All public logs
From 2011.igem.org
Combined display of all available logs of 2011.igem.org. You can narrow down the view by selecting a log type, the user name (case-sensitive), or the affected page (also case-sensitive).(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)- 03:39, 29 September 2011 Aaring (Talk | contribs) uploaded "File:CaulobacterButton.jpg"
- 03:39, 29 September 2011 Aaring (Talk | contribs) uploaded "File:NotebookButton.jpg"
- 03:37, 29 September 2011 Aaring (Talk | contribs) uploaded "File:BiofilmButton.jpg"
- 03:01, 29 September 2011 Aaring (Talk | contribs) uploaded "File:Esp.png"
- 03:01, 29 September 2011 Aaring (Talk | contribs) uploaded "File:DspB.jpg"
- 01:54, 29 September 2011 Aaring (Talk | contribs) uploaded a new version of "File:PGel2 Labeled.png"
- 01:53, 29 September 2011 Aaring (Talk | contribs) uploaded a new version of "File:PGel1 Labeled.png"
- 01:53, 29 September 2011 Aaring (Talk | contribs) uploaded a new version of "File:GroupPhoto.jpg"
- 01:49, 29 September 2011 Aaring (Talk | contribs) uploaded a new version of "File:GroupPhoto.jpg"
- 23:42, 28 September 2011 Aaring (Talk | contribs) uploaded "File:BiofilmBuster.jpg" (Art courtesy of MB.)
- 04:22, 28 September 2011 Aaring (Talk | contribs) uploaded "File:PGel2 Labeled.png" (Created using ImageJ and Microsoft Office's Powerpoint)
- 04:21, 28 September 2011 Aaring (Talk | contribs) uploaded "File:PGel1 Labeled.png" (Created using ImageJ and Microsoft Office's Powerpoint)
- 04:31, 26 September 2011 Aaring (Talk | contribs) uploaded a new version of "File:LB Profile300.jpg" (Correct proportions.)
- 04:27, 26 September 2011 Aaring (Talk | contribs) uploaded "File:QG Profile300.jpg" (Qimeng. Nothing else need be said.)
- 04:26, 26 September 2011 Aaring (Talk | contribs) uploaded "File:NK Profile300.jpg" (Interesting...)
- 04:25, 26 September 2011 Aaring (Talk | contribs) uploaded "File:LB Profile300.jpg" (Safety first.)
- 04:25, 26 September 2011 Aaring (Talk | contribs) uploaded "File:AA Profile300.jpg" (Huh?)
- 03:14, 26 September 2011 Aaring (Talk | contribs) uploaded "File:GroupPhoto.jpg" (Right to left: Alex Aaring, Dr. Lisa Bowers, Nora Kostow, Qimeng Gao)
- 21:28, 1 August 2011 Aaring (Talk | contribs) uploaded "File:20110801 BBa+oesp+rsaA+pMR10.jpg" (Lane 1: ladder; lanes 2-10: transformants that should contain BBa_K081005, optimized <i>esp</i>, and <i>rsaA</i>. Lanes 2 and 8 show success.)
- 21:26, 1 August 2011 Aaring (Talk | contribs) uploaded "File:20110729 BBa+oesp+rsaA+pMR10 C3.jpg" (Lane 1: ladder; lanes 2,3: PCR of transformants that should carry BBa_K081005, optimized <i>esp</i>, and <i>rsaA</i> in pMR10; lanes 4-10: PCR of transformants that should carry BBa_K081005, optimized <i>esp</i>, and <i>rsaA</i> in pSB1C3. No success.)
- 21:24, 1 August 2011 Aaring (Talk | contribs) uploaded "File:20110728 BD1 Caulobacter.jpg" (Lane 1: ladder; lanes 2-5: PCR of <i>Caulobacter</i> colonies believed to carry BBa_K081005, WT <i>esp</i>, and <i>rsaA</i>. All passed.)
- 21:21, 1 August 2011 Aaring (Talk | contribs) uploaded "File:20110727 BBa+oesp+rsaA.jpg" (Lane 1: ladder; lanes 2-8: digests of miniprep of transformants that should carry BBa_K081005, optimized <i>esp</i>, and <i>rsaA</i>. No success.)
- 21:18, 1 August 2011 Aaring (Talk | contribs) uploaded "File:20110725 Pxyl+oesp+rsaA+pMR10.jpg" (Lane 1: ladder; lanes 2-8: digests of transformants that should contain P<sub>xyl</sub>, optimized <i>esp<i>, and <i>rsaA</i>.)
- 21:09, 1 August 2011 Aaring (Talk | contribs) uploaded "File:20110725 BBa+odspB+rsaA c10-17.jpg" (Lane 1: ladder; lanes 2-9: colony PCR of transformants that should contain BBa_K081005, optimized dspB, and rsaA.)
- 18:41, 25 July 2011 Aaring (Talk | contribs) uploaded "File:Grinnell wiki logo 975px.jpg"
- 16:58, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110723 Pxyl+oesp+rsaA+pMR10.jpg" (Lane 1: ladder; lanes 2-7: PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>esp</i>, and <i>rsaA</i> in pMR10.)
- 16:57, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110723 PrsaA+oesp+rsaA+pMR10.jpg" (Lane 1: ladder; lanes 2-7: PCR of transformants that should carry P<sub>rsaA</sub>, optimized <i>esp</i>, and <i>rsaA</i> in pMR10. Lane 4 was successful.)
- 16:55, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110723 BBa+oesp.jpg" (Gel of digests of miniprepped overnight liquid culture of cells that should carry BBa_K081005 and optimized <i>esp</i>. Lane 1: ladder; lanes 2-10: digests.)
- 16:52, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110722 Bba+oesp.jpg" (Lane 1: ladder; lanes 2-7: transformants that should carry BBa_K081005 and optimized <i>esp</i>.)
- 16:51, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110720 Pxyl+oesp+rsaA oesp+rsaAC3.jpg" (Lane 1: ladder; lanes 2-7: PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>esp</i>, and <i>rsaA</i>; lanes 8-10: transformants that should carry optimized <i>esp</i> with <i>rsaA</i> in pSB1C3.)
- 16:48, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110720 PrsaA+oesp+rsaA oesp+rsaAC3.jpg" (PCR of transformants that should carry P<sub>rsaA</sub>, optimized <i>esp</i>, and <i>rsaA</i> or optimized <i>esp</i> with <i>rsaA</i> in pSB1C3. Lane 1: ladder; lanes 2-7:combo with P<sub>rsaA</sub>; lanes 8-10: combo without promoter. Lanes 3, 8, and 1)
- 16:29, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110722 Pxyl+odspB+rsaA+pMR10.jpg" (PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i> in pMR10. Lane 1: ladder; lanes 2-8: PCR products. )
- 16:27, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110722 BBa+odspB+rsaA+pMR10 c1,5-9.jpg" (PCR of transformants that should carry BBa_K081005, optimized <i>dspB</i>, and <i>rsaA</i> in pMR10. Lane 1: ladder; lanes 2-7: PCR products.)
- 16:25, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110720 Pxyl+odspB+rsaA re-do c7-14 WTdspB c5.jpg" (Gel of PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i>. Lane 1: ladder; lanes 2-9: transformant PCR products; lane 10: digested miniprep of what should have been WT <i>dspB</i>, but showed up as the wrong fra)
- 16:16, 25 July 2011 Aaring (Talk | contribs) uploaded "File:20110719 Pxyl+odspB+rsaA re-do c1,3,4,5,6 std.jpg" (Gel of PCR of transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i>. Lane 1: ladder; lanes 2-6: PCR products; lane 7: standard fragment of <i>dspB</i> with <i>rsaA</i>.)
- 17:27, 24 July 2011 Aaring (Talk | contribs) uploaded "File:Glogo1000.jpg"
- 17:26, 24 July 2011 Aaring (Talk | contribs) uploaded "File:Glogo975.jpg"
- 17:26, 24 July 2011 Aaring (Talk | contribs) uploaded "File:Glogo965.jpg"
- 21:50, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110718 optesprsaAGXIDTSMART optesprsaAC3.jpg" (Lane 1: ladder; lanes 2-7: transformants that should carry optimized <i>esp</i> with <i>rsaA</i> from gel extraction in IDTSMART-AMP. Lanes 3, 4, and 6 have the desired gene sequence.)
- 21:47, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110718 optespC3 optespPxylC3.jpg" (Lane 1: ladder; lanes 2-4: transformants that should carry optimized <i>esp</i> in pSB1C3; lanes 5-10: transformants that should carry optimized <i>esp</i> behind P<sub>xyl</sub>. Lanes 5 and 7 have the desired gene sequence.)
- 21:44, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110718 optespC3 optespPrsaAC3.jpg" (Lane 1: ladder; lanes 2-4: transformants that should carry optimized <i>esp</i> in pSB1C3; lanes 5-10: transformants that should carry optimized <i>esp</i> behind P<sub>rsaA</sub>. Lane 9 has the desired gene sequence.)
- 21:41, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110718 BBa OR PrsaA+optdspB+rsaA pMR10.jpg" (Lane 1: ladder; lanes 2-5: transformants that should carry BBa_K081005, optimized <I>dspB</i>, and <i>rsaA</i> in pMR10; lanes 6-9: transformants that should carry P<sub>rsaA</sub>, optimized <I>dspB</i>, and <i>rsaA</i> in pMR10; lane 10: standard DNA fr)
- 21:09, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110715 Pxyl+optdspB+rsaA c9-14.jpg" (Lane 1: ladder; lanes 2-7: transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i>; lane 8: standard fragment of optimized <i>dspB</i> with <i>rsaA</i>. None of the transformants carried the desired gene sequence.)
- 21:01, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110715 BBa+optdspB+rsaA.jpg" (Lane 1: ladder; lanes 2-5: transformants that should carry BBa_K081005, optimized <i>dspB</i>, and <i>rsaA</i>; lane 6: standard optimized <i>dspB</i> with <i>rsaA</i> fragment. Lanes 3 and 5 carry the desired combination of genes.)
- 20:57, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110714 WTdspB+Pxyl.jpg" (Lane 1: standard optimized <i>dspB</i> with <i>rsaA</i> from PCR; lanes 2,3: transformants that should carry WT <i>dspB</i> behind P<sub>xyl</sub>; lane 4: ladder. No successful transformations.)
- 20:54, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110714 optespIDT.jpg" (Lane 1: ladder; lanes 2-9: transformants that should carry optimized <i>esp</i> in IDTSMART-AMP; lane 10: confirmation of digest of miniprep of strain carrying BBa_K081005, WT <i>esp</i>, and <i>rsaA</i> in pMR10. Lanes 4, 7, and 9 show successful transfo)
- 20:51, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110714 optdspB+rsaA+Pxyl c5-8.jpg" (Lane 1: ladder; lanes 2-5: transformants that should carry P<sub>xyl</sub>, optimized <I>dspB</i>, and <I>rsaA</i>; lane 6: standard optimized <i>dspB</i> with <i>rsaA</i> from PCR. None of these colonies were successful.)
- 20:41, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110714 optdspB+rsaA+PrsaA OR Pxyl.jpg" (Lane 1: ladder; lanes 2-5: transformants that should carry P<sub>rsaA</sub>, optimized <i>dspB</i>, and <i>rsaA</i>; lanes 6-9: transformants that should carry P<sub>xyl</sub>, optimized <i>dspB</i>, and <i>rsaA</i>; lane 10: standard optimized <I>dspB</I)
- 20:36, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110713 optdspBIDT optespIDT.jpg" (<html> Lanes 1-4: transformants that should carry optimized <i>dspB</i> in IDTSMART-AMP; lanes 5,8-10: transformants that should carry optimized <i>esp</i> in IDTSMART-AMP; lane 6: ladder; lane 7: optimized esp standard. None of these transformants ended )
- 20:29, 19 July 2011 Aaring (Talk | contribs) uploaded "File:20110713 optespC3 optespIDTdF.jpg" (<html> Lane 1: ladder; lanes 2-5: colony PCR of transformants that should carry the optimized <i>esp</i> in pSB1C3; lane 6: follow up digest of miniprep of transformant that may carry optimized <i>esp</i> in IDTSMART-AMP. None of these colonies were succe)