http://2011.igem.org/wiki/index.php?title=Special:Contributions/Achala&feed=atom&limit=50&target=Achala&year=&month=2011.igem.org - User contributions [en]2024-03-28T23:04:00ZFrom 2011.igem.orgMediaWiki 1.16.0http://2011.igem.org/World_Championship_Jamboree/Schedule/Practice_SessionsWorld Championship Jamboree/Schedule/Practice Sessions2011-10-27T16:52:27Z<p>Achala: </p>
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<div class="main_item"> <!-- World Jamboree Schedule Goes Here--> <br />
<div class="title">Presentation Practice: Friday November 4th 2011</div><br />
<br />
Use this sign-up sheet to sign up for a slot on Friday night (November 4th) to practice your talk. Note that there will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. <br><br> <br />
<br />
There are a limited number of time slots available on a first-come first-serve basis so please only choose one slot. We cannot match the room that you will ultimately give your presentation in with the practice room. This should, however, give you a chance to practice your talk in a new environment. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time.<br><br><br />
<br />
Also, on Friday, November 4th, there will also be pre-registration available <!-- beginning at <strong>1pm at Compton Lounge</strong> -->. Conference services will be on-site to pass out team registration boxes (see the <a href="https://2011.igem.org/World_Championship_Jamboree/Handbook">Jamboree Handbook</a>). <br><br><br />
<br />
<strong>Note</strong>: Use the wiki edit button to add your team to the schedule (the markup is located at the bottom of the page). Room numbers and locations will be updated as soon as possible. Additional rooms may be added in the coming weeks.<br />
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<table class="calendar" align="center"><h2 class="date"><a name="Friday Practice">Friday, November 4</a></h2><br />
<thead><br />
<tr><br />
<th style="width:100px;">Time</th><br />
<th>RM 56-162 </th><br />
<th>RM 34-101 </th><br />
<th>RM 56-154 </th><br />
<th>RM 32-144 </th><br />
<th>RM 10-250 </th><br />
<th>RM 32-123 </th><br />
<th>RM 56-180 </th><br />
<th>RM 56-114 </th><br />
</tr><br />
</thead><br />
<tbody><br />
<tr class="even"><br />
<th>6:00p - 6:30p</th><br />
<td>Washington</td><br />
<td>Dundee</td><br />
<td>TU München</td><br />
<td>Groningen</td><br />
<td>Lyon-INSA-ENS</td><br />
<td>MIT</td><br />
<td>Fatih Turkey</td><br />
<td>A8</td><br />
</tr><br />
<tr class="odd"><br />
<th>6:30p - 7:00p</th><br />
<td>IIT Madras</td><br />
<td>Art Science Bangalore</td><br />
<td>B3</td><br />
<td>B4</td><br />
<td>B5</td><br />
<td>B5</td><br />
<td>B5</td><br />
<td>B5</td><br />
</tr><br />
<tr class="even"><br />
<th>7:00p - 7:30p</th><br />
<td>NYMU-Taipei</td><br />
<td>Tsinghua-A</td><br />
<td>Paris Bettencourt</td><br />
<td>C4</td><br />
<td>Calgary</td><br />
<td>C6</td><br />
<td>C7</td><br />
<td>C8</td><br />
</tr><br />
<tr class="even"><br />
<th>7:30p - 8:00p</th><br />
<td>Tokyo Tech</td><br />
<td>SYSU-China</td><br />
<td>D3</td><br />
<td>D4</td><br />
<td>D5</td><br />
<td>D6</td><br />
<td>D7</td><br />
<td>D8</td><br />
</tr><br />
<tr class="odd"><br />
<th>8:00p - 8:30p</th><br />
<td>UNICAMP-EMSE</td><br />
<td>USTC-China</td><br />
<td>E3</td><br />
<td>E4</td><br />
<td>Bielefeld-Germany</td><br />
<td>EPF-Lausanne</td><br />
<td>E7</td><br />
<td>E8</td><br />
</tr><br />
<tr class="even"><br />
<th>8:30p - 9:00p</th><br />
<td>ZJU-China</td><br />
<td>USTC-Software</td><br />
<td>Tsinghua</td><br />
<td>F4</td><br />
<td>Osaka</td><br />
<td>F6</td><br />
<td>Peking_R</td><br />
<td>F8</td><br />
</tr><br />
<tr class="odd"><br />
<th>9:00p - 9:30p</th><br />
<td>WHU-China</td><br />
<td>uOttawa</td><br />
<td>G3</td><br />
<td>K.U.Leuven</td><br />
<td>HKUST-Hong_Kong</td><br />
<td>UNITS_Trieste</td><br />
<td>Imperial College London</td><br />
<td>G8</td><br />
</tr><br />
<tr class="even"><br />
<th>9:30p - 10:00p</th><br />
<td>Berkeley</td><br />
<td>Queens_Canada</td><br />
<td>UPO-Sevilla</td><br />
<td>Wisconsin-Madison</td><br />
<td>Tokyo-NoKoGen</td><br />
<td>Harvard</td><br />
<td>Johns Hopkins</td><br />
<td>ETH Zurich</td><br />
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<iframe width="425" height="350" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="http://www.google.com/maps/ms?vpsrc=6&amp;ctz=240&amp;ie=UTF8&amp;msa=0&amp;t=m&amp;msid=208072632249057954752.0004b024a38f4f8325221&amp;source=embed&amp;ll=42.359617,-71.091839&amp;spn=0.000808,0.002135&amp;output=embed"></iframe><br /><small>View <a href="http://www.google.com/maps/ms?vpsrc=6&amp;ctz=240&amp;ie=UTF8&amp;msa=0&amp;t=m&amp;msid=208072632249057954752.0004b024a38f4f8325221&amp;source=embed&amp;ll=42.359617,-71.091839&amp;spn=0.000808,0.002135" style="color:#0000FF;text-align:left">My Saved Places</a> in a larger map</small><br />
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</html></div>Achalahttp://2011.igem.org/File:Speculating.jpgFile:Speculating.jpg2011-10-06T03:34:22Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-06T03:23:16Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
The thing that sets us apart from most IGEM teams is our ultra-tight budget and our desperate attempts at making our own lab equipment.<br />
In Hindi, this is called [http://en.wikipedia.org/wiki/Jugaad "Jugaad"]. Apart from the pleasure of having your own incubator which is pink in color, these exercises help us understand the science better. In these images, we have our microscopes, laminar air flow, a centrifuge and an incubator. We built our own lab by constructing a geodesic <br />
10 feet dome which will house the entire lab and will function as the future ArtScienceBLR headquarters / community BIOlab. <br />
<br />
<br />
[[File:microscope 1.0.jpg|800x533px]]<br />
<br />
[[File:centrifuge.jpg|800x533px]]<br />
<br />
[[File:laminar.jpg|800x533px]]<br />
<br />
[[File:incubator.jpg|800x533px]]<br />
<br />
[[File:dome.jpg|800x533px]]<br />
<br />
<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the watchful eye of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Every BioGeographer needs a toolkit to collect and store their samples.<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:prototype.jpg|350px]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:epindorf slab.jpg|350px]]<br />
<br />
<br />
<br />
'''The Epindorf Bag'''<br />
<br />
[[File:epindorf bag.jpg|350px]]<br />
<br />
<br />
<br />
'''Soil Sampler'''<br />
''..for stylish soil extraction''<br />
<br />
[[File:Soil_Sampler00.jpg]] [[File:Soil_Sampler01.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with (skeptical) Friends'''<br />
<br />
[[File:Balloonmaps01c.png | 750x500px]]<br />
[[File:Balloonmaps02.jpg | 900x500px]]<br />
[[File:SoilSampling01.jpg | 900x500px]]<br />
<br />
<br />
<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
[http://en.wikipedia.org/wiki/David_Cronenberg David Cronenberg's] strange explorations on technology and it's effects on humans are a good place to start for the budding synthetic biologist. <br />
<br />
[[File:Shivers_2.jpg]]<br />
<br />
<br />
''"In the afternoon we watched a movie called Shivers. I cant say it is going to be one of my favorite movies. Though the movie had a pretty interesting story to it, the gory details put me off. One thing i liked a lot about the movie though was that even though it was a 1976 movie, The idea of developing science to such an extent was incredible. The movie shows how a scientist thought of replacing organs in the bodies with parasites. Parasites, which can take over the same function as that organ in the human body. But it didn't work out as he planned and hence the parasites took over the human body and lead it to nasty sexual desires. Aaron and his group apparently had worked on the same concept as the movie for one of their projects last year. They changed the mood patterns in ants and recorded how their entire life style. Same way The scientist in the movie had introduced the parasites into the humans lives. But Madhul raised a very interesting question today. She asked if changing these life style patterns in living things is a good thing or a bad thing. Because in the movie, it lead to mayhem but probably in the ant colony it lead to a good things cause there was no discrimination between the soldiers or the worker ants,or the worker ants and the queen. Which goes back to the question asked on day 4. Whether we have the right to modify lives of living things. This got me thinking a lot. And modifying life can have many different perceptions. I think there should be some laws on how we can modify life patterns in living organisms such that their lives aren't harmed or are caused discomfort"''<br />
<br />
-[http://hackteria.org/wiki/index.php/Achala_Basu '''Achala's diary''']<br />
<br />
We also watched The Fly...and here are some reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
'''Reading [http://www.stanford.edu/dept/HPS/Haraway/CyborgManifesto.html Donna Haraway's cyborg Manifesto](Yawn)'''<br />
<br />
[[File:LisaFoo.gif | CENTER]]<br />
<br />
''Donna Haraway's Cyborg Manifesto coaxes the reader to reconsider stereotypes, assumptions and the true meaning of titular constructs like gender, divinity and organic.The writing style seemed poetic at first but I grew tiresome of her choice of words quickly. Although the manifesto made a few interesting points it was so overladen with unnecessarily esoteric terms that the read was more tedious than interesting. I spent far more time looking up Greek and Latin words than reading the actual article.''<br />
<br />
-'''Bisu's Diary'''<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Some Early Speculative ideas:'''<br />
<br />
<br />
<CENTER>[[File:speculating.jpg|350px]]</CENTER><br />
<br />
<br />
<br />
'''Spicy Bacteria-by Prakrithy'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
<br />
'''Biswajit's Insulin Bacteria'''<br />
<br />
<br />
[[File:Needle-Shy%3F.jpg]]<br />
<br />
''A collection of ideas after a "Soil Mapping Brainstorming session"''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
<br />
<br />
Zoology of the Future<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
<br />
<br />
<br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-06T03:22:06Z<p>Achala: </p>
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'''Building our own BioLab'''<br />
<br />
The thing that sets us apart from most IGEM teams is our ultra-tight budget and our desperate attempts at making our own lab equipment.<br />
In Hindi, this is called [http://en.wikipedia.org/wiki/Jugaad "Jugaad"]. Apart from the pleasure of having your own incubator which is pink in color, these exercises help us understand the science better. In these images, we have our microscopes, laminar air flow, a centrifuge and an incubator. We built our own lab by constructing a geodesic <br />
10 feet dome which will house the entire lab and will function as the future ArtScienceBLR headquarters / community BIOlab. <br />
<br />
<br />
[[File:microscope 1.0.jpg|800x533px]]<br />
<br />
[[File:centrifuge.jpg|800x533px]]<br />
<br />
[[File:laminar.jpg|800x533px]]<br />
<br />
[[File:incubator.jpg|800x533px]]<br />
<br />
[[File:dome.jpg|800x533px]]<br />
<br />
<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the watchful eye of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Every BioGeographer needs a toolkit to collect and store their samples.<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:prototype.jpg|350px]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:epindorf slab.jpg|350px]]<br />
<br />
<br />
<br />
'''The Epindorf Bag'''<br />
<br />
[[File:epindorf bag.jpg|350px]]<br />
<br />
<br />
<br />
'''Soil Sampler'''<br />
''..for stylish soil extraction''<br />
<br />
[[File:Soil_Sampler00.jpg]] [[File:Soil_Sampler01.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with (skeptical) Friends'''<br />
<br />
[[File:Balloonmaps01c.png | 750x500px]]<br />
[[File:Balloonmaps02.jpg | 900x500px]]<br />
[[File:SoilSampling01.jpg | 900x500px]]<br />
<br />
<br />
<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
[http://en.wikipedia.org/wiki/David_Cronenberg David Cronenberg's] strange explorations on technology and it's effects on humans are a good place to start for the budding synthetic biologist. <br />
<br />
[[File:Shivers_2.jpg]]<br />
<br />
<br />
''"In the afternoon we watched a movie called Shivers. I cant say it is going to be one of my favorite movies. Though the movie had a pretty interesting story to it, the gory details put me off. One thing i liked a lot about the movie though was that even though it was a 1976 movie, The idea of developing science to such an extent was incredible. The movie shows how a scientist thought of replacing organs in the bodies with parasites. Parasites, which can take over the same function as that organ in the human body. But it didn't work out as he planned and hence the parasites took over the human body and lead it to nasty sexual desires. Aaron and his group apparently had worked on the same concept as the movie for one of their projects last year. They changed the mood patterns in ants and recorded how their entire life style. Same way The scientist in the movie had introduced the parasites into the humans lives. But Madhul raised a very interesting question today. She asked if changing these life style patterns in living things is a good thing or a bad thing. Because in the movie, it lead to mayhem but probably in the ant colony it lead to a good things cause there was no discrimination between the soldiers or the worker ants,or the worker ants and the queen. Which goes back to the question asked on day 4. Whether we have the right to modify lives of living things. This got me thinking a lot. And modifying life can have many different perceptions. I think there should be some laws on how we can modify life patterns in living organisms such that their lives aren't harmed or are caused discomfort"''<br />
<br />
-[http://hackteria.org/wiki/index.php/Achala_Basu '''Achala's diary''']<br />
<br />
We also watched The Fly...and here are some reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
'''Reading [http://www.stanford.edu/dept/HPS/Haraway/CyborgManifesto.html Donna Haraway's cyborg Manifesto](Yawn)'''<br />
<br />
[[File:LisaFoo.gif | CENTER]]<br />
<br />
''Donna Haraway's Cyborg Manifesto coaxes the reader to reconsider stereotypes, assumptions and the true meaning of titular constructs like gender, divinity and organic.The writing style seemed poetic at first but I grew tiresome of her choice of words quickly. Although the manifesto made a few interesting points it was so overladen with unnecessarily esoteric terms that the read was more tedious than interesting. I spent far more time looking up Greek and Latin words than reading the actual article.''<br />
<br />
-'''Bisu's Diary'''<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Some Early Speculative ideas:'''<br />
<br />
<br />
[[File:ideas3.jpg]]<br />
<br />
<br />
<br />
'''Spicy Bacteria-by Prakrithy'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
<br />
'''Biswajit's Insulin Bacteria'''<br />
<br />
<br />
[[File:Needle-Shy%3F.jpg]]<br />
<br />
''A collection of ideas after a "Soil Mapping Brainstorming session"''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
<br />
<br />
Zoology of the Future<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
<!-- Content ends --><br />
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<!-- Container ends --><br />
</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-06T03:17:26Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
The thing that sets us apart from most IGEM teams is our ultra-tight budget and our desperate attempts at making our own lab equipment.<br />
In Hindi, this is called [http://en.wikipedia.org/wiki/Jugaad "Jugaad"]. Apart from the pleasure of having your own incubator which is pink in color, these exercises help us understand the science better. In these images, we have our microscopes, laminar air flow, a centrifuge and an incubator. We built our own lab by constructing a geodesic <br />
10 feet dome which will house the entire lab and will function as the future ArtScienceBLR headquarters / community BIOlab. <br />
<br />
<br />
[[File:microscope 1.0.jpg|800x533px]]<br />
<br />
[[File:centrifuge.jpg|800x533px]]<br />
<br />
[[File:laminar.jpg|800x533px]]<br />
<br />
[[File:incubator.jpg|800x533px]]<br />
<br />
[[File:dome.jpg|800x533px]]<br />
<br />
<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the watchful eye of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Every BioGeographer needs a toolkit to collect and store their samples.<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:prototype.jpg|350px]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:epindorf slab.jpg|350px]]<br />
<br />
<br />
<br />
'''The Epindorf Bag'''<br />
<br />
[[File:epindorf bag.jpg|350px]]<br />
<br />
<br />
<br />
'''Soil Sampler'''<br />
''..for stylish soil extraction''<br />
<br />
[[File:Soil_Sampler00.jpg]] [[File:Soil_Sampler01.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with (skeptical) Friends'''<br />
<br />
[[File:Balloonmaps01c.png | 750x500px]]<br />
[[File:Balloonmaps02.jpg | 900x500px]]<br />
[[File:SoilSampling01.jpg | 900x500px]]<br />
<br />
<br />
<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
[http://en.wikipedia.org/wiki/David_Cronenberg David Cronenberg's] strange explorations on technology and it's effects on humans are a good place to start for the budding synthetic biologist. <br />
<br />
[[File:Shivers_2.jpg]]<br />
<br />
<br />
''"In the afternoon we watched a movie called Shivers. I cant say it is going to be one of my favorite movies. Though the movie had a pretty interesting story to it, the gory details put me off. One thing i liked a lot about the movie though was that even though it was a 1976 movie, The idea of developing science to such an extent was incredible. The movie shows how a scientist thought of replacing organs in the bodies with parasites. Parasites, which can take over the same function as that organ in the human body. But it didn't work out as he planned and hence the parasites took over the human body and lead it to nasty sexual desires. Aaron and his group apparently had worked on the same concept as the movie for one of their projects last year. They changed the mood patterns in ants and recorded how their entire life style. Same way The scientist in the movie had introduced the parasites into the humans lives. But Madhul raised a very interesting question today. She asked if changing these life style patterns in living things is a good thing or a bad thing. Because in the movie, it lead to mayhem but probably in the ant colony it lead to a good things cause there was no discrimination between the soldiers or the worker ants,or the worker ants and the queen. Which goes back to the question asked on day 4. Whether we have the right to modify lives of living things. This got me thinking a lot. And modifying life can have many different perceptions. I think there should be some laws on how we can modify life patterns in living organisms such that their lives aren't harmed or are caused discomfort"''<br />
<br />
-[http://hackteria.org/wiki/index.php/Achala_Basu '''Achala's diary''']<br />
<br />
We also watched The Fly...and here are some reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
'''Reading [http://www.stanford.edu/dept/HPS/Haraway/CyborgManifesto.html Donna Haraway's cyborg Manifesto](Yawn)'''<br />
<br />
[[File:LisaFoo.gif | CENTER]]<br />
<br />
''Donna Haraway's Cyborg Manifesto coaxes the reader to reconsider stereotypes, assumptions and the true meaning of titular constructs like gender, divinity and organic.The writing style seemed poetic at first but I grew tiresome of her choice of words quickly. Although the manifesto made a few interesting points it was so overladen with unnecessarily esoteric terms that the read was more tedious than interesting. I spent far more time looking up Greek and Latin words than reading the actual article.''<br />
<br />
-'''Bisu's Diary'''<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Some Early Speculative ideas:'''<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Spicy Bacteria-by Prakrithy'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
<br />
'''Biswajit's Insulin Bacteria'''<br />
<br />
<br />
[[File:Needle-Shy%3F.jpg]]<br />
<br />
''A collection of ideas after a "Soil Mapping Brainstorming session"''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
<br />
<br />
Zoology of the Future<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
<!-- Content ends --><br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-06T03:15:36Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
The thing that sets us apart from most IGEM teams is our ultra-tight budget and our desperate attempts at making our own lab equipment.<br />
In Hindi, this is called [http://en.wikipedia.org/wiki/Jugaad "Jugaad"]. Apart from the pleasure of having your own incubator which is pink in color, these exercises help us understand the science better. In these images, we have our microscopes, laminar air flow, a centrifuge and an incubator. We built our own lab by constructing a geodesic <br />
10 feet dome which will house the entire lab and will function as the future ArtScienceBLR headquarters / community BIOlab. <br />
<br />
<br />
[[File:microscope 1.0.jpg|800x533px]]<br />
<br />
[[File:centrifuge.jpg|800x533px]]<br />
<br />
[[File:laminar.jpg|800x533px]]<br />
<br />
[[File:incubator.jpg|800x533px]]<br />
<br />
[[File:dome.jpg|800x533px]]<br />
<br />
<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the watchful eye of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Every BioGeographer needs a toolkit to collect and store their samples.<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:prototype.jpg|350px]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:epindorf slab.jpg|350px]]<br />
<br />
<br />
<br />
'''The Epindorf Bag'''<br />
<br />
[[File:epindorf bag.jpg|350px]]<br />
<br />
<br />
<br />
'''Soil Sampler'''<br />
''..for stylish soil extraction''<br />
<br />
[[File:Soil_Sampler00.jpg]] [[File:Soil_Sampler01.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with (skeptical) Friends'''<br />
<br />
[[File:Balloonmaps01c.png | 750x500px]]<br />
[[File:Balloonmaps02.jpg | 900x500px]]<br />
[[File:SoilSampling01.jpg | 900x500px]]<br />
<br />
<br />
<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
[http://en.wikipedia.org/wiki/David_Cronenberg David Cronenberg's] strange explorations on technology and it's effects on humans are a good place to start for the budding synthetic biologist. <br />
<br />
[[File:Shivers_2.jpg]]<br />
<br />
<br />
''"In the afternoon we watched a movie called Shivers. I cant say it is going to be one of my favorite movies. Though the movie had a pretty interesting story to it, the gory details put me off. One thing i liked a lot about the movie though was that even though it was a 1976 movie, The idea of developing science to such an extent was incredible. The movie shows how a scientist thought of replacing organs in the bodies with parasites. Parasites, which can take over the same function as that organ in the human body. But it didn't work out as he planned and hence the parasites took over the human body and lead it to nasty sexual desires. Aaron and his group apparently had worked on the same concept as the movie for one of their projects last year. They changed the mood patterns in ants and recorded how their entire life style. Same way The scientist in the movie had introduced the parasites into the humans lives. But Madhul raised a very interesting question today. She asked if changing these life style patterns in living things is a good thing or a bad thing. Because in the movie, it lead to mayhem but probably in the ant colony it lead to a good things cause there was no discrimination between the soldiers or the worker ants,or the worker ants and the queen. Which goes back to the question asked on day 4. Whether we have the right to modify lives of living things. This got me thinking a lot. And modifying life can have many different perceptions. I think there should be some laws on how we can modify life patterns in living organisms such that their lives aren't harmed or are caused discomfort"''<br />
<br />
-[http://hackteria.org/wiki/index.php/Achala_Basu '''Achala's diary''']<br />
<br />
We also watched The Fly...and here are some reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
'''Reading [http://www.stanford.edu/dept/HPS/Haraway/CyborgManifesto.html Donna Haraway's cyborg Manifesto](Yawn)'''<br />
<br />
[[File:LisaFoo.gif | CENTER]]<br />
<br />
''Donna Haraway's Cyborg Manifesto coaxes the reader to reconsider stereotypes, assumptions and the true meaning of titular constructs like gender, divinity and organic.The writing style seemed poetic at first but I grew tiresome of her choice of words quickly. Although the manifesto made a few interesting points it was so overladen with unnecessarily esoteric terms that the read was more tedious than interesting. I spent far more time looking up Greek and Latin words than reading the actual article.''<br />
<br />
-'''Bisu's Diary'''<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Spicy Bacteria-by Prakrithy'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
<br />
'''Biswajit's Insulin Bacteria'''<br />
<br />
<br />
[[File:Needle-Shy%3F.jpg]]<br />
<br />
''A collection of ideas after a "Soil Mapping Brainstorming session"''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
<br />
<br />
Zoology of the Future<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-06T03:12:31Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
The thing that sets us apart from most IGEM teams is our ultra-tight budget and our desperate attempts at making our own lab equipment.<br />
In Hindi, this is called [http://en.wikipedia.org/wiki/Jugaad "Jugaad"]. Apart from the pleasure of having your own incubator which is pink in color, these exercises help us understand the science better. In these images, we have our microscopes, laminar air flow, a centrifuge and an incubator. We built our own lab by constructing a geodesic <br />
10 feet dome which will house the entire lab and will function as the future ArtScienceBLR headquarters / community BIOlab. <br />
<br />
<br />
[[File:microscope 1.0.jpg|800x533px]]<br />
<br />
[[File:centrifuge.jpg|800x533px]]<br />
<br />
[[File:laminar.jpg|800x533px]]<br />
<br />
[[File:incubator.jpg|800x533px]]<br />
<br />
[[File:dome.jpg|800x533px]]<br />
<br />
<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the watchful eye of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Every BioGeographer needs a toolkit to collect and store their samples.<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:prototype.jpg|350px]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:epindorf slab.jpg|350px]]<br />
<br />
<br />
<br />
'''The Epindorf Bag'''<br />
<br />
[[File:epindorf bag.jpg|350px]]<br />
<br />
<br />
<br />
'''Soil Sampler'''<br />
''..for stylish soil extraction''<br />
<br />
[[File:Soil_Sampler00.jpg]] [[File:Soil_Sampler01.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with (skeptical) Friends'''<br />
<br />
[[File:Balloonmaps01c.png | 750x500px]]<br />
[[File:Balloonmaps02.jpg | 900x500px]]<br />
[[File:SoilSampling01.jpg | 900x500px]]<br />
<br />
<br />
<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
[http://en.wikipedia.org/wiki/David_Cronenberg David Cronenberg's] strange explorations on technology and it's effects on humans are a good place to start for the budding synthetic biologist. <br />
<br />
[[File:Shivers_2.jpg]]<br />
<br />
<br />
''"In the afternoon we watched a movie called Shivers. I cant say it is going to be one of my favorite movies. Though the movie had a pretty interesting story to it, the gory details put me off. One thing i liked a lot about the movie though was that even though it was a 1976 movie, The idea of developing science to such an extent was incredible. The movie shows how a scientist thought of replacing organs in the bodies with parasites. Parasites, which can take over the same function as that organ in the human body. But it didn't work out as he planned and hence the parasites took over the human body and lead it to nasty sexual desires. Aaron and his group apparently had worked on the same concept as the movie for one of their projects last year. They changed the mood patterns in ants and recorded how their entire life style. Same way The scientist in the movie had introduced the parasites into the humans lives. But Madhul raised a very interesting question today. She asked if changing these life style patterns in living things is a good thing or a bad thing. Because in the movie, it lead to mayhem but probably in the ant colony it lead to a good things cause there was no discrimination between the soldiers or the worker ants,or the worker ants and the queen. Which goes back to the question asked on day 4. Whether we have the right to modify lives of living things. This got me thinking a lot. And modifying life can have many different perceptions. I think there should be some laws on how we can modify life patterns in living organisms such that their lives aren't harmed or are caused discomfort"''<br />
<br />
-[http://hackteria.org/wiki/index.php/Achala_Basu '''Achala's diary''']<br />
<br />
We also watched The Fly...and here are some reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
'''Reading [http://www.stanford.edu/dept/HPS/Haraway/CyborgManifesto.html Donna Haraway's cyborg Manifesto](Yawn)'''<br />
<br />
[[File:LisaFoo.gif | CENTER]]<br />
<br />
''Donna Haraway's Cyborg Manifesto coaxes the reader to reconsider stereotypes, assumptions and the true meaning of titular constructs like gender, divinity and organic.The writing style seemed poetic at first but I grew tiresome of her choice of words quickly. Although the manifesto made a few interesting points it was so overladen with unnecessarily esoteric terms that the read was more tedious than interesting. I spent far more time looking up Greek and Latin words than reading the actual article.''<br />
<br />
-'''Bisu's Diary'''<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:ideas.jpg|300px]][[File:ideas2.jpg|300px]][[File:ideas3.jpg|300px]]<br />
<br />
<br />
<br />
<br />
<br />
'''Spicy Bacteria-by Prakrithy'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
<br />
'''Biswajit's Insulin Bacteria'''<br />
<br />
<br />
[[File:Needle-Shy%3F.jpg]]<br />
<br />
''A collection of ideas after a "Soil Mapping Brainstorming session"''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
<br />
<br />
Zoology of the Future<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
<!-- Content ends --><br />
</div><br />
<!-- Container ends --><br />
</html></div>Achalahttp://2011.igem.org/File:Ideas3.jpgFile:Ideas3.jpg2011-10-06T03:09:58Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Ideas2.jpgFile:Ideas2.jpg2011-10-06T03:09:26Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Ideas.jpgFile:Ideas.jpg2011-10-06T03:06:18Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-06T02:54:16Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
The thing that sets us apart from most IGEM teams is our ultra-tight budget and our desperate attempts at making our own lab equipment.<br />
In Hindi, this is called [http://en.wikipedia.org/wiki/Jugaad "Jugaad"]. Apart from the pleasure of having your own incubator which is pink in color, these exercises help us understand the science better. In these images, we have our microscopes, laminar air flow, a centrifuge and an incubator. We built our own lab by constructing a geodesic <br />
10 feet dome which will house the entire lab and will function as the future ArtScienceBLR headquarters / community BIOlab. <br />
<br />
<br />
[[File:microscope 1.0.jpg|800x533px]]<br />
<br />
[[File:centrifuge.jpg|800x533px]]<br />
<br />
[[File:laminar.jpg|800x533px]]<br />
<br />
[[File:incubator.jpg|800x533px]]<br />
<br />
[[File:dome.jpg|800x533px]]<br />
<br />
<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the watchful eye of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Every BioGeographer needs a toolkit to collect and store their samples.<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:prototype.jpg|350px]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:epindorf slab.jpg|350px]]<br />
<br />
<br />
<br />
'''The Epindorf Bag'''<br />
<br />
[[File:epindorf bag.jpg|350px]]<br />
<br />
<br />
<br />
'''Soil Sampler'''<br />
''..for stylish soil extraction''<br />
<br />
[[File:Soil_Sampler00.jpg]] [[File:Soil_Sampler01.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with (skeptical) Friends'''<br />
<br />
[[File:Balloonmaps01c.png | 750x500px]]<br />
[[File:Balloonmaps02.jpg | 900x500px]]<br />
[[File:SoilSampling01.jpg | 900x500px]]<br />
<br />
<br />
<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
[http://en.wikipedia.org/wiki/David_Cronenberg David Cronenberg's] strange explorations on technology and it's effects on humans are a good place to start for the budding synthetic biologist. <br />
<br />
[[File:Shivers_2.jpg]]<br />
<br />
<br />
''"In the afternoon we watched a movie called Shivers. I cant say it is going to be one of my favorite movies. Though the movie had a pretty interesting story to it, the gory details put me off. One thing i liked a lot about the movie though was that even though it was a 1976 movie, The idea of developing science to such an extent was incredible. The movie shows how a scientist thought of replacing organs in the bodies with parasites. Parasites, which can take over the same function as that organ in the human body. But it didn't work out as he planned and hence the parasites took over the human body and lead it to nasty sexual desires. Aaron and his group apparently had worked on the same concept as the movie for one of their projects last year. They changed the mood patterns in ants and recorded how their entire life style. Same way The scientist in the movie had introduced the parasites into the humans lives. But Madhul raised a very interesting question today. She asked if changing these life style patterns in living things is a good thing or a bad thing. Because in the movie, it lead to mayhem but probably in the ant colony it lead to a good things cause there was no discrimination between the soldiers or the worker ants,or the worker ants and the queen. Which goes back to the question asked on day 4. Whether we have the right to modify lives of living things. This got me thinking a lot. And modifying life can have many different perceptions. I think there should be some laws on how we can modify life patterns in living organisms such that their lives aren't harmed or are caused discomfort"''<br />
<br />
-[http://hackteria.org/wiki/index.php/Achala_Basu '''Achala's diary''']<br />
<br />
We also watched The Fly...and here are some reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
'''Reading [http://www.stanford.edu/dept/HPS/Haraway/CyborgManifesto.html Donna Haraway's cyborg Manifesto](Yawn)'''<br />
<br />
[[File:LisaFoo.gif | CENTER]]<br />
<br />
''Donna Haraway's Cyborg Manifesto coaxes the reader to reconsider stereotypes, assumptions and the true meaning of titular constructs like gender, divinity and organic.The writing style seemed poetic at first but I grew tiresome of her choice of words quickly. Although the manifesto made a few interesting points it was so overladen with unnecessarily esoteric terms that the read was more tedious than interesting. I spent far more time looking up Greek and Latin words than reading the actual article.''<br />
<br />
-'''Bisu's Diary'''<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:ideas.jpg|350px]]<br />
[[File:ideas2.jpg|350px]]<br />
[[File:ideas3.jpg|350px]]<br />
<br />
<br />
<br />
<br />
<br />
<br />
'''Spicy Bacteria-by Prakrithy'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
[[File:Needle-Shy%3F.jpg]]<br />
<br />
''A collection of ideas after a "Soil Mapping Brainstorming session"''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
<br />
<br />
Zoology of the Future<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
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</html></div>Achalahttp://2011.igem.org/File:Microscope_1.0.jpgFile:Microscope 1.0.jpg2011-10-05T21:39:15Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Epindorf_bag.jpgFile:Epindorf bag.jpg2011-10-05T21:33:21Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Epindorf_slab.jpgFile:Epindorf slab.jpg2011-10-05T21:31:33Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Cloth_toolkit.jpgFile:Cloth toolkit.jpg2011-10-05T21:31:14Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Prototype.jpgFile:Prototype.jpg2011-10-05T21:30:28Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Dome.jpgFile:Dome.jpg2011-10-05T21:26:43Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Incubator.jpgFile:Incubator.jpg2011-10-05T21:21:17Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Laminar.jpgFile:Laminar.jpg2011-10-05T21:20:20Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Centrifuge.jpgFile:Centrifuge.jpg2011-10-05T21:19:37Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-05T21:18:00Z<p>Achala: </p>
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'''Building our own BioLab'''<br />
<br />
One of our main aspects of doing igem everyear is making our own biolab from scrap, i.e jugaad.<br />
Using alternatives and different methods to carry out a completely different purpose. <br />
We started off with building a microscope from a webcam, and soon we were making a centrifuge out of a hand blender. We also made a laminar air flow, a desicator and an incubator from different materials.<br />
We built our own lab by constructing a geodesic 10 feet metal dome which we covered with tarp.<br />
<br />
Featured in this image is our own DIY microscope, sterile hood, incubator and a geodesic Biodome which houses our entire lab.<br />
<br />
<br />
[[File:microscope 1.0.jpg|350px]]<br />
<br />
<br />
[[File:centrifuge.jpg|350px]]<br />
<br />
<br />
[[File:laminar.jpg|350px]]<br />
<br />
<br />
[[File:incubator.jpg|350px]]<br />
<br />
<br />
[[File:dome.jpg|350px]]<br />
<br />
<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the guidance of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Before our expeditions to collect soil samples, we came up with a tool kit that holds all the essentials required to be carried on the field while working:<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:prototype.jpg|350px]]<br />
<br />
<br />
<br />
<br />
'''Fold-able cloth toolkit'''<br />
<br />
[[File:cloth toolkit.jpg|350px]]<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:epindorf slab.jpg|350px]]<br />
<br />
<br />
<br />
'''Epindorf Bag'''<br />
<br />
[[File:epindorf bag.jpg|350px]]<br />
<br />
<br />
<br />
'''Soil Sampler'''<br />
<br />
[[File:Soil_Sampler.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with Friends'''<br />
<br />
As we all know aerial photography is quite expensive; owing to our tight budget, we had to come up with a simple and cheaper way of the same- balloon mapping. <br />
<br />
We invited students from our campus to show them our cost effective way of aerial photography. They were quite impressed of how a easy it was to make a useful gadget out of simple day to day objects- helium filled balloons, a strong thread and a digital camera.<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
Shivers (a movie directed by David Cronenberg) is about a doctor’s attempts to bring out the inner sexual animal in man, using a parasite. He fooled his co-workers, and sponsors, into thinking that he was working on creating an alternative to organ transplant, by using parasites. The doctor was unable to accurately predict what the modified parasite would do. This lead to the parasite spreading from his teenaged mistress to quite a few other residents of a “sterile” high-end apartment block, and spreading uncontrollable sexual desire.<br />
This movie highlighted the unpredictable nature of the effects, the consequences of any modifications that we make in any organisms. It also highlights the unpredictability of the behaviour of the organisms. The behaviour of the modified organism in the laboratory is not adequate enough to predict its behaviour in the outside (real) world. The parasites modified an entire society and, in a manner, the entire purpose of a community.<br />
<br />
We also watched The Fly...and here are a few of the reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
Spices like chillies & pepper feel "hot" beacuse they contain certain chemicals like capsaicin and piperine. Our body (mainly our mouth) feels a sensation of burning pain, as a result of these chemicals. Our sensory neurons contain a functional cDNA encoding for a capsaicin receptor. The receptor is a non-selective cation channel that is also activated by increases in temperature. Similarly, other spices will all have acting chemicals, mostly organic. Each spice will also have corresponding receptors, or mechanisms, to induce responses. Most of the spices and condiments used are plant parts. This implies that they do contain cells (living or dead. Cells contain DNA, which would be responsible for the production of the chemicals responsible for the flavours of the spices. We would be able to identify the exact DNA sequences that code for the necessary chemicals by DNA sequencing. Once the sequences are identified, they can be introduced into the DNA of a suitable, safe bacteria. This bacteria would produce the different chemicals (for the spices) in certain fixed quantities when introduced into the food. This would reduce the chances of dishes getting "spoilt" due to the usage of incorrect mixtures and quantities of spices.<br />
<br />
<br />
''Brainstorm of Soil Mapping''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
"Space Dusters" / Space Crashers = Mediorite Landing<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
Tracing the path of Ganga<br />
<br />
Zoology of the Future<br />
Junkyard is separated out by object - sample each site<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
Defense Areas / BSF DRDO Air Force<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
Deadly Differences<br />
Samples from different types'/societies'/religions' burial grounds/crematoriums<br />
<br />
Soil "Classes"<br />
Soils from different classes of places<br />
"Holy", Royal, "Dirty", Common, etc<br />
<br />
Life Soil<br />
Trace all locations in a persons life<br />
Birth, Education, Settlement, Entertainment, Employment, Retirement, Health, Death, Graveyard, Paranormal activity<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-05T21:17:03Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
One of our main aspects of doing igem everyear is making our own biolab from scrap, i.e jugaad.<br />
Using alternatives and different methods to carry out a completely different purpose. <br />
We started off with building a microscope from a webcam, and soon we were making a centrifuge out of a hand blender. We also made a laminar air flow, a desicator and an incubator from different materials.<br />
We built our own lab by constructing a geodesic 10 feet metal dome which we covered with tarp.<br />
<br />
Featured in this image is our own DIY microscope, sterile hood, incubator and a geodesic Biodome which houses our entire lab.<br />
<br />
<br />
[[File:microscope.jpg|350px]]<br />
<br />
<br />
[[File:centrifuge.jpg|350px]]<br />
<br />
<br />
[[File:laminar.jpg|350px]]<br />
<br />
<br />
[[File:incubator.jpg|350px]]<br />
<br />
<br />
[[File:dome.jpg|350px]]<br />
<br />
<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the guidance of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Before our expeditions to collect soil samples, we came up with a tool kit that holds all the essentials required to be carried on the field while working:<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:prototype.jpg|350px]]<br />
<br />
<br />
<br />
<br />
'''Fold-able cloth toolkit'''<br />
<br />
[[File:cloth toolkit.jpg|350px]]<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:epindorf slab.jpg|350px]]<br />
<br />
<br />
<br />
'''Epindorf Bag'''<br />
<br />
[[File:epindorf bag.jpg|350px]]<br />
<br />
<br />
<br />
'''Soil Sampler'''<br />
<br />
[[File:Soil_Sampler.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with Friends'''<br />
<br />
As we all know aerial photography is quite expensive; owing to our tight budget, we had to come up with a simple and cheaper way of the same- balloon mapping. <br />
<br />
We invited students from our campus to show them our cost effective way of aerial photography. They were quite impressed of how a easy it was to make a useful gadget out of simple day to day objects- helium filled balloons, a strong thread and a digital camera.<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
Shivers (a movie directed by David Cronenberg) is about a doctor’s attempts to bring out the inner sexual animal in man, using a parasite. He fooled his co-workers, and sponsors, into thinking that he was working on creating an alternative to organ transplant, by using parasites. The doctor was unable to accurately predict what the modified parasite would do. This lead to the parasite spreading from his teenaged mistress to quite a few other residents of a “sterile” high-end apartment block, and spreading uncontrollable sexual desire.<br />
This movie highlighted the unpredictable nature of the effects, the consequences of any modifications that we make in any organisms. It also highlights the unpredictability of the behaviour of the organisms. The behaviour of the modified organism in the laboratory is not adequate enough to predict its behaviour in the outside (real) world. The parasites modified an entire society and, in a manner, the entire purpose of a community.<br />
<br />
We also watched The Fly...and here are a few of the reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
Spices like chillies & pepper feel "hot" beacuse they contain certain chemicals like capsaicin and piperine. Our body (mainly our mouth) feels a sensation of burning pain, as a result of these chemicals. Our sensory neurons contain a functional cDNA encoding for a capsaicin receptor. The receptor is a non-selective cation channel that is also activated by increases in temperature. Similarly, other spices will all have acting chemicals, mostly organic. Each spice will also have corresponding receptors, or mechanisms, to induce responses. Most of the spices and condiments used are plant parts. This implies that they do contain cells (living or dead. Cells contain DNA, which would be responsible for the production of the chemicals responsible for the flavours of the spices. We would be able to identify the exact DNA sequences that code for the necessary chemicals by DNA sequencing. Once the sequences are identified, they can be introduced into the DNA of a suitable, safe bacteria. This bacteria would produce the different chemicals (for the spices) in certain fixed quantities when introduced into the food. This would reduce the chances of dishes getting "spoilt" due to the usage of incorrect mixtures and quantities of spices.<br />
<br />
<br />
''Brainstorm of Soil Mapping''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
"Space Dusters" / Space Crashers = Mediorite Landing<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
Tracing the path of Ganga<br />
<br />
Zoology of the Future<br />
Junkyard is separated out by object - sample each site<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
Defense Areas / BSF DRDO Air Force<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
Deadly Differences<br />
Samples from different types'/societies'/religions' burial grounds/crematoriums<br />
<br />
Soil "Classes"<br />
Soils from different classes of places<br />
"Holy", Royal, "Dirty", Common, etc<br />
<br />
Life Soil<br />
Trace all locations in a persons life<br />
Birth, Education, Settlement, Entertainment, Employment, Retirement, Health, Death, Graveyard, Paranormal activity<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
<!-- Content ends --><br />
</div><br />
<!-- Container ends --><br />
</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2011-10-05T21:04:06Z<p>Achala: </p>
<hr />
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'''Outreach'''<br />
<br />
In its inherent exploration, this project has engaged with various communities. From artists to school children, scientists to organic farmers,this project has interacted with multiple ideas and observations of relevance to the notion of citizen science and the future of our environments. <br />
<br />
'''Walking through the rain forest with Sujata Goel, an organic farmer with a Phd in Botany'''<br />
<br />
<br />
[[File:aa.jpg]]<br />
<br />
[[File:bb.jpg]]<br />
<br />
[[File:cc.jpg]]<br />
<br />
[[File:dd.jpg]]<br />
<br />
<br />
'''Balloon Mapping and Soil Sampling with School Children and our friends'''<br />
<br />
'''A handBook for Children(coming and get this from us at H.K)'''<br />
<br />
<br />
<br />
<br />
<br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/OutreachTeam:ArtScienceBangalore/Outreach2011-10-05T21:03:19Z<p>Achala: </p>
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'''Outreach'''<br />
<br />
In its inherent exploration, this project has engaged with various communities. From artists to school children, scientists to organic farmers,this project has interacted with multiple ideas and observations of relevance to the notion of citizen science and the future of our environments. <br />
<br />
'''Walking through the rain forest with Sujata Goel, an organic farmer with a Phd in Botany'''<br />
<br />
<br />
[[File:a.jpg]]<br />
<br />
[[File:b.jpg]]<br />
<br />
[[File:c.jpg]]<br />
<br />
[[File:d.jpg]]<br />
<br />
<br />
'''Balloon Mapping and Soil Sampling with School Children and our friends'''<br />
<br />
'''A handBook for Children(coming and get this from us at H.K)'''<br />
<br />
<br />
<br />
<br />
<br />
<html><br />
</div><br />
<!-- Content ends --><br />
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<!-- Container ends --><br />
</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-05T21:00:30Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
One of our main aspects of doing igem everyear is making our own biolab from scrap, i.e jugaad.<br />
Using alternatives and different methods to carry out a completely different purpose. <br />
We started off with building a microscope from a webcam, and soon we were making a centrifuge out of a hand blender. We also made a laminar air flow, a desicator and an incubator from different materials.<br />
We built our own lab by constructing a geodesic 10 feet metal dome which we covered with tarp.<br />
<br />
Featured in this image is our own DIY microscope, sterile hood, incubator and a geodesic Biodome which houses our entire lab.<br />
<br />
<br />
[[File:collage1.jpg|350px]]<br />
<br />
<br />
[[File:collage2.jpg|350px]]<br />
<br />
<br />
[[File:collage3.jpg|350px]]<br />
<br />
<br />
[[File:collage4.jpg|350px]]<br />
<br />
<br />
[[File:collage5.jpg|350px]]<br />
<br />
<br />
[[File:collage7.jpg]]<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the guidance of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Before our expeditions to collect soil samples, we came up with a tool kit that holds all the essentials required to be carried on the field while working:<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:collage8.jpg|350px]]<br />
<br />
<br />
<br />
<br />
'''Fold-able cloth toolkit'''<br />
<br />
[[File:collage9.jpg|350px]]<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:collage10.jpg|350px]]<br />
<br />
<br />
<br />
'''Epindorf Bag'''<br />
<br />
[[File:collage11.jpg|350px]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with Friends'''<br />
<br />
As we all know aerial photography is quite expensive; owing to our tight budget, we had to come up with a simple and cheaper way of the same- balloon mapping. <br />
<br />
We invited students from our campus to show them our cost effective way of aerial photography. They were quite impressed of how a easy it was to make a useful gadget out of simple day to day objects- helium filled balloons, a strong thread and a digital camera.<br />
<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
Shivers (a movie directed by David Cronenberg) is about a doctor’s attempts to bring out the inner sexual animal in man, using a parasite. He fooled his co-workers, and sponsors, into thinking that he was working on creating an alternative to organ transplant, by using parasites. The doctor was unable to accurately predict what the modified parasite would do. This lead to the parasite spreading from his teenaged mistress to quite a few other residents of a “sterile” high-end apartment block, and spreading uncontrollable sexual desire.<br />
This movie highlighted the unpredictable nature of the effects, the consequences of any modifications that we make in any organisms. It also highlights the unpredictability of the behaviour of the organisms. The behaviour of the modified organism in the laboratory is not adequate enough to predict its behaviour in the outside (real) world. The parasites modified an entire society and, in a manner, the entire purpose of a community.<br />
<br />
We also watched The Fly...and here are a few of the reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
Spices like chillies & pepper feel "hot" beacuse they contain certain chemicals like capsaicin and piperine. Our body (mainly our mouth) feels a sensation of burning pain, as a result of these chemicals. Our sensory neurons contain a functional cDNA encoding for a capsaicin receptor. The receptor is a non-selective cation channel that is also activated by increases in temperature. Similarly, other spices will all have acting chemicals, mostly organic. Each spice will also have corresponding receptors, or mechanisms, to induce responses. Most of the spices and condiments used are plant parts. This implies that they do contain cells (living or dead. Cells contain DNA, which would be responsible for the production of the chemicals responsible for the flavours of the spices. We would be able to identify the exact DNA sequences that code for the necessary chemicals by DNA sequencing. Once the sequences are identified, they can be introduced into the DNA of a suitable, safe bacteria. This bacteria would produce the different chemicals (for the spices) in certain fixed quantities when introduced into the food. This would reduce the chances of dishes getting "spoilt" due to the usage of incorrect mixtures and quantities of spices.<br />
<br />
<br />
''Brainstorm of Soil Mapping''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
"Space Dusters" / Space Crashers = Mediorite Landing<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
Tracing the path of Ganga<br />
<br />
Zoology of the Future<br />
Junkyard is separated out by object - sample each site<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
Defense Areas / BSF DRDO Air Force<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
Deadly Differences<br />
Samples from different types'/societies'/religions' burial grounds/crematoriums<br />
<br />
Soil "Classes"<br />
Soils from different classes of places<br />
"Holy", Royal, "Dirty", Common, etc<br />
<br />
Life Soil<br />
Trace all locations in a persons life<br />
Birth, Education, Settlement, Entertainment, Employment, Retirement, Health, Death, Graveyard, Paranormal activity<br />
<br />
<br />
<html><br />
<br />
<br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-05T20:58:39Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
One of our main aspects of doing igem everyear is making our own biolab from scrap, i.e jugaad.<br />
Using alternatives and different methods to carry out a completely different purpose. <br />
We started off with building a microscope from a webcam, and soon we were making a centrifuge out of a hand blender. We also made a laminar air flow, a desicator and an incubator from different materials.<br />
We built our own lab by constructing a geodesic 10 feet metal dome which we covered with tarp.<br />
<br />
Featured in this image is our own DIY microscope, sterile hood, incubator and a geodesic Biodome which houses our entire lab.<br />
<br />
<br />
[[File:collage1.jpg|350px]]<br />
<br />
<br />
[[File:collage2.jpg|350px]]<br />
<br />
<br />
[[File:collage3.jpg|350px]]<br />
<br />
<br />
[[File:collage4.jpg|350px]]<br />
<br />
<br />
[[File:collage5.jpg|350px]]<br />
<br />
<br />
[[File:collage7.jpg]]<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the guidance of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
Before our expeditions to collect soil samples, we came up with a tool kit that holds all the essentials required to be carried on the field while working:<br />
<br />
<br />
'''The First Prototype:'''<br />
<br />
[[File:collage8.jpg|350px]]<br />
<br />
<br />
<br />
<br />
'''Fold-able cloth toolkit'''<br />
<br />
[[File:collage9.jpg|350px]]<br />
<br />
<br />
<br />
'''Slab to store Epindorf tubes after soil sampling:'''<br />
<br />
[[File:collage10.jpg|350px]]<br />
<br />
<br />
<br />
'''Epindorf Bag'''<br />
<br />
[[File:collage11.jpg|350px]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with Friends'''<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
Shivers (a movie directed by David Cronenberg) is about a doctor’s attempts to bring out the inner sexual animal in man, using a parasite. He fooled his co-workers, and sponsors, into thinking that he was working on creating an alternative to organ transplant, by using parasites. The doctor was unable to accurately predict what the modified parasite would do. This lead to the parasite spreading from his teenaged mistress to quite a few other residents of a “sterile” high-end apartment block, and spreading uncontrollable sexual desire.<br />
This movie highlighted the unpredictable nature of the effects, the consequences of any modifications that we make in any organisms. It also highlights the unpredictability of the behaviour of the organisms. The behaviour of the modified organism in the laboratory is not adequate enough to predict its behaviour in the outside (real) world. The parasites modified an entire society and, in a manner, the entire purpose of a community.<br />
<br />
We also watched The Fly...and here are a few of the reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
Spices like chillies & pepper feel "hot" beacuse they contain certain chemicals like capsaicin and piperine. Our body (mainly our mouth) feels a sensation of burning pain, as a result of these chemicals. Our sensory neurons contain a functional cDNA encoding for a capsaicin receptor. The receptor is a non-selective cation channel that is also activated by increases in temperature. Similarly, other spices will all have acting chemicals, mostly organic. Each spice will also have corresponding receptors, or mechanisms, to induce responses. Most of the spices and condiments used are plant parts. This implies that they do contain cells (living or dead. Cells contain DNA, which would be responsible for the production of the chemicals responsible for the flavours of the spices. We would be able to identify the exact DNA sequences that code for the necessary chemicals by DNA sequencing. Once the sequences are identified, they can be introduced into the DNA of a suitable, safe bacteria. This bacteria would produce the different chemicals (for the spices) in certain fixed quantities when introduced into the food. This would reduce the chances of dishes getting "spoilt" due to the usage of incorrect mixtures and quantities of spices.<br />
<br />
<br />
''Brainstorm of Soil Mapping''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
"Space Dusters" / Space Crashers = Mediorite Landing<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
Tracing the path of Ganga<br />
<br />
Zoology of the Future<br />
Junkyard is separated out by object - sample each site<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
Defense Areas / BSF DRDO Air Force<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
Deadly Differences<br />
Samples from different types'/societies'/religions' burial grounds/crematoriums<br />
<br />
Soil "Classes"<br />
Soils from different classes of places<br />
"Holy", Royal, "Dirty", Common, etc<br />
<br />
Life Soil<br />
Trace all locations in a persons life<br />
Birth, Education, Settlement, Entertainment, Employment, Retirement, Health, Death, Graveyard, Paranormal activity<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
<!-- Content ends --><br />
</div><br />
<!-- Container ends --><br />
</html></div>Achalahttp://2011.igem.org/File:Collage9.jpgFile:Collage9.jpg2011-10-05T20:51:04Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-05T20:49:57Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
Featured in this image is our own DIY microscope, sterile hood, incubator and a geodesic Biodome which houses our entire lab.<br />
<br />
<br />
[[File:collage1.jpg|350px]]<br />
<br />
<br />
[[File:collage2.jpg|350px]]<br />
<br />
<br />
[[File:collage3.jpg|350px]]<br />
<br />
<br />
[[File:collage4.jpg|350px]]<br />
<br />
<br />
[[File:collage5.jpg|350px]]<br />
<br />
<br />
[[File:collage7.jpg]]<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
<br />
[[File:Lab_NCBS.jpg]]<br />
<br />
We worked in the labs at NCBS, under the guidance of Navneet Rai.<br />
<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
<br />
[[File:collage8.jpg|350px]]<br />
<br />
<br />
[[File:collage9.jpg|350px]]<br />
<br />
<br />
<br />
[[File:collage10.jpg|350px]]<br />
<br />
<br />
<br />
[[File:collage11.jpg|350px]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with Friends'''<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
Shivers (a movie directed by David Cronenberg) is about a doctor’s attempts to bring out the inner sexual animal in man, using a parasite. He fooled his co-workers, and sponsors, into thinking that he was working on creating an alternative to organ transplant, by using parasites. The doctor was unable to accurately predict what the modified parasite would do. This lead to the parasite spreading from his teenaged mistress to quite a few other residents of a “sterile” high-end apartment block, and spreading uncontrollable sexual desire.<br />
This movie highlighted the unpredictable nature of the effects, the consequences of any modifications that we make in any organisms. It also highlights the unpredictability of the behaviour of the organisms. The behaviour of the modified organism in the laboratory is not adequate enough to predict its behaviour in the outside (real) world. The parasites modified an entire society and, in a manner, the entire purpose of a community.<br />
<br />
We also watched The Fly...and here are a few of the reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
Spices like chillies & pepper feel "hot" beacuse they contain certain chemicals like capsaicin and piperine. Our body (mainly our mouth) feels a sensation of burning pain, as a result of these chemicals. Our sensory neurons contain a functional cDNA encoding for a capsaicin receptor. The receptor is a non-selective cation channel that is also activated by increases in temperature. Similarly, other spices will all have acting chemicals, mostly organic. Each spice will also have corresponding receptors, or mechanisms, to induce responses. Most of the spices and condiments used are plant parts. This implies that they do contain cells (living or dead. Cells contain DNA, which would be responsible for the production of the chemicals responsible for the flavours of the spices. We would be able to identify the exact DNA sequences that code for the necessary chemicals by DNA sequencing. Once the sequences are identified, they can be introduced into the DNA of a suitable, safe bacteria. This bacteria would produce the different chemicals (for the spices) in certain fixed quantities when introduced into the food. This would reduce the chances of dishes getting "spoilt" due to the usage of incorrect mixtures and quantities of spices.<br />
<br />
<br />
''Brainstorm of Soil Mapping''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
"Space Dusters" / Space Crashers = Mediorite Landing<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
Tracing the path of Ganga<br />
<br />
Zoology of the Future<br />
Junkyard is separated out by object - sample each site<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
Defense Areas / BSF DRDO Air Force<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
Deadly Differences<br />
Samples from different types'/societies'/religions' burial grounds/crematoriums<br />
<br />
Soil "Classes"<br />
Soils from different classes of places<br />
"Holy", Royal, "Dirty", Common, etc<br />
<br />
Life Soil<br />
Trace all locations in a persons life<br />
Birth, Education, Settlement, Entertainment, Employment, Retirement, Health, Death, Graveyard, Paranormal activity<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
<!-- Content ends --><br />
</div><br />
<!-- Container ends --><br />
</html></div>Achalahttp://2011.igem.org/File:Collage11.jpgFile:Collage11.jpg2011-10-05T20:48:42Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Collage10.jpgFile:Collage10.jpg2011-10-05T20:46:05Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Collage8.jpgFile:Collage8.jpg2011-10-05T20:42:34Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-05T20:39:48Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
<br />
<br />
[[File:collage1.jpg|350px]]<br />
<br />
<br />
[[File:collage2.jpg|350px]]<br />
<br />
<br />
[[File:collage3.jpg|350px]]<br />
<br />
<br />
[[File:collage4.jpg|350px]]<br />
<br />
<br />
[[File:collage5.jpg|350px]]<br />
<br />
<br />
[[File:collage7.jpg]]<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
<br />
[[File:collage8.jpg]]<br />
<br />
<br />
[[File:collage9.jpg]]<br />
<br />
<br />
<br />
[[File:collage10.jpg]]<br />
<br />
<br />
<br />
[[File:collage11.jpg]]<br />
<br />
<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
[[Image: kit1.JPG | x250px ]] [[Image: kit2.JPG | x250px ]] [[Image: kit3.JPG | x250px ]] [[Image: kit4.JPG | x250px ]] [[Image: kit5.JPG | x250px ]]<br />
<br />
Sidharta poses with his Soil Sampling toolkit<br />
<br />
<br />
<br />
<br />
<br />
'''Outreach with Friends'''<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
Shivers (a movie directed by David Cronenberg) is about a doctor’s attempts to bring out the inner sexual animal in man, using a parasite. He fooled his co-workers, and sponsors, into thinking that he was working on creating an alternative to organ transplant, by using parasites. The doctor was unable to accurately predict what the modified parasite would do. This lead to the parasite spreading from his teenaged mistress to quite a few other residents of a “sterile” high-end apartment block, and spreading uncontrollable sexual desire.<br />
This movie highlighted the unpredictable nature of the effects, the consequences of any modifications that we make in any organisms. It also highlights the unpredictability of the behaviour of the organisms. The behaviour of the modified organism in the laboratory is not adequate enough to predict its behaviour in the outside (real) world. The parasites modified an entire society and, in a manner, the entire purpose of a community.<br />
<br />
We also watched The Fly...and here are a few of the reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
Spices like chillies & pepper feel "hot" beacuse they contain certain chemicals like capsaicin and piperine. Our body (mainly our mouth) feels a sensation of burning pain, as a result of these chemicals. Our sensory neurons contain a functional cDNA encoding for a capsaicin receptor. The receptor is a non-selective cation channel that is also activated by increases in temperature. Similarly, other spices will all have acting chemicals, mostly organic. Each spice will also have corresponding receptors, or mechanisms, to induce responses. Most of the spices and condiments used are plant parts. This implies that they do contain cells (living or dead. Cells contain DNA, which would be responsible for the production of the chemicals responsible for the flavours of the spices. We would be able to identify the exact DNA sequences that code for the necessary chemicals by DNA sequencing. Once the sequences are identified, they can be introduced into the DNA of a suitable, safe bacteria. This bacteria would produce the different chemicals (for the spices) in certain fixed quantities when introduced into the food. This would reduce the chances of dishes getting "spoilt" due to the usage of incorrect mixtures and quantities of spices.<br />
<br />
<br />
''Brainstorm of Soil Mapping''<br />
<br />
Urban Bacterial Theater<br />
<br />
Desire Line Drawing<br />
Capture the paths of people on a park using Stop Motion<br />
Sample along those paths<br />
<br />
Hospital Waste Samples<br />
Human Parts thrown out back?<br />
Nurse Costume<br />
<br />
Family History<br />
<br />
Super Hero<br />
Saving the Day<br />
Radiation / Mutation i.e. Spiderman, Incredible Hulk<br />
<br />
Disaster Mapping<br />
Fukishima, Chernobyl, Bhopal etc...<br />
E-Waste Sites<br />
<br />
"Space Dusters" / Space Crashers = Mediorite Landing<br />
<br />
Retracing a walk<br />
Recursion / Remembering<br />
Gandhi Sea March<br />
Silk Road<br />
Ghengis Kahn<br />
<br />
Topography / BioTrekking<br />
<br />
Farmer / Drought / IR Mapping<br />
<br />
Costume = Bullet Belt<br />
<br />
Blood Soil<br />
Conflict Areas / Borders<br />
Israel / Palestine<br />
Kashmir<br />
Tropic of Cancer - Bhuj<br />
<br />
Tea Plantation<br />
Basket Costume for Collecting samples<br />
Terroir<br />
<br />
Ant Hills / Termite Mounds<br />
Costume = A Bacteria Zoologist / Arcehologist<br />
<br />
Paranormal Activity<br />
Soil Ghost Hunters<br />
<br />
Soul in the Soil<br />
Graveyard / Tower of Silence<br />
<br />
Sending Soil on a Journey<br />
Put a potted plant on a train and take samples at each end<br />
<br />
Soil Sadu<br />
Ask for soil samples instead of alms<br />
Wiseman / karma<br />
<br />
Culture Soil<br />
Compare different cultures / paint clothes<br />
<br />
National PCR<br />
Compare national data (GDP, GINI) with PCR data<br />
<br />
Sunrise Sampling<br />
Grab samples as the sun comes up across the world<br />
<br />
Airplane dust<br />
Collect samples on the plane<br />
<br />
Sea beds<br />
Since it is no ones land. International waters<br />
<br />
Oil Refinery / Shipping Ports<br />
Look for oil contamination / Map the flow of energy<br />
<br />
Shadows & Soils<br />
Micro-climates on ONE site based on where sun hits or doesn't<br />
<br />
Tracing the path of Ganga<br />
<br />
Zoology of the Future<br />
Junkyard is separated out by object - sample each site<br />
<br />
Old vs New<br />
Old vs. New Airport in BLR<br />
Oldest Building in BLR and site that is being built on now<br />
Schools vs. Old Age Homes<br />
<br />
Royal Soil Difference between "holy" soil & normal soil<br />
<br />
Neighborhood Soil<br />
<br />
Cultural Soils<br />
<br />
Walking / Imagery of Feet<br />
<br />
Defense Areas / BSF DRDO Air Force<br />
<br />
Lake / Garbage Disposal<br />
<br />
Evolution<br />
Mapping the evolution of borders/city limits<br />
Soil samples along different border<br />
<br />
Trace a day<br />
Follow a cow/street dog/goat across the city, and take soil samples along the path say, every 30 mins.<br />
Follow people from "contrasting" walks of life<br />
<br />
Deadly Differences<br />
Samples from different types'/societies'/religions' burial grounds/crematoriums<br />
<br />
Soil "Classes"<br />
Soils from different classes of places<br />
"Holy", Royal, "Dirty", Common, etc<br />
<br />
Life Soil<br />
Trace all locations in a persons life<br />
Birth, Education, Settlement, Entertainment, Employment, Retirement, Health, Death, Graveyard, Paranormal activity<br />
<br />
<br />
<html><br />
<br />
<br />
<br />
<br />
</div><br />
<!-- Content ends --><br />
</div><br />
<!-- Container ends --><br />
</html></div>Achalahttp://2011.igem.org/File:Collage6.jpgFile:Collage6.jpg2011-10-05T20:23:41Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Collage5.jpgFile:Collage5.jpg2011-10-05T20:16:38Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Collage4.jpgFile:Collage4.jpg2011-10-05T20:11:32Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Collage2.jpgFile:Collage2.jpg2011-10-05T20:08:07Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Collage3.jpgFile:Collage3.jpg2011-10-05T20:05:30Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Collage1.jpgFile:Collage1.jpg2011-10-05T19:50:35Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/NotebookTeam:ArtScienceBangalore/Notebook2011-10-05T19:49:00Z<p>Achala: </p>
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<br />
'''Building our own BioLab'''<br />
[[File:collage1.jpg|350px]]<br />
[[File:collage2.jpg|350px]]<br />
[[File:collage3.jpg|350px]]<br />
[[File:collage4.jpg|350px]]<br />
[[File:collage5.jpg|350px]]<br />
[[File:collage6.jpg|350px]]<br />
<br />
<br />
'''Experiments at NCBS'''<br />
<br />
'''The BioGeographer's toolkit'''<br />
<br />
'''The Post-Natural BioGeographer'''<br />
<br />
'''Outreach with Friends'''<br />
<br />
'''Watching some David Cronenberg'''<br />
<br />
Shivers (a movie directed by David Cronenberg) is about a doctor’s attempts to bring out the inner sexual animal in man, using a parasite. He fooled his co-workers, and sponsors, into thinking that he was working on creating an alternative to organ transplant, by using parasites. The doctor was unable to accurately predict what the modified parasite would do. This lead to the parasite spreading from his teenaged mistress to quite a few other residents of a “sterile” high-end apartment block, and spreading uncontrollable sexual desire.<br />
This movie highlighted the unpredictable nature of the effects, the consequences of any modifications that we make in any organisms. It also highlights the unpredictability of the behaviour of the organisms. The behaviour of the modified organism in the laboratory is not adequate enough to predict its behaviour in the outside (real) world. The parasites modified an entire society and, in a manner, the entire purpose of a community.<br />
<br />
We also watched The Fly...and here are a few of the reactions!<br />
<br />
<br />
[[File:Watching The Fly.jpg]]<br />
<br />
<br />
<br />
'''Some Early Speculative ideas'''<br />
<br />
<br />
[[File:Spicy_Bacteria.jpg]]<br />
<br />
Spices like chillies & pepper feel "hot" beacuse they contain certain chemicals like capsaicin and piperine. Our body (mainly our mouth) feels a sensation of burning pain, as a result of these chemicals. Our sensory neurons contain a functional cDNA encoding for a capsaicin receptor. The receptor is a non-selective cation channel that is also activated by increases in temperature. Similarly, other spices will all have acting chemicals, mostly organic. Each spice will also have corresponding receptors, or mechanisms, to induce responses. Most of the spices and condiments used are plant parts. This implies that they do contain cells (living or dead. Cells contain DNA, which would be responsible for the production of the chemicals responsible for the flavours of the spices. We would be able to identify the exact DNA sequences that code for the necessary chemicals by DNA sequencing. Once the sequences are identified, they can be introduced into the DNA of a suitable, safe bacteria. This bacteria would produce the different chemicals (for the spices) in certain fixed quantities when introduced into the food. This would reduce the chances of dishes getting "spoilt" due to the usage of incorrect mixtures and quantities of spices.<br />
<br />
<html><br />
<br />
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'''Abstract'''<br />
<br />
The BioBrick has been used as an abstraction or template for creating<br />
standardized functional parts. This year's ArtScienceBangalore project<br />
proposes alternate re-appropriations of the BioBrick by using existing<br />
BioBrick primers as random-PCR primers in investigating soil samples.<br />
These random PCR primers provide a succinct signature of the<br />
biological diversity present in soil samples. Such investigation of<br />
soil leads us to ask questions about citizen’s science "performed" by<br />
non-institutional actors using accessible tools, and also offers a<br />
glimpse into the "post-natural world" where BioBricks may very well<br />
end up in our environment as bands in a gel. By imagining a world in<br />
which the BioBrick has become the accepted standard for synthetic<br />
biology, and where these engineered products are ubiquitous in our<br />
lives and environments, the samples we have archived will serve as the<br />
baseline from which the subsequent extent of human influence can be<br />
measured.<br />
<br />
Our project is part speculative. Would it be possible to involve a<br />
larger community in a Synthetic Biology project? If yes, what<br />
questions would we collectively ask and how would we find answers to<br />
these questions together? If we could create an open community of<br />
citizen scientists by initiating such investigations in an inexpensive<br />
manner, could science become a performative celebration of humanity?<br />
<br />
<br />
'''The Project'''<br />
<br />
Can you repurpose the BioBrick to use it for environmental mapping? What will happen when results from many IGEM projects enter the real world? Would they show up as bands in a gel? <br />
<br />
We explored answers to these questions through the following: <br />
<br />
Collecting soil samples from contested spaces i.e. environments with high levels of pesticides, background radiation, and chemical pollution<br />
<br />
Mapping the various contested spaces from where these soil samples were collected, using grassroots mapping techniques like helium balloon mapping<br />
<br />
Extracting DNA from the soil samples and creating an archive of these samples<br />
<br />
Amplifying the DNA using BioBricks as PCR primers <br />
<br />
Sharing the PCR gel results of the soil samples on a publically accessible online map<br />
<br />
Inviting people to send in their soil samples to participate in a public science-art project<br />
<br />
<br />
[[File:Illustration_from_Sidharta.jpg]]<br />
<br />
<br />
'''Open Citizen’s Science'''<br />
<br />
Instead of creating BioBricks, as is the IGEM project norm, we focussed on creating novel ways for existing BioBricks to be used by ordinary citizen scientists to investigate their neighborhoods. One of the areas that we chose to focus on was microbial diversity in soil. However, the use of expensive genetic sequencers was out of the question. So we explored a simple way to analyze diversity .i.e. compare PCR gel bands from DNA extracted from various soil samples and use that to compare soil microbial diversity. <br />
<br />
<br />
'''Simple hacks for the BioBrick'''<br />
<br />
Since BioBricks are easily available, we used them as primers for PCR. <br />
This solves 2 issues: <br />
You don’t have to buy primers<br />
You can easily map synthetic organisms in the environment (if you find any)<br />
<br />
<br />
'''Grassroot mapping of Soil Diversity'''<br />
<br />
We also borrowed some techniques of [http://publiclaboratory.org/tool/balloon-mapping grassroot cartographers] to build our own maps of the soil samples and map GEL bands on to them. We hope to expand this project into a public science project.<br />
<br />
<br />
<br />
== Project Process and Protocols ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
We collected various soil samples from which we extracted DNA and ran it through a PCR machine (using standard Biobrick primers) followed by gel electrophoresis to characterize the combined flora and fauna of a site visually; the ecology’s fingerprint, if you will. <br />
<br />
Later we compared the results from various samples in an attempt to find correlations.<br />
<br />
Each soil site was documented through aerial photography. This was achieved by the use of a handmade helium-filled balloon mount and a camera. The photographs from these were sown together to produce a comprehensive map of the immediate surroundings.<br />
<br />
<br />
'''Extraction of DNA from soil samples'''<br />
<br />
<br />
[[File:DNA_extraction.jpg]]<br />
<br />
<br />
''Protocol 1''<br />
<br />
1. Before the DNA can be extracted the cell walls must be broken open. This process is called lysing and the agent used is called a lysosome. We did this by vortexing using phenol-chloroform, and further the addition of SDS (a detergent) to remove the membranes of lipids.<br />
<br />
2. Next comes the centrifuging to separate the layers. The DNA lies in the interface between the two phases (proteins and reagents).<br />
<br />
3. After the protein is removed the DNA can be precipitated using cold ethanol or isopropanol (propan-2-ol). The DNA, being insoluble in alcohol, will come out of the solution. Additionally the alcohol serves as a wash to remove the added salts.<br />
<br />
4. The resulting DNA pellet should be washed with cold ethanol and centrifuged.<br />
<br />
5. After drying the pellet the DNA should be resuspended in a Tris buffer solution.<br />
<br />
6. Presence of DNA can be confirmed by electrophoresing on an agarose gel containing ethidium bromide, or another fluorescent dye that reacts with the DNA, and checking under UV light.<br />
<br />
<br />
<br />
''Protocol 2''<br />
<br />
1. For dissolving the DNA present in the soil, mix the soil sample (5 g) in Tris Buffer (5 mL of 25 mM buffer) and mix thoroughly.<br />
<br />
2. Then in order to separate the cells out of the solution, filter using 5μm cellulose nitrate filter. Most microbial cells are between 0μm & 5μm, and will be a part of the filtrate.<br />
<br />
3. In order to separate the different components of the filtrate, it is centrifuged at 13.2 krpm for 10 mins at room temperature(25°C).<br />
<br />
4. The pellet, containing the concentrated DNA (after centrifugation) is separated from the supernatant, and dissolved in 100μL autoclaved milliQ water.<br />
<br />
5. The resultant mixture is boiled at 100°C for 20 minutes.<br />
<br />
6. Presence of DNA can be confirmed by electrophoresing on an agarose gel containing ethidium bromide, or another fluorescent dye that reacts with the DNA, and checking under UV light.<br />
<br />
<br />
''Protocol 3''<br />
<br />
1. For dissolving the DNA present in the soil, mix the soil sample in Tris Buffer (2 gm soil sample in 2 mL 25mM Tris Buffer) and mix thoroughly.<br />
<br />
2. Freeze the eppendorf tubes containing the above mixture in liquid nitrogen, and then boil (at 100°C for 20 mins). This will cause breakdown of the cell, and release the DNA into the mixture.<br />
<br />
3. It is then centrifuged at 13.2 krpm for 10 mins at 25°C. This will result in the separation of the DNA from the soil particles.<br />
<br />
4. Discard the soil particles, and collect the supernatant.<br />
<br />
5. Presence of DNA can be confirmed by electrophoresing on an agarose gel containing ethidium bromide, or another fluorescent dye that reacts with the DNA, and checking under UV light.<br />
<br />
<br />
<br />
'''Gel Electrophoresis'''<br />
<br />
Presence of DNA can be confirmed by electrophoresing on an agarose gel containing ethidium bromide, or another fluorescent dye that reacts with the DNA, and checking under UV light.<br />
<br />
''Preparing an agarose gel solution''<br />
<br />
1. Add the tris buffer to the agarose powder, heat at 50 degrees centigrade till you obtain a clear solution and add Ethidium bromide.<br />
<br />
2. Pour into the Gel box, add the comb to form wells, get rid of any air bubbles and let it settle for 30 minutes.<br />
<br />
3. Load 3 micro litres of the samples in the wells <br />
<br />
4. Begin Gel electrophoresis<br />
<br />
<br />
'''Gel Bands from the rain forests of Coorg'''<br />
<br />
<br />
'''Building a Balloon Mapper'''<br />
<br />
<br />
'''A Sample DIY Map'''<br />
<br />
[[File:map.jpg]]<br />
<br />
<br />
'''Use [http://www.google.com/fusiontables/public/tour/index.html Google Fusion Tables] to Plot Gel Bands geographically'''<br />
<br />
<iframe width="500px" height="300px" scrolling="no" src="http://www.google.com/fusiontables/embedviz?viz=MAP&q=select+col2+from+1741275+&h=false&lat=12.679195536451866&lng=75.8503510055176&z=10&t=4&l=col2"></iframe><br />
<br />
<websiteFrame><br />
website=http://www.google.com<br />
height=800<br />
width=100%<br />
border=0<br />
scroll=yes<br />
</websiteFrame><br />
<br />
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Art Science Bangalore is a team of 12, Core Skills (2nd year) Art & Design students from Srishti School of Art, Design and Technology, Bangalore. Yashas Shetty, the team facilitator, and Aaron Joseph the team advisor, led the team through the project and research along with the help of Mukund Tathattai,Zack Denfeld & Cathrine Kramer . Special thanks to the scientists at National Centre of Biological Sciences (NCBS) – Mukund Tathattai, Ashwin Seshasayee and Navneet Rai for helping us out with the experiments and lab work.<br />
<br />
The profile of each team member is mentioned below:<br />
<br />
<br />
<CENTER>[[File:teamcollage.jpg|500px]]</CENTER><br />
<br />
<br />
'''Achala Athreya''' <br />
<br />
After joining an art school, Achala never imagined herself ever taking part in a science based competition. The recurring field in the second year of her college life inculcated future design inklings. Her interests however, lean towards textiles, photography and theatre. She enjoys tennis and lately is a maniac about yoga.<br />
<br />
<br />
'''Akshaya Maria Thomas'''<br />
<br />
Easily recognizable with her wild curly hair, Akshaya is a keen learner and a photographer at heart. She likes experimenting with new methods and techniques. Stationary makes her happy. She enjoys baking and playing chess. She detests clowns and things that crawl.<br />
<br />
<br />
'''Anvita Goenka'''<br />
<br />
Anvita never had interest in biology or any kind of science from her school days and decided to try something different and that is how she landed up in Art Science Bangalore. Apart from working on this project, her interests are mythology, catching up with new trends of fashion, experimenting with looks and clothes, food and wildlife.<br />
<br />
<br />
'''Biswajit Manimaran'''<br />
<br />
When you say “Bisu”, his obsession with video games, his laptop, and green shirts are a few things that springs to one’s mind. He’s the “go-to” person for explaining the huge scientific terms that most commoners shy away from, and for explaining most technological terms. He’s quite naturally the resident geek of Art Science Bangalore.<br />
<br />
<br />
'''Damyanti Choudhary'''<br />
<br />
After the joyful moment of passing her science exam for the first time, Damyanti hasn’t looked back at science as a subject since her 10th grade. Through her years since then, her interests developed in interior design, which also led her to join art school. But now, she contemplates the thoughts of pursuing product design, which also became one of her major interests. She’s loud and very short tempered, but has got the perfect skill for organization and research.<br />
<br />
<br />
'''Durga Gawde'''<br />
<br />
Born and raised in an art environment, Durga always knew what her calling was. Science always intrigued her. Although, she hated the way science was taught in the school level her love for science never left her. Igem helped her see new methods of learning, understanding and application. Apart from working on this project, her interests are sports (mainly speed skating), experimenting with photography, listening to powerful music, conversing with different types of people and working out. <br />
<br />
<br />
'''Madhul Singh'''<br />
<br />
Madhul spends her time playing computer games and stumbling on StumbleUpon. An avid reader, she has a special fascination for mythology and wildlife. She hopes to pursue both at some point. She also has a fascination for adventure, and climbs a rock-face like a goat, though coming down is a whole different ballgame (she has Vertigo!). She's the first person you go to when you're troubled and the last person you'd want trouble with. Beware!<br />
<br />
<br />
'''Manasa Thimmiya'''<br />
<br />
Manasa loves music, and travel. Her love for new things and her passion for communication design are what pushed her to do iGem. She is notorious for her brilliant graphics, her interest in social service, and her laziness. <br />
<br />
<br />
'''Prakrithy Pradeep'''<br />
<br />
Prakrithy loves to read (everything and anything, except self-help books - they are just plain "Blah") and makes biryani (a preparation of a one-pot meal with rice and meat, laced with exotic spices) to die for. She spends her free time indulging in writing and photography. She dislikes cockroaches and people eating her chocolate, and indulges in using her (always-carry-with-you) Post-Its for anything and everything.<br />
<br />
<br />
'''Pranav Raman'''<br />
<br />
From a very young age Pranav was always interested in science and its applications. Being an art student he has channelized his attempts at finding a harmonious combination of both science and design. It has always fascinated him how if put together, they have done wonders. Looking at science through an artist’s point of view has opened up a whole new world to him. He also has a keen interest music, singing, playing the tabla and flute.<br />
<br />
<br />
'''Sohil Bhatia'''<br />
<br />
Famous as “safety pin boy” and for his wild and crazy ideas-when it comes to his sense of dressing and lifestyle, Sohil brings the crazy side out in Art Science Bangalore. His work ethic and dedication to any project is influenced by his idol- Lady Gaga. His interests include drama, photography and product design.<br />
<br />
<br />
'''Sidharta Guha Thakurta''' <br />
<br />
The “free spirited” one of the team, Sidartha is a born artist who is into expressionist sculpting and painting. He’s enigmatic and has a wild sense of humour and culinary preferences.<br />
<br />
<br />
<br />
== Special Thanks ==<br />
'''Samuel Rajkumar'''(Srishti School of Art, Design and Technology)<br />
'''Ajai Narendran'''(Srishti School of Art, Design and Technology)<br />
'''Waseem Sheikh'''(Srishti School of Art, Design and Technology)<br />
'''Ramesh Kalkur'''(Srishti School of Art, Design and Technology)<br />
'''Geetha Narayanan''' (Srishti School of Art, Design and Technology)<br />
<br />
'''Sujata Goel'''(Rainforest Retreat, Coorg)<br />
'''Anurag Goel'''(Rainforest Retreat, Coorg)<br />
and '''Archana'''(Rainforest Retreat, Coorg)<br />
<br />
'''Hackteria.org'''<br />
<br />
<br />
ArtScienceBLR is supported by the Srishti School of Art,Design and Technology, Bangalore and the National Centre for Biological Scienes, Bangalore.<br />
<br />
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Art Science Bangalore is a team of 12, Core Skills (2nd year) Art & Design students from Srishti School of Art, Design and Technology, Bangalore. Yashas Shetty, the team facilitator, and Aaron Joseph the team advisor, led the team through the project and research along with the help of Zack Denfeld & Cathrine Kramer . Special thanks to the scientists at National Centre of Biological Sciences (NCBS) – Mukund Tathattai, Ashwin Seshasayee and Navneet Rai for helping us out with the experiments and lab work.<br />
<br />
The profile of each team member is mentioned below:<br />
<br />
<br />
<CENTER>[[File:teamcollage.jpg|500px]]</CENTER><br />
<br />
<br />
'''Achala Athreya''' <br />
<br />
After joining an art school, Achala never imagined herself ever taking part in a science based competition. The recurring field in the second year of her college life inculcated future design inklings. Her interests however, lean towards textiles, photography and theatre. She enjoys tennis and lately is a maniac about yoga.<br />
<br />
<br />
'''Akshaya Maria Thomas'''<br />
<br />
Easily recognizable with her wild curly hair, Akshaya is a keen learner and a photographer at heart. She likes experimenting with new methods and techniques. Stationary makes her happy. She enjoys baking and playing chess. She detests clowns and things that crawl.<br />
<br />
<br />
'''Anvita Goenka'''<br />
<br />
Anvita never had interest in biology or any kind of science from her school days and decided to try something different and that is how she landed up in Art Science Bangalore. Apart from working on this project, her interests are mythology, catching up with new trends of fashion, experimenting with looks and clothes, food and wildlife.<br />
<br />
<br />
'''Biswajit Manimaran'''<br />
<br />
When you say “Bisu”, his obsession with video games, his laptop, and green shirts are a few things that springs to one’s mind. He’s the “go-to” person for explaining the huge scientific terms that most commoners shy away from, and for explaining most technological terms. He’s quite naturally the resident geek of Art Science Bangalore.<br />
<br />
<br />
'''Damyanti Choudhary'''<br />
<br />
After the joyful moment of passing her science exam for the first time, Damyanti hasn’t looked back at science as a subject since her 10th grade. Through her years since then, her interests developed in interior design, which also led her to join art school. But now, she contemplates the thoughts of pursuing product design, which also became one of her major interests. She’s loud and very short tempered, but has got the perfect skill for organization and research.<br />
<br />
<br />
'''Durga Gawde'''<br />
<br />
Born and raised in an art environment, Durga always knew what her calling was. Science always intrigued her. Although, she hated the way science was taught in the school level her love for science never left her. Igem helped her see new methods of learning, understanding and application. Apart from working on this project, her interests are sports (mainly speed skating), experimenting with photography, listening to powerful music, conversing with different types of people and working out. <br />
<br />
<br />
'''Madhul Singh'''<br />
<br />
Madhul spends her time playing computer games and stumbling on StumbleUpon. An avid reader, she has a special fascination for mythology and wildlife. She hopes to pursue both at some point. She also has a fascination for adventure, and climbs a rock-face like a goat, though coming down is a whole different ballgame (she has Vertigo!). She's the first person you go to when you're troubled and the last person you'd want trouble with. Beware!<br />
<br />
<br />
'''Manasa Thimmiya'''<br />
<br />
Manasa loves music, and travel. Her love for new things and her passion for communication design are what pushed her to do iGem. She is notorious for her brilliant graphics, her interest in social service, and her laziness. <br />
<br />
<br />
'''Prakrithy Pradeep'''<br />
<br />
Prakrithy loves to read and write (everything and anything, except self-help books - they are just plain "Blah") and makes biryani (a preparation of a one-pot meal with rice and meat, laced with exotic spices) to die for. She spends her free time indulging in photography. She dislikes cockroaches and people eating her chocolate, and indulges in using her (always-carry-with-you) Post-Its for anything and everything.<br />
<br />
<br />
'''Pranav Raman'''<br />
<br />
From a very young age Pranav was always interested in science and its applications. Being an art student he has channelized his attempts at finding a harmonious combination of both science and design. It has always fascinated him how if put together, they have done wonders. Looking at science through an artist’s point of view has opened up a whole new world to him. He also has a keen interest music, singing, playing the tabla and flute.<br />
<br />
<br />
'''Sohil Bhatia'''<br />
<br />
Famous as “safety pin boy” and for his wild and crazy ideas-when it comes to his sense of dressing and lifestyle, Sohil brings the crazy side out in Art Science Bangalore. His work ethic and dedication to any project is influenced by his idol- Lady Gaga. His interests include drama, photography and product design.<br />
<br />
<br />
'''Sidharta Guha Thakurta''' <br />
<br />
The “free spirited” one of the team, Sidartha is a born artist who is into expressionist sculpting and painting. He’s enigmatic and has a wild sense of humour and culinary preferences.<br />
<br />
<br />
<br />
<br />
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Art Science Bangalore is a team of 12, Core Skills (2nd year) Art & Design students from Srishti School of Art, Design and Technology, Bangalore. Yashas Shetty, the team facilitator, and Aaron Joseph the team advisor, led the team through the project and research along with the help of Zack Denfeld & Cathrine Kramer . Special thanks to the scientists at National Centre of Biological Sciences (NCBS) – Mukund Tathattai, Ashwin Seshasayee and Navneet Rai for helping us out with the experiments and lab work.<br />
<br />
The profile of each team member is mentioned below:<br />
<br />
<br />
<CENTER>[[File:teamcollage.jpg|500px]]</CENTER><br />
<br />
<br />
'''Achala Athreya''' <br />
<br />
After joining an art school, Achala never imagined herself ever taking part in a science based competition. The recurring field in the second year of her college life inculcated future design inklings. Her interests however, lean towards textiles, photography and theatre. She enjoys tennis and lately is a maniac about yoga.<br />
<br />
<br />
'''Akshaya Maria Thomas'''<br />
<br />
Easily recognizable with her wild curly hair, Akshaya is a keen learner. A photographer at heart, she likes experimenting with new methods and techniques. Stationary makes her happy. She enjoys baking and playing chess. She detests clowns and things that crawl.<br />
<br />
<br />
'''Anvita Goenka'''<br />
<br />
Anvita never had interest in biology or any kind of science from her school days and decided to try something different and that is how she landed up in Art Science Bangalore. Apart from working on this project, her interests are mythology, catching up with new trends of fashion, experimenting with looks and clothes, food and wildlife.<br />
<br />
<br />
'''Biswajit Manimaran'''<br />
<br />
When you say “Bisu”, his obsession with video games, his laptop, and green shirts are a few things that springs to one’s mind. He’s the “go-to” person for explaining the huge scientific terms that most commoners shy away from, and for explaining most technological terms. He’s quite naturally the resident geek of Art Science Bangalore.<br />
<br />
<br />
'''Damyanti Choudhary'''<br />
<br />
After the joyful moment of passing her science exam for the first time, Damyanti hasn’t looked back at science as a subject since her 10th grade. Through her years since then, her interests developed in interior design, which also led her to join art school. But now, she contemplates the thoughts of pursuing product design, which also became one of her major interests. She’s loud and very short tempered, but has got the perfect skill for organization and research.<br />
<br />
<br />
'''Durga Gawde'''<br />
<br />
Born and raised in an art environment, Durga always knew what her calling was. Science always intrigued her. Although, she hated the way science was taught in the school level her love for science never left her. Igem helped her see new methods of learning, understanding and application. Apart from working on this project, her interests are sports (mainly speed skating), experimenting with photography, listening to powerful music, conversing with different types of people and working out. <br />
<br />
<br />
'''Madhul Singh'''<br />
<br />
Madhul spends her time playing computer games and stumbling on StumbleUpon. An avid reader, she has a special fascination for mythology and wildlife. She hopes to pursue both at some point. She also has a fascination for adventure, and climbs a rock-face like a goat, though coming down is a whole different ballgame (she has Vertigo!). She's the first person you go to when you're troubled and the last person you'd want trouble with. Beware!<br />
<br />
<br />
'''Manasa Thimmiya'''<br />
<br />
Manasa loves music, and travel. Her love for new things and her passion for communication design are what pushed her to do iGem. She is notorious for her brilliant graphics, her interest in social service, and her laziness. <br />
<br />
<br />
'''Prakrithy Pradeep'''<br />
<br />
Prakrithy loves to read and write (everything and anything, except self-help books - they are just plain "Blah") and makes biryani (a preparation of a one-pot meal with rice and meat, laced with exotic spices) to die for. She spends her free time indulging in photography. She dislikes cockroaches and people eating her chocolate, and indulges in using her (always-carry-with-you) Post-Its for anything and everything.<br />
<br />
<br />
'''Pranav Raman'''<br />
<br />
From a very young age Pranav was always interested in science and its applications. Being an art student he has channelized his attempts at finding a harmonious combination of both science and design. It has always fascinated him how if put together, they have done wonders. Looking at science through an artist’s point of view has opened up a whole new world to him. He also has a keen interest music, singing, playing the tabla and flute.<br />
<br />
<br />
'''Sohil Bhatia'''<br />
<br />
Famous as “safety pin boy” and for his wild and crazy ideas-when it comes to his sense of dressing and lifestyle, Sohil brings the crazy side out in Art Science Bangalore. His work ethic and dedication to any project is influenced by his idol- Lady Gaga. His interests include drama, photography and product design.<br />
<br />
<br />
'''Sidharta Guha Thakurta''' <br />
<br />
The “free spirited” one of the team, Sidartha is a born artist who is into expressionist sculpting and painting. He’s enigmatic and has a wild sense of humour and culinary preferences.<br />
<br />
<br />
<br />
<br />
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Art Science Bangalore is a team of 12, Core Skills (2nd year) Art & Design students from Srishti School of Art, Design and Technology, Bangalore. Yashas Shetty, the team facilitator, and Aaron Joseph the team advisor, led the team through the project and research along with the help of Zack Denfeld & Cathrine Kramer . Special thanks to the scientists at National Centre of Biological Sciences (NCBS) – Mukund Tathattai, Ashwin Seshasayee and Navneet Rai for helping us out with the experiments and lab work.<br />
<br />
The profile of each team member is mentioned below:<br />
<br />
<br />
<CENTER>[[File:teamcollage.jpg|500px]]</CENTER><br />
<br />
<br />
'''Achala Athreya''' <br />
<br />
After joining an art school, Achala never imagined herself ever taking part in a science based competition. The recurring field in the second year of her college life inculcated future design inklings. Her interests however, lean towards textiles, photography and theatre. She enjoys tennis and lately is a maniac about yoga.<br />
<br />
<br />
'''Akshaya Maria Thomas'''<br />
<br />
Easily recognisable because of her wild curly hair, Akshaya is a keen learner. A photographer at heart, she likes experimenting with new methods and techniques. Stationary makes her happy. She enjoys baking and playing chess. She detests clowns and things that crawl.<br />
<br />
<br />
'''Anvita Goenka'''<br />
<br />
Anvita never had interest in biology or any kind of science from her school days and decided to try something different and that is how she landed up in Art Science Bangalore. Apart from working on this project, her interests are mythology, catching up with new trends of fashion, experimenting with looks and clothes, food and wildlife.<br />
<br />
<br />
'''Biswajit Manimaran'''<br />
<br />
When you say “Bisu”, his obsession with video games, his laptop, and green shirts are a few things that springs to one’s mind. He’s the “go-to” person for explaining the huge scientific terms that most commoners shy away from, and for explaining most technological terms. He’s quite naturally the resident geek of Art Science Bangalore.<br />
<br />
<br />
'''Damyanti Choudhary'''<br />
<br />
After the joyful moment of passing her science exam for the first time, Damyanti hasn’t looked back at science as a subject since her 10th grade. Through her years since then, her interests developed in interior design, which also led her to join art school. But now, she contemplates the thoughts of pursuing product design, which also became one of her major interests. She’s loud and very short tempered, but has got the perfect skill for organization and research.<br />
<br />
<br />
'''Durga Gawde'''<br />
<br />
Born and raised in an art environment, Durga always knew what her calling was. Science always intrigued her. Although, she hated the way science was taught in the school level her love for science never left her. Igem helped her see new methods of learning, understanding and application. Apart from working on this project, her interests are sports (mainly speed skating), experimenting with photography, listening to powerful music, conversing with different types of people and working out. <br />
<br />
<br />
'''Madhul Singh'''<br />
<br />
Madhul spends her time playing computer games and stumbling on StumbleUpon. An avid reader, she has a special fascination for mythology and wildlife. She hopes to pursue both at some point. She also has a fascination for adventure, and climbs a rock-face like a goat, though coming down is a whole different ballgame (she has Vertigo!). She's the first person you go to when you're troubled and the last person you'd want trouble with. Beware!<br />
<br />
<br />
'''Manasa Thimmiya'''<br />
<br />
Manasa loves music, and travel. Her love for new things and her passion for communication design are what pushed her to do iGem. She is notorious for her brilliant graphics, her interest in social service, and her laziness. <br />
<br />
<br />
'''Prakrithy Pradeep'''<br />
<br />
Prakrithy loves to read and write (everything and anything, except self-help books - they are just plain "Blah") and makes biryani (a preparation of a one-pot meal with rice and meat, laced with exotic spices) to die for. She spends her free time indulging in photography. She dislikes cockroaches and people eating her chocolate, and indulges in using her (always-carry-with-you) Post-Its for anything and everything.<br />
<br />
<br />
'''Pranav Raman'''<br />
<br />
From a very young age Pranav was always interested in science and its applications. Being an art student he has channelized his attempts at finding a harmonious combination of both science and design. It has always fascinated him how if put together, they have done wonders. Looking at science through an artist’s point of view has opened up a whole new world to him. He also has a keen interest music, singing, playing the tabla and flute.<br />
<br />
<br />
'''Sohil Bhatia'''<br />
<br />
Famous as “safety pin boy” and for his wild and crazy ideas-when it comes to his sense of dressing and lifestyle, Sohil brings the crazy side out in Art Science Bangalore. His work ethic and dedication to any project is influenced by his idol- Lady Gaga. His interests include drama, photography and product design.<br />
<br />
<br />
'''Sidharta Guha Thakurta''' <br />
<br />
The “free spirited” one of the team, Sidartha is a born artist who is into expressionist sculpting and painting. He’s enigmatic and has a wild sense of humour and culinary preferences.<br />
<br />
<br />
<br />
<br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2011-10-05T08:10:04Z<p>Achala: </p>
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Art Science Bangalore is a team of 12, Core Skills (2nd year) Art & Design students from Srishti School of Art, Design and Technology, Bangalore. Yashas Shetty, the team facilitator, and Aaron Joseph the team advisor, led the team through the project and research along with the help of Zack Denfeld & Cathrine Kramer . Special thanks to the scientists at National Centre of Biological Sciences (NCBS) – Mukund Tathattai, Ashwin Seshasayee and Navneet Rai for helping us out with the experiments and lab work.<br />
<br />
The profile of each team member is mentioned below:<br />
<br />
<br />
[CENTER][[File:teamcollage.jpg|500px]][/CENTER]<br />
<br />
<br />
'''Achala Athreya''' <br />
<br />
After joining an art school, Achala never imagined herself ever taking part in a science based competition. The recurring field in the second year of her college life inculcated future design inklings. Her interests however, lean towards textiles, photography and theatre. She enjoys tennis and lately is a maniac about yoga.<br />
<br />
<br />
'''Akshaya Maria Thomas'''<br />
<br />
Easily recognisable because of her wild curly hair, Akshaya is a keen learner. A photographer at heart, she likes experimenting with new methods and techniques. Stationary makes her happy. She enjoys baking and playing chess. She detests clowns and things that crawl.<br />
<br />
<br />
'''Anvita Goenka'''<br />
<br />
Anvita never had interest in biology or any kind of science from her school days and decided to try something different and that is how she landed up in Art Science Bangalore. Apart from working on this project, her interests are mythology, catching up with new trends of fashion, experimenting with looks and clothes, food and wildlife.<br />
<br />
<br />
'''Biswajit Manimaran'''<br />
<br />
When you say “Bisu”, his obsession with video games, his laptop, and green shirts are a few things that springs to one’s mind. He’s the “go-to” person for explaining the huge scientific terms that most commoners shy away from, and for explaining most technological terms. He’s quite naturally the resident geek of Art Science Bangalore.<br />
<br />
<br />
'''Damyanti Choudhary'''<br />
<br />
After the joyful moment of passing her science exam for the first time, Damyanti hasn’t looked back at science as a subject since her 10th grade. Through her years since then, her interests developed in interior design, which also led her to join art school. But now, she contemplates the thoughts of pursuing product design, which also became one of her major interests. She’s loud and very short tempered, but has got the perfect skill for organization and research.<br />
<br />
<br />
'''Durga Gawde'''<br />
<br />
Born and raised in an art environment, Durga always knew what her calling was. Science always intrigued her. Although, she hated the way science was taught in the school level her love for science never left her. Igem helped her see new methods of learning, understanding and application. Apart from working on this project, her interests are sports (mainly speed skating), experimenting with photography, listening to powerful music, conversing with different types of people and working out. <br />
<br />
<br />
'''Madhul Singh'''<br />
<br />
Madhul spends her time playing computer games and stumbling on StumbleUpon. An avid reader, she has a special fascination for mythology and wildlife. She hopes to pursue both at some point. She also has a fascination for adventure, and climbs a rock-face like a goat, though coming down is a whole different ballgame (she has Vertigo!). She's the first person you go to when you're troubled and the last person you'd want trouble with. Beware!<br />
<br />
<br />
'''Manasa Thimmiya'''<br />
<br />
Manasa loves music, and travel. Her love for new things and her passion for communication design are what pushed her to do iGem. She is notorious for her brilliant graphics, her interest in social service, and her laziness. <br />
<br />
<br />
'''Prakrithy Pradeep'''<br />
<br />
Prakrithy loves to read and write (everything and anything, except self-help books - they are just plain "Blah") and makes biryani (a preparation of a one-pot meal with rice and meat, laced with exotic spices) to die for. She spends her free time indulging in photography. She dislikes cockroaches and people eating her chocolate, and indulges in using her (always-carry-with-you) Post-Its for anything and everything.<br />
<br />
<br />
'''Pranav Raman'''<br />
<br />
From a very young age Pranav was always interested in science and its applications. Being an art student he has channelized his attempts at finding a harmonious combination of both science and design. It has always fascinated him how if put together, they have done wonders. Looking at science through an artist’s point of view has opened up a whole new world to him. He also has a keen interest music, singing, playing the tabla and flute.<br />
<br />
<br />
'''Sohil Bhatia'''<br />
<br />
Famous as “safety pin boy” and for his wild and crazy ideas-when it comes to his sense of dressing and lifestyle, Sohil brings the crazy side out in Art Science Bangalore. His work ethic and dedication to any project is influenced by his idol- Lady Gaga. His interests include drama, photography and product design.<br />
<br />
<br />
'''Sidharta Guha Thakurta''' <br />
<br />
The “free spirited” one of the team, Sidartha is a born artist who is into expressionist sculpting and painting. He’s enigmatic and has a wild sense of humour and culinary preferences.<br />
<br />
<br />
<br />
<br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2011-10-05T08:08:21Z<p>Achala: </p>
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Art Science Bangalore is a team of 12, Core Skills (2nd year) Art & Design students from Srishti School of Art, Design and Technology, Bangalore. Yashas Shetty, the team facilitator, and Aaron Joseph the team advisor, led the team through the project and research along with the help of Zack Denfeld & Cathrine Kramer . Special thanks to the scientists at National Centre of Biological Sciences (NCBS) – Mukund Tathattai, Ashwin Seshasayee and Navneet Rai for helping us out with the experiments and lab work.<br />
<br />
The profile of each team member is mentioned below:<br />
<br />
<br />
[[File:teamcollage.jpg|500px]]<br />
<br />
<br />
'''Achala Athreya''' <br />
<br />
After joining an art school, Achala never imagined herself ever taking part in a science based competition. The recurring field in the second year of her college life inculcated future design inklings. Her interests however, lean towards textiles, photography and theatre. She enjoys tennis and lately is a maniac about yoga.<br />
<br />
<br />
'''Akshaya Maria Thomas'''<br />
<br />
Easily recognisable because of her wild curly hair, Akshaya is a keen learner. A photographer at heart, she likes experimenting with new methods and techniques. Stationary makes her happy. She enjoys baking and playing chess. She detests clowns and things that crawl.<br />
<br />
<br />
'''Anvita Goenka'''<br />
<br />
Anvita never had interest in biology or any kind of science from her school days and decided to try something different and that is how she landed up in Art Science Bangalore. Apart from working on this project, her interests are mythology, catching up with new trends of fashion, experimenting with looks and clothes, food and wildlife.<br />
<br />
<br />
'''Biswajit Manimaran'''<br />
<br />
When you say “Bisu”, his obsession with video games, his laptop, and green shirts are a few things that springs to one’s mind. He’s the “go-to” person for explaining the huge scientific terms that most commoners shy away from, and for explaining most technological terms. He’s quite naturally the resident geek of Art Science Bangalore.<br />
<br />
<br />
'''Damyanti Choudhary'''<br />
<br />
After the joyful moment of passing her science exam for the first time, Damyanti hasn’t looked back at science as a subject since her 10th grade. Through her years since then, her interests developed in interior design, which also led her to join art school. But now, she contemplates the thoughts of pursuing product design, which also became one of her major interests. She’s loud and very short tempered, but has got the perfect skill for organization and research.<br />
<br />
<br />
'''Durga Gawde'''<br />
<br />
Born and raised in an art environment, Durga always knew what her calling was. Science always intrigued her. Although, she hated the way science was taught in the school level her love for science never left her. Igem helped her see new methods of learning, understanding and application. Apart from working on this project, her interests are sports (mainly speed skating), experimenting with photography, listening to powerful music, conversing with different types of people and working out. <br />
<br />
<br />
'''Madhul Singh'''<br />
<br />
Madhul spends her time playing computer games and stumbling on StumbleUpon. An avid reader, she has a special fascination for mythology and wildlife. She hopes to pursue both at some point. She also has a fascination for adventure, and climbs a rock-face like a goat, though coming down is a whole different ballgame (she has Vertigo!). She's the first person you go to when you're troubled and the last person you'd want trouble with. Beware!<br />
<br />
<br />
'''Manasa Thimmiya'''<br />
<br />
Manasa loves music, and travel. Her love for new things and her passion for communication design are what pushed her to do iGem. She is notorious for her brilliant graphics, her interest in social service, and her laziness. <br />
<br />
<br />
'''Prakrithy Pradeep'''<br />
<br />
Prakrithy loves to read and write (everything and anything, except self-help books - they are just plain "Blah") and makes biryani (a preparation of a one-pot meal with rice and meat, laced with exotic spices) to die for. She spends her free time indulging in photography. She dislikes cockroaches and people eating her chocolate, and indulges in using her (always-carry-with-you) Post-Its for anything and everything.<br />
<br />
<br />
'''Pranav Raman'''<br />
<br />
From a very young age Pranav was always interested in science and its applications. Being an art student he has channelized his attempts at finding a harmonious combination of both science and design. It has always fascinated him how if put together, they have done wonders. Looking at science through an artist’s point of view has opened up a whole new world to him. He also has a keen interest music, singing, playing the tabla and flute.<br />
<br />
<br />
'''Sohil Bhatia'''<br />
<br />
Famous as “safety pin boy” and for his wild and crazy ideas-when it comes to his sense of dressing and lifestyle, Sohil brings the crazy side out in Art Science Bangalore. His work ethic and dedication to any project is influenced by his idol- Lady Gaga. His interests include drama, photography and product design.<br />
<br />
<br />
'''Sidharta Guha Thakurta''' <br />
<br />
The “free spirited” one of the team, Sidartha is a born artist who is into expressionist sculpting and painting. He’s enigmatic and has a wild sense of humour and culinary preferences.<br />
<br />
<br />
<br />
<br />
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</html></div>Achalahttp://2011.igem.org/File:Teamcollage.jpgFile:Teamcollage.jpg2011-10-05T08:07:28Z<p>Achala: </p>
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Art Science Bangalore is a team of 12, Core Skills (2nd year) Art & Design students from Srishti School of Art, Design and Technology, Bangalore. Yashas Shetty, the team facilitator, and Aaron Joseph the team advisor, led the team through the project and research along with the help of Zack Denfeld & Cathrine Kramer . Special thanks to the scientists at National Centre of Biological Sciences (NCBS) – Mukund Tathattai, Ashwin Seshasayee and Navneet Rai for helping us out with the experiments and lab work.<br />
<br />
The profile of each team member is mentioned below:<br />
<br />
<br />
[[File:teamcollage.jpg]]<br />
<br />
<br />
'''Achala Athreya''' <br />
<br />
After joining an art school, Achala never imagined herself ever taking part in a science based competition. The recurring field in the second year of her college life inculcated future design inklings. Her interests however, lean towards textiles, photography and theatre. She enjoys tennis and lately is a maniac about yoga.<br />
<br />
<br />
'''Akshaya Maria Thomas'''<br />
<br />
Easily recognisable because of her wild curly hair, Akshaya is a keen learner. A photographer at heart, she likes experimenting with new methods and techniques. Stationary makes her happy. She enjoys baking and playing chess. She detests clowns and things that crawl.<br />
<br />
<br />
'''Anvita Goenka'''<br />
<br />
Anvita never had interest in biology or any kind of science from her school days and decided to try something different and that is how she landed up in Art Science Bangalore. Apart from working on this project, her interests are mythology, catching up with new trends of fashion, experimenting with looks and clothes, food and wildlife.<br />
<br />
<br />
'''Biswajit Manimaran'''<br />
<br />
When you say “Bisu”, his obsession with video games, his laptop, and green shirts are a few things that springs to one’s mind. He’s the “go-to” person for explaining the huge scientific terms that most commoners shy away from, and for explaining most technological terms. He’s quite naturally the resident geek of Art Science Bangalore.<br />
<br />
<br />
'''Damyanti Choudhary'''<br />
<br />
After the joyful moment of passing her science exam for the first time, Damyanti hasn’t looked back at science as a subject since her 10th grade. Through her years since then, her interests developed in interior design, which also led her to join art school. But now, she contemplates the thoughts of pursuing product design, which also became one of her major interests. She’s loud and very short tempered, but has got the perfect skill for organization and research.<br />
<br />
<br />
'''Durga Gawde'''<br />
<br />
Born and raised in an art environment, Durga always knew what her calling was. Science always intrigued her. Although, she hated the way science was taught in the school level her love for science never left her. Igem helped her see new methods of learning, understanding and application. Apart from working on this project, her interests are sports (mainly speed skating), experimenting with photography, listening to powerful music, conversing with different types of people and working out. <br />
<br />
<br />
'''Madhul Singh'''<br />
<br />
Madhul spends her time playing computer games and stumbling on StumbleUpon. An avid reader, she has a special fascination for mythology and wildlife. She hopes to pursue both at some point. She also has a fascination for adventure, and climbs a rock-face like a goat, though coming down is a whole different ballgame (she has Vertigo!). She's the first person you go to when you're troubled and the last person you'd want trouble with. Beware!<br />
<br />
<br />
'''Manasa Thimmiya'''<br />
<br />
Manasa loves music, and travel. Her love for new things and her passion for communication design are what pushed her to do iGem. She is notorious for her brilliant graphics, her interest in social service, and her laziness. <br />
<br />
<br />
'''Prakrithy Pradeep'''<br />
<br />
Prakrithy loves to read and write (everything and anything, except self-help books - they are just plain "Blah") and makes biryani (a preparation of a one-pot meal with rice and meat, laced with exotic spices) to die for. She spends her free time indulging in photography. She dislikes cockroaches and people eating her chocolate, and indulges in using her (always-carry-with-you) Post-Its for anything and everything.<br />
<br />
<br />
'''Pranav Raman'''<br />
<br />
From a very young age Pranav was always interested in science and its applications. Being an art student he has channelized his attempts at finding a harmonious combination of both science and design. It has always fascinated him how if put together, they have done wonders. Looking at science through an artist’s point of view has opened up a whole new world to him. He also has a keen interest music, singing, playing the tabla and flute.<br />
<br />
<br />
'''Sohil Bhatia'''<br />
<br />
Famous as “safety pin boy” and for his wild and crazy ideas-when it comes to his sense of dressing and lifestyle, Sohil brings the crazy side out in Art Science Bangalore. His work ethic and dedication to any project is influenced by his idol- Lady Gaga. His interests include drama, photography and product design.<br />
<br />
<br />
'''Sidharta Guha Thakurta''' <br />
<br />
The “free spirited” one of the team, Sidartha is a born artist who is into expressionist sculpting and painting. He’s enigmatic and has a wild sense of humour and culinary preferences.<br />
<br />
<br />
<br />
<br />
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</html></div>Achalahttp://2011.igem.org/Team:ArtScienceBangalore/TeamTeam:ArtScienceBangalore/Team2011-10-05T08:01:15Z<p>Achala: </p>
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<br />
Art Science Bangalore is a team of 12, Core Skills (2nd year) Art & Design students from Srishti School of Art, Design and Technology, Bangalore. Yashas Shetty, the team facilitator, and Aaron Joseph the team advisor, led the team through the project and research along with the help of Zack Denfeld & Cathrine Kramer . Special thanks to the scientists at National Centre of Biological Sciences (NCBS) – Mukund Tathattai, Ashwin Seshasayee and Navneet Rai for helping us out with the experiments and lab work.<br />
<br />
The profile of each team member is mentioned below:<br />
<br />
<br />
[[File:achala.jpg|150px]][[File:akshaya.jpg|150px]][[File:anvita.jpg|150px]][[File:[[File:bisu.jpg|150px]][[File:damyanti.jpg|150px]][[File:durga.jpg|150px][[File:madhul.jpg][[File:manasa.jpg][[File:prakrithy.jpg][[File:pranav.jpg][[File:sidartha.jpg][[File:sohil.jpg]]<br />
<br />
<br />
'''Achala Athreya''' <br />
<br />
After joining an art school, Achala never imagined herself ever taking part in a science based competition. The recurring field in the second year of her college life inculcated future design inklings. Her interests however, lean towards textiles, photography and theatre. She enjoys tennis and lately is a maniac about yoga.<br />
<br />
<br />
'''Akshaya Maria Thomas'''<br />
<br />
Easily recognisable because of her wild curly hair, Akshaya is a keen learner. A photographer at heart, she likes experimenting with new methods and techniques. Stationary makes her happy. She enjoys baking and playing chess. She detests clowns and things that crawl.<br />
<br />
<br />
'''Anvita Goenka'''<br />
<br />
Anvita never had interest in biology or any kind of science from her school days and decided to try something different and that is how she landed up in Art Science Bangalore. Apart from working on this project, her interests are mythology, catching up with new trends of fashion, experimenting with looks and clothes, food and wildlife.<br />
<br />
<br />
'''Biswajit Manimaran'''<br />
<br />
When you say “Bisu”, his obsession with video games, his laptop, and green shirts are a few things that springs to one’s mind. He’s the “go-to” person for explaining the huge scientific terms that most commoners shy away from, and for explaining most technological terms. He’s quite naturally the resident geek of Art Science Bangalore.<br />
<br />
<br />
'''Damyanti Choudhary'''<br />
<br />
After the joyful moment of passing her science exam for the first time, Damyanti hasn’t looked back at science as a subject since her 10th grade. Through her years since then, her interests developed in interior design, which also led her to join art school. But now, she contemplates the thoughts of pursuing product design, which also became one of her major interests. She’s loud and very short tempered, but has got the perfect skill for organization and research.<br />
<br />
<br />
'''Durga Gawde'''<br />
<br />
Born and raised in an art environment, Durga always knew what her calling was. Science always intrigued her. Although, she hated the way science was taught in the school level her love for science never left her. Igem helped her see new methods of learning, understanding and application. Apart from working on this project, her interests are sports (mainly speed skating), experimenting with photography, listening to powerful music, conversing with different types of people and working out. <br />
<br />
<br />
'''Madhul Singh'''<br />
<br />
Madhul spends her time playing computer games and stumbling on StumbleUpon. An avid reader, she has a special fascination for mythology and wildlife. She hopes to pursue both at some point. She also has a fascination for adventure, and climbs a rock-face like a goat, though coming down is a whole different ballgame (she has Vertigo!). She's the first person you go to when you're troubled and the last person you'd want trouble with. Beware!<br />
<br />
<br />
'''Manasa Thimmiya'''<br />
<br />
Manasa loves music, and travel. Her love for new things and her passion for communication design are what pushed her to do iGem. She is notorious for her brilliant graphics, her interest in social service, and her laziness. <br />
<br />
<br />
'''Prakrithy Pradeep'''<br />
<br />
Prakrithy loves to read and write (everything and anything, except self-help books - they are just plain "Blah") and makes biryani (a preparation of a one-pot meal with rice and meat, laced with exotic spices) to die for. She spends her free time indulging in photography. She dislikes cockroaches and people eating her chocolate, and indulges in using her (always-carry-with-you) Post-Its for anything and everything.<br />
<br />
<br />
'''Pranav Raman'''<br />
<br />
From a very young age Pranav was always interested in science and its applications. Being an art student he has channelized his attempts at finding a harmonious combination of both science and design. It has always fascinated him how if put together, they have done wonders. Looking at science through an artist’s point of view has opened up a whole new world to him. He also has a keen interest music, singing, playing the tabla and flute.<br />
<br />
<br />
'''Sohil Bhatia'''<br />
<br />
Famous as “safety pin boy” and for his wild and crazy ideas-when it comes to his sense of dressing and lifestyle, Sohil brings the crazy side out in Art Science Bangalore. His work ethic and dedication to any project is influenced by his idol- Lady Gaga. His interests include drama, photography and product design.<br />
<br />
<br />
'''Sidharta Guha Thakurta''' <br />
<br />
The “free spirited” one of the team, Sidartha is a born artist who is into expressionist sculpting and painting. He’s enigmatic and has a wild sense of humour and culinary preferences.<br />
<br />
<br />
<br />
<br />
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</div><br />
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</html></div>Achalahttp://2011.igem.org/File:Damyanti.jpgFile:Damyanti.jpg2011-10-05T07:59:43Z<p>Achala: </p>
<hr />
<div></div>Achalahttp://2011.igem.org/File:Bisu.jpgFile:Bisu.jpg2011-10-05T07:59:17Z<p>Achala: </p>
<hr />
<div></div>Achala