/31 August
  |vector a |vector b
  53 KB (6,142 words) - 01:14, 22 September 2011
• Team:KIT-Kyoto/GFP-MLF実験９月かんたん
  ...ractionkit.aspx">QIAquick Gel Extraction Kit</a>を使用してゲルからpSB1C3のDNAを抽出した。<BR> pSB1C3と開始コドン抜きGFPのライゲーションと形質転換<BR>
  71 KB (8,179 words) - 01:41, 6 October 2011
• Team:WHU-China/calendarjs
  title: 'Transform pDsRed-Express Vector plasmid(1)', title: 'Transform pDsRed-Express Vector plasmid(2)',
  72 KB (13,661 words) - 13:53, 24 October 2011
• Team:Debrecen Hungary/Project
  ...uires two types of plasmids to be cotransfected into a cell; an expression vector, coding a functional NR and a reporter-plasmid, harboring an inducible prom ...ed 3 composite parts and one fully synthetic biology comaptible expression vector from pSB1A3. All of them have been tested by restriction enzyme analysis an
  29 KB (4,562 words) - 22:23, 21 September 2011
• Team:Cornell/Week 13
  ::<u>pSB1C3 Vector Backbone</u> :::40µL pSB1C3 DNA
  8 KB (1,223 words) - 17:23, 18 September 2011
• Team:Michigan/Notebook
  ⇒ Cells were transformed with extra 173 pGLO vector in accordance with the QT protocol. This used stock competent cells found i ⇒ Preliminary designs for a transport vector that can carry surface display components is underway and is currently in t
  73 KB (11,794 words) - 05:23, 29 September 2011
• Team:TzuChiU Formosa/Notebook
  '''Raise E. coli(PSB1C3)''' <pre>PSB1C3 plasmid</pre>
  20 KB (2,430 words) - 17:19, 2 October 2011
• Team:KIT-Kyoto/yokoigawa
  PCR産物とpUAST-flag vectorの制限酵素処理（一回目：<i>Xho</i> I）<br> <tr><td>vector</td></tr>
  32 KB (2,827 words) - 12:42, 2 October 2011
• Team:UNICAMP-EMSE Brazil/Notebook/12 September 2011
  ! scope="col" style="background:#efefef;" | Total size (gene+vector) (bp) ! scope="col" style="background:#efefef;" | Linear vector size (bp)
  6 KB (775 words) - 02:09, 29 September 2011
• Team:Brown-Stanford/Lab/Notebook/Week9
  *Double digested Ana and Nos prom, GFP mut3b, pSB1C3 backbone ...(although buffer was added only for a 50ul rxn); used 15ul in 50ul rxn for pSB1C3
  12 KB (1,954 words) - 18:11, 28 September 2011
• Team:Imperial College London/Project/Auxin/Assembly
  ...ragments (circa 8-10 days) we started to transform our cells with the pVEg+pSB1C3 backbone constructs in order to make enough genetic material for a gibson a ...e a success! We have managed to transform our competent cell colonies with pSB1C3 containing BBa_K398500 and J23100 promoter to produce cell line 6. We also
  12 KB (2,194 words) - 10:17, 14 September 2011